Electrophoretic variants characteristic of heterozygotes in haploid laver Porphyra sp.

Abstract

Starch gel electrophoresis was carried out to survey the genetic variation in the haploid lavers of the Porphyra sp. claimed to be Porphyra renera. Eleven enzyme loci were resolved and scored. Eight of the eleven loci were monomorphic, while the three loci, catalase (CAT), glucosephosphate isomerase (GPI) and mannosephosphate isomerase (MPI) showed polymorphism. At the Gpi and Mpi loci, a number of lavers had electrophoretic phenotypes characteristic of heterozygotes. In order to compare with Porphyra yezoensis, the allelic frequencies were calculated on the assumption that the heterozygous phenotypes possessed two alleles. The genetic distance between Porphyra sp. and Porphyra yezoensis was 0.1511, indicating the differentiation of the subspecies level. Electrophoretic phenotypes characteristic of heterozygotes is unexpected, since Porphyra lavers are in a haploid phase. Our findings suggest that the plant is either in the diploid phase or that duplication occurs in one of the chromosome sets. 1.1.1.44.), phosphoglucomutase (PGM 2.7.5.1.),

Genetic polymorphism withinPorphyra yezoensis(Bangiales, Rhodophyta) and related species from Japan and Korea detected by cleaved amplified polymorphic sequence analysis

“…These differences suggest the possibility that the JHS strain has incidentally acquired a diploid heterokaryon genotype caused by chromosome reorganization, including a fission/fusion process ultimately resulting in cross-fertilization in nature. Fujio et al (1988) reported electrophoretic phenotype characteristics of heterozygotes in the haploid phase of Porphyra sp. and suggested that the plant may be either in the diploid phase or that duplication had occurred in one of the chromosome sets.…”

Biogeographic Population Structure of Chimeric Blades of Porphyra in the Northeast Atlantic Reveals Southern Rich Gene Pools, Introgression and Cryptic Plasticity

“…In addition, some species display self-fertilization and asexual reproduction (Miura et al, 1979;Fujio et al, 1985Fujio et al, , 1987Gil-Kodaka et al, 1988). Another challenge is the presence of multiple alleles in supposedly haploid gametophytic blades, which can be influenced by divergent partial or complete chromosome copies (Fujio et al, 1988;Varela-Álvarez et al, 2018bVarela-Álvarez et al, , 2021, in chimeric blades (Lindstrom, 1993;Varela-Álvarez et al, 2021) that retain multiple alleles from the conchospores that originated them.…”