Electrophoretic pattern of Linguatula serrata larva isolated goat mesenteric lymph node

Abstract

Linguatula serrata, one of the parasitic zoonoses, inhabits the canid and felid respiratory system. The parasite is tongue-shaped, lightly convex dorsally and flattened ventrally. Males measure 1.8-2 cm, while females measure 8-13 cm in length. Disease due to infection with this parasite in humans is more likely to cause pharyngitis, nausea and vomiting, sore and itchy throat, cough, phlegm and runny nose. Present study aimed to determine linguatula's larva somatic antigens in lymph nodes of infected goats and also reveal the major component of antigenic protein. To determine the electrophoretic pattern of L. serrata's larvae, 50 samples were taken from goat's referred to the slaughter house of Amol, Mazandaran, Iran. After performing SDS-PAGE on somatic antigens, 6 bands (19, 20, 36, 48, 75,100 KDa) were seen in which the 36, 48 and 75 KDa bands were more prominent. In conclusion, it is recommended to determine the most important antigenic protein of this parasite could be used an experimental model in infection up to determine the most significant component of this parasite's antigen and use of that in immunogenicity and detection of infection.

Comparative evaluation of somatic proteins of the nymphal stage of linguatula serrata isolated from goats and cattle in Northwest Iran

“…Out of these bands, the 36 and 48 kDa ones were more prominent. Our study results are incompatible with the findings of Youssefi et al (2014) who researched on electrophoretic pattern of somatic proteins of L. serrata nymph isolated from goat mesenteric lymph nodes and found out 6 protein bands (19,20,36,48,75, 100 kDa). However, our research scope and findings were different; we extended our focus from goat to cattle mesenteric lymph nodes and our results indicated 2 different bands of protein.…”

“…To date, the existing research has focused on the somatic proteins in isolated L. serrata nymph from mesenteric lymph nodes of goats (Youssefi et al, 2014). Therefore, the objective of this study was to compare the somatic proteins of the nymphal stage of L. serrata isolated from goats and cattle mesenteric lymph nodes.…”