Electrophoretic motion of a nanorod along the axis of a nanopore under a salt gradient

Joo, Sang Woo; Qian, Shizhi

doi:10.1016/j.jcis.2010.12.062

Cited by 10 publications

(7 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To uncover the mechanism of current change of our experiments, we developed a model based on the Poisson, Nernst−Planck, and Stokes equations to explain the phenomena. The model has been successfully applied to simulate the DNA translocation through a nanopore under various conditions, including different solution temperature, 34,35 electrolyte concentration gradients, 16,36 and functionalized pore surface. 37−39 In order to figure out the key factors contributing to the current blockage, the flow fields inside the nanopore with or without DNA are simulated by COMSOL (commercial Multiphysics software, Bandon, MA) based on the finite element method.…”

Section: ■ Methods and Materialsmentioning

confidence: 99%

Salt Gradient Improving Signal-to-Noise Ratio in Solid-State Nanopore

et al. 2017

View full text Add to dashboard Cite

As the single molecule detection tool, solid-state nanopores are being applied in more and more fields, such as medicine controlled delivery, ion conductance microscopes, nanosensors, and DNA sequencing. The critical information obtained from nanopores is the signal collected, which is the ionic block current caused by the molecules passing through the pores. However, the information collected is, in part, impeded by the relatively low signal-to-noise ratio of the current solid-state nanopore measurements. Here, we report that using a salt gradient across the nanopore could improve the signal-to-noise ratio when molecules translocate through SiN nanopore. Furthermore, we demonstrate that the improved signal-to-noise ratio is connected with not only the value of surface charge but also that of a salt gradient between cis and trans sides of the nanopore.

show abstract

Section: ■ Methods and Materialsmentioning

confidence: 99%

Salt Gradient Improving Signal-to-Noise Ratio in Solid-State Nanopore

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Several approaches have been verified to face this challenge . Among the various strategies examined, such as active controls by means of adding external forces through light irradiation and gate voltages as well as passive methods via changing viscosity , and temperature of liquid, a salt gradient approach was reported to be useful for manipulating the translocation dynamics through the induced self-built electric field that serves not only to slow down the motions of objects such as DNA − and nanoparticles − but also to raise the capture rates in the conduit. − Meanwhile, the mechanism is predicted to become ineffective in pores of size much larger than the Debye length since it relies on ion-selective transport across the membrane to induce ion concentration polarization via the profound influence of surface charges on the nanopore wall. − Despite the fact that such condition is common in nanopore sensing of relatively large particles and molecules such as viruses and amyloids, along with the fact that various intriguing phenomena have been found in resistive pulse sensing using submicrometer channels such as pore shape-dependent ion blockage characteristics, ,, deformations of soft particles, − and concentration-polarization-induced ionic current enhancements, , little experimental efforts have been devoted so far to assess the feasibility of the salt gradient approach for controlling the translocation dynamics of non-DNA objects in the non-ion-selective channels.…”

Section: Introductionmentioning

confidence: 99%

Salt Gradient Control of Translocation Dynamics in a Solid-State Nanopore

et al. 2021

View full text Add to dashboard Cite

Tuning capture rates and translocation time of analytes in solid-state nanopores are one of the major challenges for their use in detecting and analyzing individual nanoscale objects via ionic current measurements. Here, we report on the use of salt gradient for the fine control of capture-to-translocation dynamics in 300 nm sized SiN x nanopores. We demonstrated a decrease up to a factor of 3 in the electrophoretic speed of nanoparticles at the pore exit along with an over 3-fold increase in particle detection efficiency by subjecting a 5fold ion concentration difference across the dielectric membrane. The improvement in the sensor performance was elucidated to be a result of the salt-gradient-mediated electric field and electroosmotic flow asymmetry at nanochannel orifices. The present findings can be used to enhance nanopore sensing capability for detecting biomolecules such as amyloids and proteins.

show abstract

“…Numerous efforts have been made towards the electrophoresis analysis of entities in an unbounded medium [10][11][12][13][14] and a bounded one. [15][16][17][18][19][20][21][22][23] In the latter, a cylindrical channel [16][17][18][19][20][21][22] was often adopted to study the boundary effect on electrophoresis.…”

Section: Introductionmentioning

confidence: 99%

Electrophoresis of a soft sphere in a necked cylindrical nanopore

Tseng

Hsu

et al. 2013

Phys. Chem. Chem. Phys.

View full text Add to dashboard Cite

The influence of boundary shape on electrophoresis is modeled by considering a soft spherical particle comprising a positively charged rigid core and an uncharged membrane layer on the axis of a necked cylindrical pore with its throat positively charged. The presence of the throat makes the associated flow and electric fields nonuniform, yielding several interesting behaviors. In general, the reduction in the cross-section area of the pore intensifies the local electric field and, therefore, accelerates the particle. It also makes the ionic distribution nonuniform, and the electric field induced accelerates the particle. The maximum mobility occurs at the center of a throat, and the higher the charge density of the throat the larger the ratio of maximum mobility/mobility far away from the throat. This result is informative for the design of separation devices having variable cross sectional area.

show abstract

Electrophoretic motion of a nanorod along the axis of a nanopore under a salt gradient

Cited by 10 publications

References 67 publications

Salt Gradient Improving Signal-to-Noise Ratio in Solid-State Nanopore

Salt Gradient Improving Signal-to-Noise Ratio in Solid-State Nanopore

Salt Gradient Control of Translocation Dynamics in a Solid-State Nanopore

Electrophoresis of a soft sphere in a necked cylindrical nanopore

Contact Info

Product

Resources

About