Electrophoretic Technique: Capillary Zone Electrophoresis

Álvarez‐Rivera, Gerardo; Castro-Puyana, María

doi:10.1016/b978-0-12-814264-6.00016-5

Cited by 4 publications

(1 citation statement)

References 73 publications

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“…Capillary electrophoresis (CE) is a simple and fast separation technique based on the different migration times of species in the presence of an electrical field [12,13]. Compared to chromatographic methods, CE has been recognized as a viable alternative separation technique due to its ability to achieve complete separation of several compounds in a short analysis time, high separation efficiency and resolution, and minimal sample and reagent usage without the need for specific columns [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Analysis of naphthenic acids in produced water samples by capillary electrophoresis‐mass spectrometry

Pinheiro

Sako

Rodrigues

et al. 2023

J of Separation Science

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A capillary electrophoresis‐mass spectrometry method was used to analyze naphthenic acids in produced water samples. It was possible to detect cyclopentanecarboxylic, benzoic, cyclohexanebutyric, 1‐naphthoic, decanoic, 3,5‐dimethyladamantane‐1‐carboxylic, 9‐anthracenecarboxylic, and pentadecanoic acids within ca. 13 min using a buffer composed of 40 mmol/L ammonium hydroxide, 32 mmol/L acetic acid and 20% v/v isopropyl alcohol, pH 8.6. The proposed method showed good repeatability, with relative standard deviation (RSD) values of 6.6% for the sum of the peak areas and less than 2% for the analysis time. In the interday analysis, the RSD values for the sum of the peak areas and migration time were 10.3% and 10%, respectively. The developed method demonstrated linear behavior in the concentration range between 5 and 50 mg/L for benzoic, decanoic, 3,5‐dimethyladamantane‐1‐carboxylic and 9‐anthracenecarboxylic acids, and between 10 and 50 mg/L for cyclopentanecarboxylic, cyclohexanebutyric, 1‐ naphthoic, and pentadecanoic acids. The detection limits values ranged from 0.31 to 1.64 mg/L. Six produced water samples were analyzed and it was possible to identify and quantify cyclopentanecarboxylic, benzoic, cyclohexanebutyric, and decanoic acids. The concentrations varied between 4.8 and 98.9 mg/L, proving effective in the application of complex samples.

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Section: Introductionmentioning

confidence: 99%

Analysis of naphthenic acids in produced water samples by capillary electrophoresis‐mass spectrometry

Pinheiro

Sako

Rodrigues

et al. 2023

J of Separation Science

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Recent biopharmaceutical applications of capillary electrophoresis methods on recombinant DNA technology‐based products

et al. 2021

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Recent biopharmaceutical applications of capillary electrophoresis methods on recombinant DNA technology-based productsBiopharmaceuticals (recombinant technology-based products, vaccines, whole blood and blood components, gene therapy, cells, tissues, etc.,) are described as biological medical products produced from various living sources such as human, microbial, animal, and so on by manufacturing, extraction, or semi-synthesis. They are complex molecules having high molecular weights. For their safety and efficacy, their structural, clinical, physicochemical, and chemical features must be carefully controlled, and they must be well characterized by analytical techniques before the approval of the final product. Capillary electrophoresis (CE) having versatile modes can provide valuable safety and efficacy information, such as amino acid sequence, size variants (low and high molecular weight variants), charged variants (acidic and basic impurities), aggregates, N-linked glycosylation, and O-linked glycosylation. There are numerous applications of CE in the literature. In this review, the most significant and recent studies on the analysis of recombinant DNA technology-based products using different CE modes in the last ten years have been overviewed. It was seen that the researches mostly focus on the analysis of mAbs and IgG. In addition, in recent years, researchers have started to prefer CE combined mass spectrometry (MS) techniques to provide a more detailed characterization for protein and peptide fragments.

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Advances in detection technology for authentication of vegetable oils: A comprehensive review

Srivastava,

Pandey,

Singh

et al. 2024

Heliyon

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Electrophoretic Technique: Capillary Zone Electrophoresis

Cited by 4 publications

References 73 publications

Analysis of naphthenic acids in produced water samples by capillary electrophoresis‐mass spectrometry

Analysis of naphthenic acids in produced water samples by capillary electrophoresis‐mass spectrometry

Recent biopharmaceutical applications of capillary electrophoresis methods on recombinant DNA technology‐based products

Advances in detection technology for authentication of vegetable oils: A comprehensive review

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