Electrophysiological characterization of human Na+/taurocholate cotransporting polypeptide (hNTCP) heterologously expressed in Xenopus laevis oocytes

Masuda, Masayuki; Ichikawa, Yasumitsu; Shimono, Kazumi; Shimizu, Masaru; Tanaka, Yoshio; Nara, Toshifumi; Miyauchi, Seiji

doi:10.1016/j.abb.2014.08.010

Cited by 5 publications

(3 citation statements)

References 49 publications

(108 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Hepatocytes were isolated from the livers of 6-week-old male SD rats that were given free access to food and water. The isolation procedure was basically the same as previously reported (Miyauchi et al, 1993;Masuda et al, 2014). Cell viability, determined by Trypan blue exclusion, ranged from 95 to 98%.…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

The Phenomenon of Albumin-Mediated Hepatic Uptake of Organic Anion Transport Polypeptide Substrates: Prediction of the In Vivo Uptake Clearance from the In Vitro Uptake by Isolated Hepatocytes Using a Facilitated-Dissociation Model

Miyauchi¹,

Masuda²,

Kim³

et al. 2018

Drug Metab Dispos

Self Cite

View full text Add to dashboard Cite

The effects of bovine serum albumin and human serum albumin on the unbound hepatic uptake clearance () of the organic anion-transporting polypeptide substrates 1-anilino-8-naphthalene sulfonate (ANS) and pitavastatin (PTV) were determined using primary cultured rat hepatocytes and isolated human hepatocytes, respectively. The value of hepatocytes was estimated by dividing the initial uptake rate of these anions by their unbound concentrations. The values for ANS and PTV were enhanced in the presence of albumin, thereby demonstrating the phenomenon of "albumin-mediated" hepatic uptake. We previously constructed a "facilitated-dissociation" model, in which the interaction of the ligand-albumin complex with the cell surface enhanced the dissociation of that complex to provide unbound ligand for uptake to the hepatocytes [ 16:165-181 (1988)]. That model was able to describe accurately the relationship between the enhancement of the values and the albumin concentration. By considering the enhancement of hepatic uptake clearance by albumin using this facilitated-dissociation model, we could predict accurately the in vivo from that obtained in isolated hepatocytes. In the light of these findings, we suggest that the facilitated-dissociation model is applicable to describing the phenomenon of albumin-mediated hepatic uptake via organic anion transporters and to evaluating hepatic uptake clearance in vivo.

show abstract

Section: Methodsmentioning

confidence: 99%

“…Uptake of ANS in primary cultured hepatocytes was measured at 37°C in uptake buffer (pH 7.5) as described previously (Masuda et al, 2014). The composition of the uptake buffer was 140 mM NaCl, 3 mM KCl, 1 mM CaCl 2 , 1 mM MgCl 2 , 5 mM glucose, and 10 mM Hepes/Tris adjusted to pH 7.5.…”

Section: Methodsmentioning

confidence: 99%

The Phenomenon of Albumin-Mediated Hepatic Uptake of Organic Anion Transport Polypeptide Substrates: Prediction of the In Vivo Uptake Clearance from the In Vitro Uptake by Isolated Hepatocytes Using a Facilitated-Dissociation Model

Miyauchi¹,

Masuda²,

Kim³

et al. 2018

Drug Metab Dispos

Self Cite

View full text Add to dashboard Cite

show abstract

“…Transport of bile acids is driven by the inwardly directed Na + gradient, maintained by basolateral Na + -and K + -ATPase, and the negative intracellular potential. Bile acids and transportable substrates, such as rosuvastatin and fluvastatin, induce inward currents (Masuda et al 2014, Weinman 1997. Unlike bile acid transport, which strictly depends on the Na + gradient crossing the cell membrane, the Na + gradient or the inward Na + current per se is dispensable for NTCP-mediated HBV/HDV viral entry (Yan et al 2014).…”

Section: Ntcp May Undergo Endocytosis To Fulfill Its Role As a Viral mentioning

confidence: 98%

The Hepatitis B Virus Receptor

2015

Annu. Rev. Cell Dev. Biol.

View full text Add to dashboard Cite

Hepatitis B virus (HBV) infection affects 240 million people worldwide. A liver-specific bile acid transporter named the sodium taurocholate cotransporting polypeptide (NTCP) has been identified as the cellular receptor for HBV and its satellite, the hepatitis D virus (HDV). NTCP likely acts as a major determinant for the liver tropism and species specificity of HBV and HDV at the entry level. NTCP-mediated HBV entry interferes with bile acid transport in cell cultures and has been linked with alterations in bile acid and cholesterol metabolism in vivo. The human liver carcinoma cell line HepG2, complemented with NTCP, now provides a valuable platform for studying the basic biology of the viruses and developing treatments for HBV infection. This review summarizes critical findings regarding NTCP's role as a viral receptor for HBV and HDV and discusses important questions that remain unanswered.

show abstract

Transport of 2,4-dichloro phenoxyacetic acid by human Na+-coupled monocarboxylate transporter 1 (hSMCT1, SLC5A8)

Sugio

Inoda

Masuda

et al. 2019

Drug Metabolism and Pharmacokinetics

View full text Add to dashboard Cite

Electrophysiological characterization of human Na+/taurocholate cotransporting polypeptide (hNTCP) heterologously expressed in Xenopus laevis oocytes

Cited by 5 publications

References 49 publications

The Phenomenon of Albumin-Mediated Hepatic Uptake of Organic Anion Transport Polypeptide Substrates: Prediction of the In Vivo Uptake Clearance from the In Vitro Uptake by Isolated Hepatocytes Using a Facilitated-Dissociation Model

The Phenomenon of Albumin-Mediated Hepatic Uptake of Organic Anion Transport Polypeptide Substrates: Prediction of the In Vivo Uptake Clearance from the In Vitro Uptake by Isolated Hepatocytes Using a Facilitated-Dissociation Model

The Hepatitis B Virus Receptor

Transport of 2,4-dichloro phenoxyacetic acid by human Na+-coupled monocarboxylate transporter 1 (hSMCT1, SLC5A8)

Contact Info

Product

Resources

About