Electrophoretic RNA genomic profiles of Brazilian Picobirnavirus (PBV) strains and molecular characterization of a PBV isolated from diarrheic calf

Abstract

Picobirnavirus (PBV) belongs to the family Picobirnaviridae. PBV are a group of emerging non-enveloped viruses, with a bisegmented double-stranded RNA genome that can infect a wide range of hosts. This study reports the occurrence of PBV in fecal samples from five Brazilian dairy cattle herds. From the 289 stool samples of individual calves analyzed by silver-stained polyacrylamide gel electrophoresis (ss-PAGE) the PBV was detected in 8.3 % (24/289), of which 10.2% (18/176) had diarrheic consistency. Of the 24 positive samples in ss-PAGE, 5 (20.8%) of them showed a small electrophoretic profile and 19 (79.2%) samples had large profile. From the 24 positives samples by ss-PAGE, 15 (62.5%) were successfully amplified (201 bp) using GI specific primers targeting the RdRp gene of PBV. The analysis of nucleotide identity matrix revealed that the bovine PBV strain identified in this study, showed the highest nucleotide identity (81%) with PBV strain detected in turkey (MD-2010/HM803965). This is the first nucleotide sequence of a bovine PBV strain in the American continent and the first detection of small genome profile of PBV-like strains in bovine hosts.

Detection of novel RNA viruses from free-living gorillas, Republic of the Congo: genetic diversity of picobirnaviruses

“…These viruses were first identified from human [34] and rat stools in 1988 from Brazil [35]. Since then, these viruses have been detected from various symptomatic and asymptomatic hosts, i.e., human [36], domestic animals (bovine/cows/calf [37], cat [38], chicken, dogs [39], mouse [40], dromedary [41], pigs [42], rats [39], rabbit [43], horse/foals [44], turkey [45], wild animals (fox/red fox) [46], jaguar, lion [47], monkey [48], wild rodent [40], orangutan [49], puma [47], rhesus macaques [1], seal lion [50], small carnivores [51], snakes [39], wolf, bats (mammals) [52], and also in waste water [53]. However, only partial sequences are available for many of these viruses.…”

Genetic diversity of bovine Picobirnavirus, Brazil

“…Picobirnavirus (PBV) is the unique genus of the family Picobirnaviridae, classified into two different species: Human picobirnavirus and Rabbit picobirnavirus [1,2]. It is a nonenveloped double-stranded segmented RNA virus (dsRNA) constituted of an icosahedral symmetry capsid with a diameter ranging from 35 to 40 nm that protects the two segments of the genomic dsRNA [3,4].…”

Evolving views on enteric viral infections of equines: an appraisal of key pathogens

“…With the advancement of molecular diagnostic techniques such as RT-PCR (Ganesh et al, 2012;Malik et al, 2013;Takiuchi et al, 2016) and qRT-PCR (Malik, unpublished data) for picobirnaviruses, they have been detected in faecal and respiratory samples from over 20 animal species including rodents, aves and large animals like rats, hamsters, guinea pigs, giant ant eater, dogs, pigs, bovine calves, buffalo calves, foals camels, snakes worldwide and exhibit vast genetic diversity (Ganesh et al, 2011;Malik et al, 2011;Smits et al, 2011;Gillman et al, 2013;Malik et al, 2013;Malik et al, 2014;Ng et al, 2014;Ribeiro et al, 2014;Woo et al, 2014, Verma et al, 2015Takiuchi et al, 2016). Despite the broad host range, pathogenicity of PBVs alone or as co-infecting agents remains unclear.…”