Electrophysiological and behavioural responses of the Eucalyptus weevil, Gonipterus platensis, to host plant volatiles

Mateus, Eduardo P.; Silva, Marco D.R. Gomes da; Mendes, Davide; Rocha, Sı́lvia M.; Mendel, Zvi; Schütz, Stefan; Paiva, Maria Rosa

doi:10.1007/s10340-018-1055-0

Cited by 15 publications

(29 citation statements)

References 46 publications

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“…Coelutions occurred for cis ‐verbenol + trans ‐pinocarveol + alloaromadendrene, and myrtenol + 7‐hydroxy‐1,8‐cineole. Confirmation of the electrophysiological activity of the coeluted compounds allo‐ aromadendrene, trans ‐pinocarveol and myrtenol was reported in a previous study (Branco et al, ). Compounds, 2‐α ‐hydroxy‐1,8‐cineole and 9‐hydroxy‐1,8‐cineole, showed a resolution lower than 1, due to the high concentration of the 2 hydroxyl isomer.…”

Section: Resultssupporting

confidence: 80%

“…Figure shows an example of a chromatogram obtained from G. platensis virgin males on the GC/TOFMS system (system 4, Section 2.3). The majority of the volatile compounds identified in the insects (male or female) headspace were also found in E. globulus (Branco et al, ). Eleven compounds were only detected in the headspace of samples containing G. platensis (Table ).…”

Section: Resultsmentioning

confidence: 99%

“…A culture of G. platensis was established in a laboratory in the NOVA School of Science and Technology (FCT NOVA), NOVA University Lisbon, Caparica, Portugal. The rearing methodology adopted, described in Branco et al (), allowed for the obtention of insects of known age, sex and mating status, to be used in the assays. All insects used in this study were aged between 1 and 4 months.…”

Section: Methodsmentioning

confidence: 99%

“…EAD‐active peaks were identified by calculating the average of the electrophysiological signals and plotting them against the corresponding chromatographic data. A reduction of the random noise present in individual records was achieved after standardization of each EAD, by applying the formula x′ = (x −

\bar{x}

)/δ to all data points of each recording, where x = recorded signal;

\bar{x}

= mean of the recording signal for the entire run; δ = standard deviation, see Branco et al (). The average of the standardized signals was calculated for each sex and each extract tested.…”

Section: Methodsmentioning

confidence: 99%

“…The study presented here is part of a comprehensive project aiming at decoding the chemical ecology of G. platensis , regarding the responses to both pheromones and to host volatiles. The complete characterization of E. globulus emitted volatiles and the weevils electrophysiological and behavioural responses to host volatiles were reported in Branco et al (). The objectives of the current study were to detect and identify volatile chemicals, emitted exclusively by the weevils that may act as potential pheromones and to test them in the laboratory in behavioural and electrophysiological bioassays.…”

Section: Introductionmentioning

confidence: 94%

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Identification of pheromone candidates for the eucalyptus weevil, Gonipterus platensis (Coleoptera, Curculionidae)

Mateus

Silva

Mendes

et al. 2019

J Applied Entomology

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The eucalyptus weevil, Gonipterus platensis (Coleoptera, Curculionidae), is a major pest of eucalyptus plantations worldwide. To date, no pheromones have been identified for this species, despite their valuable potential as tools in monitoring or control strategies. Here we report the detection and identification of pheromones candidates of G. platensis. The weevil's volatile compounds were collected by solid phase micro extraction (SPME) and monolithic material sorption extraction (MMSE). Using Gas Chromatography coupled to Mass Spectrometry (GC/MS) analysis, eleven insect specific compounds were detected and identified: verbenene, cis‐verbenol, trans‐verbenol, verbenone, 2‐oxo‐1,8‐cineole, 9‐hydroxy‐1,8‐cineole, 2‐α‐hydroxy‐1,8‐cineole, 3‐oxo‐1,8‐cineole, 2‐β‐hydroxy‐1,8‐cineole, 3‐α‐hydroxy‐1,8‐cineole and 7‐hydroxy‐1,8‐cineole. Three of these compounds, verbenene, cis‐verbenol and trans‐verbenol, were shown to be male‐specific. Antennal sensitivity towards ten compounds emitted by G. platensis was detected using Gas Chromatography–Mass Spectrometry/Electroantennographic Detection (GC‐MS/EAD). Extracts from virgin males proved to be attractive to virgin females in olfactometer bioassays. Further behavioural bioassays showed that both virgin females and virgin males were attracted to the male‐specific compound cis‐verbenol and that virgin females were attracted to trans‐verbenol. Verbenone was attractive to mated females. Regarding 2‐α‐hydroxy‐1.8‐cineole and 2‐oxo‐1,8‐cineole, which are produced by both sexes, the alcohol was attractive to virgin males and both the alcohol and the ketone were repellant to mated females. This is, to our knowledge, the first identification of pheromones candidates in Gonipterus spp. and also the first evidence of cineole metabolites acting as semiochemicals.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

\bar{x}

)/δ to all data points of each recording, where x = recorded signal;

\bar{x}

= mean of the recording signal for the entire run; δ = standard deviation, see Branco et al (). The average of the standardized signals was calculated for each sex and each extract tested.…”

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 94%

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