Electrophysiological responses of the olfactory receptors of the tick Amblyomma cajennense (Acari: Ixodidae) to host-related and tick pheromone-related synthetic compounds

Soares, Sara Fernandes; Borges, Lígia Miranda Ferreira

doi:10.1016/j.actatropica.2012.08.007

Cited by 37 publications

(31 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A. cajennense [90], D. andersoni , D. variabilis [85], and R. sanguineus [90] also exhibit attractive behavior when exposed to 2,6-DCP in olfactometer bioassays. Electrophysiological studies determined that sensilla in the anterior pit of the Haller's organ of A. cajennense [91], A. variegatum , D. variabilis , and R. appendiculatus [92] immature and mature males are responsible for detecting 2,6-DCP.…”

Section: Chemistry Of Reproductive (Sex) Pheromonesmentioning

confidence: 99%

Acarine attractants: Chemoreception, bioassay, chemistry and control

Carr

Roe

2016

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

The Acari are of significant economic importance in crop production and human and animal health. Acaricides are essential for the control of these pests, but at the same time, the number of available pesticides is limited, especially for applications in animal production. The Acari consist of two major groups, the mites that demonstrate a wide variety of life strategies, i.e., herbivory, predation and ectoparasitism, and ticks which have evolved obligatory hematophagy. The major sites of chemoreception in the acarines are the chelicerae, palps and tarsi on the forelegs. A unifying name, the “foretarsal sensory organ” (FSO), is proposed for the first time in this review for the sensory site on the forelegs of all acarines. The FSO has multiple sensory functions including olfaction, gustation, and heat detection. Preliminary transcriptomic data in ticks suggest that chemoreception in the FSO is achieved by a different mechanism from insects. There are a variety of laboratory and field bioassay methods that have been developed for the identification and characterization of attractants but minimal techniques for electrophysiology studies. Over the past three to four decades, significant progress has been made in the chemistry and analysis of function for acarine attractants in mites and ticks. In mites, attractants include aggregation, immature female, female sex and alarm pheromones; in ticks, the attraction–aggregation–attachment, assembly and sex pheromones; in mites and ticks host kairomones and plant allomones; and in mites, fungal allomones. There are still large gaps in our knowledge of chemical communication in the acarines compared to insects, especially relative to acarine pheromones, and more so for mites than ticks. However, the use of lure-and-kill and lure-enhanced biocontrol strategies has been investigated for tick and mite control, respectively, with significant environmental advantages which warrant further study.

show abstract

Section: Chemistry Of Reproductive (Sex) Pheromonesmentioning

confidence: 99%

Acarine attractants: Chemoreception, bioassay, chemistry and control

Carr

Roe

2016

Pesticide Biochemistry and Physiology

View full text Add to dashboard Cite

show abstract

“…ricinus (Leonovich 2004), A. variegatum (Steullet and Guerin 1994b) and A. cajennese (Soares and Borges 2012). In most of these studies, ORNs from more than one sensillum responded to the pheromone (s) (Leonovich 1990).…”

Section: Discussionmentioning

confidence: 99%

Neurophysiological and Behavioral Responses ofIxodes scapularisto host odors

Josek¹,

Sperrazza

Alleyne

et al. 2020

Preprint

View full text Add to dashboard Cite

The black-legged tick, Ixodes scapularis (Ixodida, Ixodidae), is one of the major disease vectors in the United States and due to multiple human impact factors, such as decreasing forest size for land development and climate change, it has expanded its range and established across the United States. Throughout the life cycle, ticks locate hosts for their blood-meal and although the ecologies of this tick and their hosts have been studied in depth, the sensory physiology behind host location largely remains unexplored. Here we report establishing a robust paradigm to isolate and identify odors from the natural milieu for I. scapularis. We performed single sensillum recordings (SSR) from the olfactory sensilla on the tick tarsi, and used the SSR system as biological detector to isolate natural compounds that elicited biological activity. The SSR setup was further tested in tandem with gas chromatography (GC) wherein the ticks’ olfactory sensillum activity served as a biological detector. The GC-SSR recordings from the wall pore sensilla in the Haller’s organ, and further identification of the biologically active deer glad constituents by GC-mass spectrometry (GC-MS) revealed methyl substituted phenols as strong chemostimuli, as compared to ethyl or propyl substitutions. Strongest electrophysiological activity was elicited by meta-cresol followed by para-cresol. Ethyl- and propylphenols with any of the three, ortho, meta or para substitutions, did not induce any neurophysiological activity. Finally, a behavioral analysis in a dual-choice olfactometer of all these phenols at three different doses revealed no significant behavioral response, except for p-cresol at −3 dilution Overall, this study contributes to our understanding of I. scapularis tick’s neurophysiology and provides a robust platform to isolate and identify natural attractants and repellents.

show abstract

“…Compared to insects, chemical communication in other arthropods, particularly Chelicerata, is poorly understood. Most of the studies are focused on morphology 11 and electrophysiology [12][13][14] while several papers report on the identification of putative semiochemicals 8,9,[15][16][17][18] .…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: a comprehensive examination of the potential carriers for semiochemicals

Iovinella

McAfee

Mastrobuoni

et al. 2018

Preprint

View full text Add to dashboard Cite

The mite Varroa destructor is the major parasite of the honey bee and is responsible for great economical losses. The biochemical tools used by Varroa to detect semiochemicals produced by the host are still largely unknown. We have performed proteomic analysis on chemosensory organs of this species in order to identify putative soluble carriers for pheromones and other olfactory cues emitted by the host. In particular, we have analysed forelegs, mouthparts (palps, chelicera and hypostome) and the second pair of legs (as control tissue) in reproductive and phoretic stages of the Varroa life cycle. We identified 958 Varroa proteins, most of them common to organs and stages. Sequence analysis shows that four proteins can be assigned to the odorant-binding protein (OBP)-like class, which bear some similarity to insect OBPs, but so far are only reported in some Chelicerata. In addition, we have detected the presence of two proteins belonging to the Niemann-Pick family, type C2

show abstract

Electrophysiological responses of the olfactory receptors of the tick Amblyomma cajennense (Acari: Ixodidae) to host-related and tick pheromone-related synthetic compounds

Cited by 37 publications

References 43 publications

Acarine attractants: Chemoreception, bioassay, chemistry and control

Acarine attractants: Chemoreception, bioassay, chemistry and control

Neurophysiological and Behavioral Responses ofIxodes scapularisto host odors

Proteomic analysis of chemosensory organs in the honey bee parasite Varroa destructor: a comprehensive examination of the potential carriers for semiochemicals

Contact Info

Product

Resources

About