Electrophoretic Light Scattering, Dynamic Light Scattering, and Turbidimetry Studies of the Effect of Polymer Concentration on Complex Formation between Polyelectrolyte and Oppositely Charged Mixed Micelles

Li, Yingjie; Dubin, Paul L.; Havel, Henry A.; Edwards, Shun L.; Dautzenberg, H.

doi:10.1021/ma00113a011

Cited by 42 publications

(48 citation statements)

References 17 publications

(27 reference statements)

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“…Static [28,30,49] and quasielastic [25,[27][28][29]49] light scattering especially have provided important information on the size and structure of polymermicelle complexes but soluble complexes can only be detected by QELS if their lifetime (residence time) is sufficiently long and the scattering intensity of the complexes is sufficiently large compared with those of the micelles and polymers from which they form.…”

Section: Introductionmentioning

confidence: 99%

Interactions of micelles with fluorescence-labeled polyelectrolytes

Morishima

Mizusaki

Yoshida

et al. 1999

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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Pyrene-labeled polyelectrolytes can be used to study the interaction between the polyelectrolyte and an oppositely charged micelle, if the micelle incorporates a fluorescence quencher. If the charge on the micelle is varied systematically, polymer-micelle complex formation can be observed at some well-defined micelle surface charge density. Applying a kinetic model to steady-state and time-dependent fluorescence quenching data, one can estimate the binding constant (K), association rate constant, and lifetime (residence time). K increases strongly with increase in micelle surface charge density (|) or decrease in ionic strength (v). These effects arise from the dependence of association rate constant and residence time on | and v. While these observations reflect the fundamental electrostatic nature of the interaction, it is also found that micelles preferentially bind to pyrene sites, so that complex formation results from the conjoint action of electrostatic and hydrophobic forces.

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Section: Introductionmentioning

confidence: 99%

Interactions of micelles with fluorescence-labeled polyelectrolytes

Morishima

Mizusaki

Yoshida

et al. 1999

Colloids and Surfaces A: Physicochemical and Engineering Aspect

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“…Static 20,22,41 and quasielastic 17,[19][20][21]41 light scattering especially have provided important insight into complex structure; nevertheless, they can detect the onset of complex formation only within certain limitations. Methods that depend on light scattered from the complex require a considerable change in particle size and/or mass relative to free polymers and free micelles, and in any event the scattering intensity increases only slightly when either polymer molecular weight 19,20 or concentration 22 is low.…”

Section: Introductionmentioning

confidence: 99%

Binding of Cationic Mixed Micelles to Pyrene-Labeled Poly((acrylamido)-2-methylpropanesulfonate)

et al. 1997

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The interaction between pyrene-labeled poly(acrylamido)-2-methylpropane sulfonate) (PyPAMPS) and mixed micelles of n-dodecylhexaoxyethylene glycol monoether/n-hexadecyltrimethylammonium chloride (C12E6/CTAC) was studied by turbidimetry, quasielastic light scattering (QELS), fluorescence quenching, and UV spectroscopy. The present report focuses on the effect of the pyrene label on the polymer-micelle interaction. With nonlabeled PAMPS, we observe by turbidity and QELS a critical mole fraction of ionic surfactant (Yc) corresponding to the onset of polyelectrolyte-micelle interaction. The same Yc is observed by turbidity and QELS for PyPAMPS. However, PyPAMPS shows a lower additional transition by the same methods, which we refer to as "Yc1" to distinguish it from "Yc2" which is seen for both PAMPS and PyPAMPS. Steady-state fluorescence, in the presence of a hydrophobic quencher (N,N-dimethylaniline) solubilized in the micelles, also shows a discontinuity at Yc1. Therefore, we conclude that Yc1 and Yc2 correspond to the binding of micelles to polymeric pyrene sites and AMPS sites, respectively. Analysis of UV spectra at varying Y demonstrates that the polymer-bound pyrene penetrates inside micelles and resides at or near the hydrophobic core. These results indicate preferential binding of micelles to pyrene binding sites; nevertheless, both Yc1 and Yc2 show a linear dependence on the square root of the ionic strength. This dependence suggests the dominant role of electrostatic forces, consistent with the observation that nonionic micelles will not bind to PyPAMPS. We conclude that conjoint hydrophobic and electrostatic effects determine the interaction between PyPAMPS and C12E6/ CTAC mixed micelles.

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“…Thus, the charge ratio between GFP:Tat and GFP:V was 1:2.31 and 2.24:1, respectively. The maximum complex formation between polyelectrolyte and the oppositely charged micelles at a 1:1 net charge ratio has been reported (Li et al, 1995). Considering the V peptide, the excess amount of V peptide in the mixtures might compete with V-GFP and V-Tat-GFP mixtures to bind with the receptor, resulting in lower GFP uptake efficiency than Tat-GFP mixtures.…”

Section: Discussionmentioning

confidence: 99%