Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil

Pereira, H. G.; Azeredo, Rosete S; Leite, Joana Pereira; Candeias, J. A. N.; Rácz, Maria Lucia; Linhares, Alexandre da Costa; Gabbay, Yvone Benchimol; Trabulsi, J. R.

doi:10.1017/s0022172400063919

Cited by 99 publications

(84 citation statements)

References 26 publications

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“…Cocirculation of different electropherotypes or replacement of one by another have been found in many parts of the world (Espejo et al 1980;Rodger et al 1981;Schnagl, Rodger & Holmes, 1981;Lourenco et al 1981;Flores et al 1982;Pereira et al 1983;Follett & Desselberger, 1983 a). At present it is not quite clear how these differences emerge and what their significance is.…”

Section: Discussionmentioning

confidence: 99%

“…The RNA of rotaviruses can be readily isolated and segments separated by electrophoresis on polyacrylamide gels (Kalica et al 1976(Kalica et al , 1978Espejo et al 1980;Schnagl, Rodger & Holmes, 1981;Rodger et al 1981;Lourenco et al 1981;Flores 210 E. A. C. FOLLETT AND OTHERS et al 1982;Pereira et al 1983;Follett & Desselberger, 1983a). RNA migration patterns ('electropherotypes', Rodger et al 1981, Schnagl, Rodger & Holmes, 1981; 'electrophoretypes', Thouless, Beards & Flewett, 1982) can thus be determined.…”

Section: Introductionmentioning

confidence: 99%

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Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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SUMMARYThe molecular epidemiology of rotavirus infections in Glasgow and the west of Scotland during 1981/82 and 1982/83 was investigated by electron microscopy, ELISA testing and RNA migration pattern analysis. In 1981/82, rotaviruses of both the 'long' and the 'short 'electropherotype (in different variants) co-circulated from the onset throughout the winter peak of the outbreak. Approximately 80 o of the children were infected during the first year of life. No differences in incidence were found between sexes. In 1982/83 the isolated rotaviruses were almost exclusively of the 'long' electropherotype (in different variants) and 360 of the children were infected beyond the first year of life. Rotaviruses of the 'long' electropherotype serologically were of subgroup ii and serotype 1 and those of the 'short' electropherotype of subgroup i and serotype 2.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Follett

Sanders

Beards

et al. 1984

J. Hyg.

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“…Considering the last population census carried out by Instituto Brasileiro de Geografia e Estatística (IBGE 2000), the sampling analyzed in relation to the infantile population was regarded as representative in both cities (95% confidence interval). All samples were primarily screened for rotavirus and adenovirus utilizing a combined enzyme immunoassay for Rotavirus A and adenovirus -EIARA - (Pereira et al 1985) and also by polyacrylamide gel electrophoresis -PAGE (Pereira et al 1983). Samples were also screened for calicivirus by RT-PCR using primers designed for the RNA polymerase region (Green et al 1995, Vinjé et al 1997.…”

Section: Methodsmentioning

confidence: 99%

Astrovirus infection in children living in the Central West region of Brazil

Santos¹,

Borges²,

Costa

et al. 2007

Mem. Inst. Oswaldo Cruz

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This study presents data regarding the circulation of astrovirus in Goiânia-GO and Brasília-DF. These viruses were detected in fecal samples from hospitalized children up to five years old with and without acute gastroenteritis. A total of 1244 fecal samples were collected in two periods, 1994 to 1996 (Brasília) and 1998, and were analyzed for viral RNA using the reverse transcription-polymerase chain reaction (RT-PCR)

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“…For other types of rotavirus, polyacrilamide gel electrophoresis (PAGE) was used according to Pereira et al (1983) and the gels were stained with silver die (Herring et al 1982). Fecal samples were first submitted to extraction by the phenol /chloroform method, starting with a 20% fecal suspension.…”

Section: Virological Examinationmentioning

confidence: 99%

Matched case-control study evaluating the frequency of the main agents associated with neonatal diarrhea in piglets

Lippke

Borowski²,

Marques

et al. 2011

Pesq. Vet. Bras.

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A case-control study was carried out in litters of 1 to 7-day-old piglets to identify the main infectious agents involved with neonatal diarrhea in pigs. Fecal samples (n=276) from piglets were collected on pig farms in the State of Rio Grande do Sul, Brazil, from May to September 2007. Litters with diarrhea were considered cases (n=129) and normal litters (n=147) controls. The samples were examined by latex agglutination test, PAGE, conventional isolating techniques, ELISA, PCR, and microscopic methods in order to detect rotavirus, bacterial pathogens (Escherichia coli, Clostridium perfringens type A and C, and Clostridium difficile), and parasites (Coccidian and Cryptosporidium spp.). Outbreaks of diarrhea were not observed during sampling. At least one agent was detected in fecal samples on 25 out of 28 farms (89.3%) and in 16 farms (57.1%) more than one agent was found. The main agents diagnosed were Coccidia (42.86%) and rotavirus (39.29%). The main agents identified in litters with diarrhea were Clostridium difficile (10.6%), Clostridium perfringens type A (8.8%) and rotavirus (7.5%); in control litters, Clostridium difficile (16.6%) and Coccidian (8.5%). Beta hemolytic Escherichia coli and Clostridium perfringens type C were not detected. When compared with controls, no agent was significantly associated with diarrhea in case litters. These findings stress the need for caution in the interpretation of laboratorial diagnosis of mild diarrhea in neonatal pigs, as the sole detection of an agent does not necessarily indicate that it is the cause of the problem. dos surtos e a diarréia, quando presente, apresentou-se leve. Pelo menos um agente foi identificado nas amostras fecais de 25 entre 28 granjas (89,3%) analisadas e em 16 granjas (57,1%) mais de um agente foi detectado. Os principais agentes encontrados nas granjas foram coccídeos (42,86%) e rotavírus (39,29%). Os principais agentes detectados nas leitegadas com diarreia foram Clostridium difficile (10,6%), Clostridium perfringens tipo A (8,8%) e rotavírus (7,5%). Por outro lado, nas leitegadas controle os agentes mais prevalentes foram Clostridium difficile (16,6%) e coccídeos (8,5%). E. coli Beta hemolítica e Clostridium perfringens tipo C não foram detectados. O presente estudo de caso-controle demonstrou que nenhum agente infeccioso esteve associado significativamente com diarreia (p>0.05). Esses achados reforçam a necessidade de que haja cuidado na interpretação de resultados de exames laboratoriais em materiais coletados de leitões com diarreia neonatal leve, pois a detecção isolada de um agente infeccioso não indica necessariamente que o mesmo seja a causa do problema.

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Electrophoretic study of the genome of human rotaviruses from Rio de Janeiro, São Paulo and Pará, Brazil

Cited by 99 publications

References 26 publications

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Molecular epidemiology of human rotaviruses: Analysis of outbreaks of acute gastroenteritis in Glasgow and the west of Scotland 1981/82 and 1982/83

Astrovirus infection in children living in the Central West region of Brazil

Matched case-control study evaluating the frequency of the main agents associated with neonatal diarrhea in piglets

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