Electrophoretic separation of the soluble proteins of different fractions of the sarcocyst of Sarcocystis fusiformis of buffalo (Bubalus bubalis)

Khulbe, D.C.; Kushwah, Ameeta; Kushwah, H. S.; Quadri, M. A.

doi:10.1016/0304-4017(89)90113-1

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2022

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“…Almost similar number of polypeptides has also been observed by Khulbe et al (1989) (1990) may be due to use of silver nitrate staining which is said to be more sensitive (Laemmli, 1970) in comparison to coomassie blue staining. The differences in the banding patterns compared to previous reports could be due to type of species or parasitic stage used for antigen or methodology used for preparing antigen.…”

Section: Resultssupporting

confidence: 56%

Immunoreactive polypeptide profiles of Sarcocystis fusiformis using hyperimmune and naturally infected serum

et al. 2022

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Immunoreactive polypeptide profiles study was made in the whole cyst lysate antigen of Sarcocystis fusiformis which was collected from buffaloes slaughtered at local slaughterhouse, Mathura, U.P. When whole cyst lysate antigen was subjected to SDS-PAGE, twelve major polypeptides (78, 66, 53, 50, 42, 39, 32, 29, 27, 24, 19 and 15kDa) were observed. On western blotting, out of twelve, six (32, 39, 42, 53, 66, 78kDa) and two polypeptides (53kDa and 66kDa) were found to be immunoreactive when probed with homologous hyperimmune and pooled known positive natural serum. No immunoreactivity was seen with pooled known negative natural serum. These two polypeptides (53kDa and 66kDa) bands can be explored for the development of sensitive and specific diagnostic assays.

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Section: Resultssupporting

confidence: 56%