Electrophoretic techniques: the old and the new

Counotte, G. H. M.

doi:10.1111/j.1939-165x.2010.00279.x

Cited by 4 publications

(3 citation statements)

References 22 publications

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“…Urinary capillary electrophoresis is a technique that allows the separation and quantification of proteins in urine by applying an electric field through a silica capillary. It has proven to be a sensitive specific tool for detecting and characterizing different protein fractions in urine, including albumin, globulins, and other low-molecular-weight proteins [2][3][4]. Urinary capillary electrophoresis has been used in studies to assess proteinuria in dogs, providing information on protein profiles and possible alterations associated with renal and systemic diseases [25].…”

Section: Comparison Between Urinary and Serum Electrophoretograms In ...mentioning

confidence: 99%

“…In addition, it allows a faster separation of proteins and a better characterization of protein profiles. On the other hand, agarose gel electrophoresis is widely accessible, cheaper, and offers a high resolution of protein bands, which facilitates the identification of specific proteins [2][3][4]28,29]. Therefore, urinary capillary electrophoresis and agarose gel electrophoresis are helpful techniques for evaluating proteinuria in dogs and cats.…”

Section: Comparison Between Urinary and Serum Electrophoretograms In ...mentioning

confidence: 99%

“…Electrophoresis can be classified as zonal or capillary. Zonal electrophoresis requires a solid support such as a porous gel, usually a polymer (agarose or acrylamide); conversely, capillary electrophoresis (CE) uses silica capillaries to separate the biomolecules [2][3][4].…”

Section: Introductionmentioning

confidence: 99%

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Urinary Electrophoretograms Performed by Capillary Electrophoresis: Comparison between Dogs and Cats

Gil,

Wsol,

Fernández-Barredo

et al. 2023

Gels

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Electrophoresis of urine to evaluate different urinary proteins has been used in recent years in veterinary medicine, as it can be a useful laboratory tool in the early detection of kidney damage. However, urinary capillary electrophoresis (UCE) has not been reported in healthy cats. In healthy dogs, reference intervals have been established and can be compared with pathological samples as these provide an easily interpretable pattern. The electrophoretogram in this study is divided into five fractions (F1–F5) by serum (albumin; alpha1-globulin; alpha2-globulin; beta-globulin; and gamma-globulin). Urine samples from 14 healthy cats were obtained by eco-guided cystocentesis. UCE was run in all samples and compared to 123 dog electrophoretograms from a previously published study. Fraction 2 (alpha1-globulin) was statistically decreased in cats (G1) compared to dogs (G2). Fraction 4 (beta-globulin) was statistically augmented in cats compared to the canine population (G2). Fraction 5 (gamma-globulin) was statistically decreased in cats (G1) compared to dogs (G2). No statistical correlation was found between each cat’s serum and urinary fractions. The results of the present study suggest that UCE patterns in cats are similar to the ones described in dogs. UCE can be a non-invasive new diagnostic tool in cats as pathological patterns can be compared to normal ones.

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Section: Comparison Between Urinary and Serum Electrophoretograms In ...mentioning

confidence: 99%

Section: Comparison Between Urinary and Serum Electrophoretograms In ...mentioning

confidence: 99%

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Urinary Electrophoretograms Performed by Capillary Electrophoresis: Comparison between Dogs and Cats

Gil,

Wsol,

Fernández-Barredo

et al. 2023

Gels

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Reference intervals for electrophoretograms obtained by capillary electrophoresis of dialyzed urine from healthy dogs

Navarro

Gil²,

Martin³

et al. 2021

J VET Diagn Invest

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Electrophoresis of urine to evaluate protein fractions in dogs with proteinuria to differentiate glomerular from tubular damage has increased in recent years; however, capillary electrophoresis (CE) of urine has not been reported in a study of > 40 healthy animals, to our knowledge. We aimed to establish reference intervals (RIs) for the urine protein fractions obtained by CE of urine from healthy dogs. We obtained urine samples from 123 clinically healthy dogs of both sexes between December 2016 and April 2019; urine was frozen until CE was performed. The electrophoretic patterns obtained were divided into 5 protein fractions, and RIs were established in percentages and absolute values using nonparametric methods. RIs were obtained for the fractions (F) as follows: 5.5 to 56.2% for F1, 3.2 to 16.5% for F2, 3.5 to 16.2% for F3, 17.8 to 69.8% for F4, and 5.1 to 23.9% for F5. These RIs obtained by CE might be useful clinically as a basis for comparison with pathologic samples. Age was a statistically significant factor for F2 ( p = 0.01) and F3 ( p = 0.02), and sex was a statistically significant factor for F1 ( p = 0.03).

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Comparison of Serum Protein Electrophoresis Values in Wild and Captive Whooping Cranes (Grus americana)

Hausmann¹,

Cray²,

Hartup³

2015

Journal of Avian Medicine and Surgery

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Protein electrophoresis of serum samples from endangered, wild whooping cranes ( Grus americana ) was performed to help assess the health of the only self-sustaining, migratory population in North America. Serum samples from wild adult cranes (n = 22) were taken at Aransas National Wildlife Refuge, Texas, USA during winter. Wild juvenile cranes (n = 26) were sampled at Wood Buffalo National Park, Northwest Territories, Canada, in midsummer. All captive crane samples were acquired from the International Crane Foundation, Baraboo, WI, USA. Captive adult cranes (n = 30) were sampled during annual examinations, and archived serum samples from captive juvenile cranes (n = 19) were selected to match the estimated age of wild juveniles. Wild juveniles had significantly lower concentrations of all protein fractions than wild adults, except for prealbumin and γ globulins. All protein fraction concentrations for wild juveniles were significantly lower compared with captive juveniles, except for prealbumin and γ globulins, which were higher. Wild adults had significantly greater γ globulin concentrations than captive adults. Captive juveniles had significantly lower prealbumin and albumin concentrations and albumin : globulin ratios than captive adults. The higher γ globulin concentrations in wild versus captive cranes are likely because of increased antigenic exposure and immune stimulation. Protein fraction concentrations vary significantly with age and natural history in this species. Reference intervals for serum protein electrophoresis results from captive adult whooping cranes are provided in this study.

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Electrophoretic techniques: the old and the new

Cited by 4 publications

References 22 publications

Urinary Electrophoretograms Performed by Capillary Electrophoresis: Comparison between Dogs and Cats

Urinary Electrophoretograms Performed by Capillary Electrophoresis: Comparison between Dogs and Cats

Reference intervals for electrophoretograms obtained by capillary electrophoresis of dialyzed urine from healthy dogs

Comparison of Serum Protein Electrophoresis Values in Wild and Captive Whooping Cranes (Grus americana)

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