Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates

Zanchet, Daniela; Micheel, Christine; Parak, Wolfgang J.; Gerion, Daniele; Alivisatos, A. Paul

doi:10.1021/nl005508e

Cited by 453 publications

(481 citation statements)

References 24 publications

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“…To obtain homogeneous samples of SM-featuring AuNP dimers, we use electrophoresis to purify gold particles conjugated preferentially to one DNA strand 17 and, after hybridization of complementary sequences, AuNP dimers linked by a single double strand 18 . In this study, we employ two 30 bases and 50 bases DNA sequences with a single ATTO647N molecule in the centre (respectively attached to amino-modified C and A bases, making this fabrication technique compatible with most water-soluble organic dyes).…”

Section: Dna-driven Nanoparticle Assemblymentioning

confidence: 99%

Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA

et al. 2012

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A photon interacts efficiently with an atom when its frequency corresponds exactly to the energy between two eigenstates. But at the nanoscale, homogeneous and inhomogeneous broadenings strongly hinder the ability of solid-state systems to absorb, scatter or emit light. By compensating the impedance mismatch between visible wavelengths and nanometre-sized objects, optical antennas can enhance light-matter interactions over a broad frequency range. Here we use a DnA template to introduce a single dye molecule in gold particle dimers that act as antennas for light with spontaneous emission rates enhanced by up to two orders of magnitude and single photon emission statistics. Quantitative agreement between measured rate enhancements and theoretical calculations indicate a nanometre control over the emitterparticle position while 10 billion copies of the target geometry are synthesized in parallel. optical antennas can thus tune efficiently the photo-physical properties of nano-objects by precisely engineering their electromagnetic environment.

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Section: Dna-driven Nanoparticle Assemblymentioning

confidence: 99%

Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA

et al. 2012

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“…Previous studies Poly-adenine-mediated formation of mDNA-AuNP conjugates G Yao et al have shown that the number of DNA strands on AuNPs is generally inversely related to their migration rate in gel electrophoresis. 24,38 We reasoned that the band (b1) located right above the band of nonfunctionalized AuNPs (b0) corresponded to mDNA-AuNPs, given that the migration of mDNA-AuNPs should be least retarded by the attached single DNA strand. The conjugate in b1 was recovered via electroelution.…”

Section: Resultsmentioning

confidence: 99%

“…4,9,10,[24][25][26][27][28][29][30][31][32][33] In a straightforward approach, AuNPs are titrated with DNA of different ratios, and then, the resulting conjugates are purified using gel electrophoresis to obtain mDNA-AuNPs. 10,24,32 The yield of this approach is usually very low. Improvement of the yield was realized using special chemical modification and/or double-stranded DNAs.…”

Section: Introductionmentioning

confidence: 99%

Clicking DNA to gold nanoparticles: poly-adenine-mediated formation of monovalent DNA-gold nanoparticle conjugates with nearly quantitative yield

et al. 2015

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Monovalent DNA-gold nanoparticle (mDNA-AuNP) conjugates hold great promise for widespread applications, especially the construction of well-defined, molecule-like nanosystems. Previously reported methods all rely on the use of thiolated DNA to functionalize AuNPs, resulting in relatively low yields. Here, we report a facile method to rapidly prepare mDNA-AuNPs using a poly-adenine (polyA)-mediated approach. As polyA can selectively bind to AuNPs with high controllability of the surface density of DNA, we can use a DNA strand with a sufficiently long polyA to wrap around the surface of an individual AuNP, preventing further the adsorption of additional strands. Based on this observation, we obtained mDNA-AuNPs with a nearly quantitative yield of~90% using 80 As, as confirmed by both gel electrophoresis and transmission electron microscope observation. The yields of mDNA-AuNPs were insensitive to the stoichiometric ratio between DNA and AuNPs, suggesting the click chemistry-like nature of this polyA-mediated reaction. mDNA-AuNPs exhibited rapid kinetics and high efficiency for sequence-specific hybridization. More importantly, we demonstrated that AuNPs of fixed valences could form well-defined heterogenous oligomeric nanostructures with precise, atom-like control.

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“…By choosing gold nanoparticles as model materials for the assembly, we have demonstrated the successful preparation of 1D gold nanoparticle arrays with lengths up to 4 m [10]. It has been previously shown that gold nanoparticles can be assembled into small, discrete structures [1,16,12,24] through hybridizing mono-DNA-modified gold particles with a DNA template. However, creating a gold nanoparticle array containing hundreds of nanoparticles does not seem to be an easy matter because of difficulties with the availability of long, single-stranded DNA templates.…”

Section: Dna Encoded One-dimensional Array Of Nanogoldmentioning

confidence: 99%

“…7). Also, gold nanoparticles can be modified with a thiolated single-stranded oligonucleotide, and mono-DNA-modified particles can be isolated simply by agarose gel electrohporesis using a protocol developed by Alivisatos et al [1,16,12,24]. After combining the mono-DNA-modified gold particles with the rolling-circle-synthesized long DNA template, 1D gold nanoparticle linear arrays with lengths up to several micrometers can be obtained.…”

Section: Dna Encoded One-dimensional Array Of Nanogoldmentioning

confidence: 99%

A Fresh Look at DNA Nanotechnology

Deng

Chen

Tian

et al.

Natural Computing Series

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Synthetic DNA structures for nanotechnological applications have experienced substantial success during the past decades benefiting from Seeman and his coworkers' pioneering work. In the last few years, some new branches have been emerging in this field. This review will summarize some recent progress in the authors' group. Two-Dimensional DNA Triangle Arrays Designed with a Tensegrity StrategyForming crystalline DNA lattices in one, two and even three dimensions has long been a hot topic of DNA nanotechnology. These artificially designed lattices are the basis for a variety of applications. The first success with a 2D DNA lattice was achieved in [22] with building blocks of double-crossover (DX) DNA molecules. Following that, rhombus motifs, triple-crossover molecules, and a cross motif were also constructed from branched four-arm Holliday junctions [19]. Here we present a tensegrity strategy for the construction of well-structured DNA triangle molecules [14]. A DNA triangle consists of three vertices (DNA four-arm junctions) and three sides (DNA duplexes). Although individual four-arm junctions are flexible, the rigidity of the three duplex edges restricts the freedom of the component four-arm junctions and only triangles can form. The shape of such a triangle is fully defined by the lengths of the three edges. By rational use of sticky-end cohesion, we have successfully assembled triangle arrays in one and two dimensions. Fig. 1 shows the design of some such triangle arrays and some atomic force microscope (AFM) images of such triangle arrays.

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Electrophoretic Isolation of Discrete Au Nanocrystal/DNA Conjugates

Cited by 453 publications

References 24 publications

Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA

Accelerated single photon emission from dye molecule-driven nanoantennas assembled on DNA

Clicking DNA to gold nanoparticles: poly-adenine-mediated formation of monovalent DNA-gold nanoparticle conjugates with nearly quantitative yield

A Fresh Look at DNA Nanotechnology

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