Electrophoretic techniques in element species analysis. Comparison between flow-through and flat-bed techniques

Hinkamp, S.; Dunemann, Lothar

doi:10.1016/s0003-2670(00)82753-1

Cited by 7 publications

(1 citation statement)

References 6 publications

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“…Flatbed native-PAGE was shown by Dunemann et al [26,27] to be a preferred technique to gel chromatography and flow-through electrophoresis for the separation of metal-protein complexes in soybean flour extracts. Following gel electroelution, flame AAS was used to establish distribution profiles for Cu, Ni, and Zn.…”

Section: Metalloprotein Speciation Studies 41 Atomic Absorption Specmentioning

confidence: 99%

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

McLeod

Tomlinson

et al. 2004

Electrophoresis

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The metabolism of trace elements, in particular their binding to proteins in biological systems is of great importance in biochemical, toxicological, and pharmacological studies. As a result there has been a sustained interest over the last two decades in the speciation of protein-bound metals. Various analytical approaches have been employed, combining efficient separation of metalloproteins by liquid chromatography or electrophoresis with high-sensitivity elemental detection. Slab-gel electrophoresis (GE) is a key platform for high-resolution protein separation, and has been combined with autoradiography and various atomic spectrometric techniques for in-gel determination of protein-bound metals. Recently, the combination of GE with state-of-the-art inductively coupled plasma-mass spectrometry (ICP-MS), particularly when linked to laser ablation (LA) for direct gel interrogation, has opened up new opportunities for rapid characterization of metalloproteins. The use of GE and atomic spectrometry for the speciation of protein-bound trace elements is reviewed in this paper. Technical requirements for gel electrophoresis/atomic spectrometric measurement are considered in terms of method compatibilities, detection capability and potential usefulness. The literature is also surveyed to illustrate current status and future trends.

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Section: Metalloprotein Speciation Studies 41 Atomic Absorption Specmentioning

confidence: 99%

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

McLeod

Tomlinson

et al. 2004

Electrophoresis

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Kopplungstechniken zur Elementspeziesanalytik

Dunemann

Begerow

1995

Kopplungstechniken Zur Elementspeziesanalytik

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Capillary zone electrophoresis with UV‐scanning detection for the analysis of metal species and their organic binding partners in biological systems

Theobald

Dunemann

1996

J. High Resol. Chromatogr.

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SummaryA capillary zone electrophoretic method with UV-scanning detection for the separation and identification of both free ligands and metal species is presented. The electrophoretic behavior of naturally occurring binding partners such as organic acids and amino acids was studied and compared with their metal-complexes. Copper(I1) complexes of citrate and amino acids showed decreased electrophoretic mobilities and altered UV spectra. The optimized method was validated on ultrafiltered cow's milk and human milk samples. In cow's milk six low molecular weight substances, including citrate, orotate, and hippurate, could be separated. Metal supplementation with Cu(II), Ni(II), or Zn(I1) decreased not only the citrate peak but also the orotate signal and in the case of copper(I1) supplementation a new signal for the copper(I1)-citrate complex appeared. In human milk samples various amino acids such as glutamate, phenylalanine, tyrosine, and tryptophan could be identified besides citrate. The electrophoretic mobilities and peak areas of the amino acids were also influenced by the metal supplementation.

show abstract

Electrophoretic techniques in element species analysis. Comparison between flow-through and flat-bed techniques

Cited by 7 publications

References 6 publications

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

Speciation of protein‐bound trace elements by gel electrophoresis and atomic spectrometry

Kopplungstechniken zur Elementspeziesanalytik

Capillary zone electrophoresis with UV‐scanning detection for the analysis of metal species and their organic binding partners in biological systems

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