Electrophoretic resolution and comparison of brain proteins

Vaughan, WJ; Fliesler, Steven J.

doi:10.1111/j.1471-4159.1974.tb06948.x

Cited by 7 publications

(3 citation statements)

References 16 publications

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“…It is possible that the band at 47 000 which was missing in blinded rats also represents a myelin component. The absence of a band at about 45 000 has been observed in quaking mice [2], a mutation characterized by myelin deficiency in the central nervous system with preservation of axons [8].…”

Section: Discussionmentioning

confidence: 99%

“…The histological complexity of the central nervous system is a major difficulty to interpret descriptive surveys of brain proteins and the comparison of normal and pathological tissues with selective loss of cell constituents has provided a useful approach to the problem [ 1,2] . In this report we have utilized Wallerian degeneration of the rat optic nerves as an experimental model to study the polypeptide composition of axons and glial fibers.…”

Section: Introductionmentioning

confidence: 99%

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Protein differences associated with the loss of myelinated axons and fibrillary gliosis in rat optic nerves following Wallerian degeneration

Dahl¹,

Bignami²

1975

FEBS Letters

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Protein differences associated with the loss of myelinated axons and fibrillary gliosis in rat optic nerves following Wallerian degeneration

Dahl¹,

Bignami²

1975

FEBS Letters

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“…Gel densitometer scans were used to insure that the intensity of each band increased linearly with protein loaded onto the gel (36). Further details of the gel system and operational procedures are available elsewhere (36,37).…”

Section: Methodsmentioning

confidence: 99%

Electrophoretic Analysis of Brain Proteins from Young Adult and Aged Mice

Vaughan¹,

Calvin²

1977

Gerontology

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The purpose of this study was to electrophoretically examine proteins from mice of different ages to determine whether general changes occurred in protein levels during senescence and to relate the data to specific ageing hypotheses involving proteins. Proteins from whole brain, brain regions, soluble and ribosomal brain fractions, from young adult and senescent mice, were totally solubilized and subjected to polyacrylamide slab gel electrophoresis. The resulting gel patterns were then compared by examining the intensity of gel bands of the same migration distance. Positive controls were used to establish the high sensitivity of the electrophoretic method. For example, band intensity differences were observed when brain proteins from different species, different periods of growth and development, normal and mutant mice, and different brain regions were compared. Virtually all band intensities and, therefore, corresponding brain protein concentrations were unchanged when patterns from senescent mice were compared to patterns from younger adult mice. These results provide evidence against ageing hypotheses involving: (1) progressive programmed ageing; (2) protein cross-linking, and (3) a systematic shutdown of gene activity. The few, select, protein band changes that did occur provide evidence that (1) the volume of the brain vascular system decreases with age, and (2) the brain S-100 protein concentration increases with age.

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Biochemical and electrophoretic studies of cholinesterases in the muscular dysgenesis (mdg) mutant mouse

1979

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Acetyl-and butyrylcholinesterase activities were investigated in muscle, brain, blood plasma and erythrocyte membranes obtained from adult and fetal mice of different genotypes ( + I + , +lmdg, +I?, and mdglmdg) with respect to the muscular dysgenesis ( m d g ) mutant gene. The study revealed that both AChE and BChE are present in muscle, brain and plasma, whereas erythrocyte membranes exhibit only AChE activity. Significantly lower (P < 0.05) AChE activity was observed in thigh muscle of 20-day-old fetuses homozygous for the mutant gene, compared with muscles of wild ( + I + ) and unknown genotypes (+I?). The mutant (mdg/mdg) fetuses had a slightly significantly

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Electrophoretic resolution and comparison of brain proteins

Cited by 7 publications

References 16 publications

Protein differences associated with the loss of myelinated axons and fibrillary gliosis in rat optic nerves following Wallerian degeneration

Protein differences associated with the loss of myelinated axons and fibrillary gliosis in rat optic nerves following Wallerian degeneration

Electrophoretic Analysis of Brain Proteins from Young Adult and Aged Mice

Biochemical and electrophoretic studies of cholinesterases in the muscular dysgenesis (mdg) mutant mouse

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