Electrophysiological Monitoring of Hippocampal Slice Cultures Using MEA on Porous Membrane

Hakkoum, David; Michelet, Dominique; Stoppini, Luc

doi:10.1007/0-387-25858-2_5

Cited by 4 publications

(3 citation statements)

References 53 publications

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“…Technological advancements continue to increase the number of applications for MEAs and improve their functionality. Specialized MEA designs include perforated arrays for long-term organotypic brain slice culturing , and arrays with custom electrode geometries to match neuroanatomical structures . MEAs have also been designed to interrogate neural function at the single cell level with electrodes surrounded by microcages for spatially confining neurons or at the subcellular level with electrodes as small as 2 μm or even fabricated from silicon nanowires …”

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confidence: 99%

“…The surface of acutely sectioned tissue slices is comprised of injured or dead cells, and the surface of slice cultures may consist of a reactive glia layer; both reduce signal propagation from the active cells to the electrodes . To improve signal quality, two groups have designed and tested MEAs with three- dimensional (3-D) electrodes to penetrate into the tissue. ,,, The Ayanda Biosystems arrays , and the other − employ pyramidal platinum electrodes approximately 50 μm tall for recording and stimulation.…”

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Vertically Aligned Carbon Nanofiber Arrays Record Electrophysiological Signals from Hippocampal Slices

et al. 2007

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Vertically aligned carbon nanofiber (VACNF) electrode arrays were tested for their potential application in recording neuro-electrophysiological activity. We report, for the first time, stimulation and extracellular recording of spontaneous and evoked neuroelectrical activity in organotypic hippocampal slice cultures with ultramicroelectrode VACNF arrays. Because the electrodes are carbon-based, these arrays have potential advantages over metal electrodes and could enable a variety of future applications as precise, informative, and biocompatible neural interfaces.

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Vertically Aligned Carbon Nanofiber Arrays Record Electrophysiological Signals from Hippocampal Slices

et al. 2007

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“…Each electrode can be assigned as stimulating or recording. Recording session was performed at day five post‐ischemia (Hakkoum et al. 2006, 2007).…”

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confidence: 99%

Clusterin increases post‐ischemic damages in organotypic hippocampal slice cultures

Hakkoum

Imhof

Vallet

et al. 2008

Journal of Neurochemistry

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Clusterin or apolipoprotein J is a heterodimeric glycoprotein which is known to be increased during tissue involution in response to hormonal changes or injury and under circumstances leading to apoptosis. Previous studies in wild-type (WT) and clusterin-null (Clu-/-) mice indicated a protective role of clusterin over-expression in astrocytes lasting up to 90 days post-ischemia. However, in in vitro and in vivo models of neonatal hypoxia-ischemia, clusterin exacerbates necrotic cell death. We developed recombinant forms of clusterin and examined their effect on propidium iodide uptake, neuronal and synaptic markers as well as electrophysiological recordings in hippocampal slice cultures from Clu-/- and WT mice subjected to oxygen-glucose deprivation (OGD). WT mice displayed a marked up-regulation of clusterin associated with electrophysiological deficits and dramatic increase of propidium iodide uptake 5 days post-OGD. Immunocytochemical and western blot analyses revealed a substantial decrease of neuronal nuclei and synaptophysin immunoreactivity that predominated in WT mice. These findings contrasted with the relative post-OGD resistance of Clu-/- mice. The addition of biologically active recombinant forms of human clusterin for 24 h post-OGD led to the abolishment of the ischemic tolerance in Clu-/- slices. This deleterious effect of clusterin was reverted by the concomitant administration of the NMDA receptor antagonist, d-2-amino-5-phosphonopentanoate. The present data indicate that in an in vitro model of ischemia characterized by the predominance of NMDA-mediated cell death, clusterin exerts a negative effect on the structural integrity and functionality of hippocampal neurons

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Organotypic microtissues on an air‐liquid interface

Sundström

Charvet

Stoppini

2017

Technology Platforms for 3D Cell Culture

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Electrophysiological Monitoring of Hippocampal Slice Cultures Using MEA on Porous Membrane

Cited by 4 publications

References 53 publications

Vertically Aligned Carbon Nanofiber Arrays Record Electrophysiological Signals from Hippocampal Slices

Vertically Aligned Carbon Nanofiber Arrays Record Electrophysiological Signals from Hippocampal Slices

Clusterin increases post‐ischemic damages in organotypic hippocampal slice cultures

Organotypic microtissues on an air‐liquid interface

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