Electrophoretic Identification of Casein in Various Types of Milk

Yelubaeva, Makhpal Yelubaevna; Buralkhiev, Batyrkhan Azimkhanovich; Serikbayeva, Assiya; Narmuratova, Mirash Hudretovna; Kenenbay, Shynar

doi:10.3844/ojbsci.2017.348.352

Cited by 6 publications

(6 citation statements)

References 7 publications

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“…2B at the three different pH studied. The molecular weight of α-caseins, β-casein, and k-casein was observed to be about 28 kDa, 26 kDa, and 22 kDa, respectively, at each pH as expected and reported earlier (Yelubaeva et al, 2017;Kappeler et al, 1999). It was also clearly seen that the bands which correspond to whey protein in camel skimmed milk are decreased or disappeared in casein protein, depicting the relative purity of the isolated casein protein.…”

Section: Solubility and Sds Page Analysis Of Camel Milk Casein Proteinsupporting

confidence: 86%

Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells

Mittal¹,

Mahala²,

Krishna³

et al. 2022

Biocell

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Sorafenib, a multikinase inhibitor used for the treatment of hepatocellular carcinoma, is limited by its low oral bioavailability. To overcome this drawback, we have developed novel camel milk casein-derived nanoparticles as a drug delivery system. Camel milk casein is not only biocompatible on oral administration but is actually a dietary protein of pharmaceutical relevance. Casein is used because of its amphiphilic nature, self-assembling property, ability to show sustained release, and capability of encapsulating both hydrophilic and hydrophobic drugs. In this study, camel milk casein nanoparticles loaded with sorafenib were developed and characterized. Characterization of casein nanoparticles was done by dynamic light scattering (DLS), zeta potential analysis, scanning light microscopy (SEM), and FTIR. The drug content in nanoparticle and drug-protein binding studies were conducted by UV spectroscopy. The cytotoxicity and cellular uptake efficiency studies were performed in HepG2 cell lines. It was observed that the cytotoxic effect of sorafenib loaded camel milk casein nanoparticles was more than free sorafenib in HepG2 cells. This work suggests camel milk casein as a suitable drug delivery molecule for sorafenib. In the future, it may also be used in enhancing the efficacy and specific distribution of other water-insoluble anticancer drugs.

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Section: Solubility and Sds Page Analysis Of Camel Milk Casein Proteinsupporting

confidence: 86%

Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells

Mittal¹,

Mahala²,

Krishna³

et al. 2022

Biocell

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“…Unfermented dromedary milk protein bands ranged from 15 to 100 kDa, according to Yelubaeva et al (2017), whereas most protein bands in fermented dromedary milk were between 10 and 75 kDa, which is consistent with our findings. In the fermented dromedary milk sample, more protein was seen below 15 kDa but was absent in the unfermented sample.…”

Section: Antidiabetic and Ace Inhibitory Effects Of Fermented Camel M...supporting

confidence: 92%

“…Yelubaeva, Buralkhiev, Serikbayeva, Narmuratova, and Kenenbay (2017) compared the protein profiles of kumiss and shubat made from dromedary milk to those of other milk samples. Unfermented dromedary milk protein bands ranged from 15 to 100 kDa, according to Yelubaeva et al (2017), whereas most protein bands in fermented dromedary milk were between 10 and 75 kDa, which is consistent with our findings. In the fermented dromedary milk sample, more protein was seen below 15 kDa but was absent in the unfermented sample.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, L. helveticus showed an increased number of unique protein bands ranging from 9 to 17 kDa after 14 days of fermentation, which is similar to our findings. Yelubaeva, Buralkhiev, Serikbayeva, Narmuratova, and Kenenbay (2017) compared the protein profiles of kumiss and shubat made from dromedary milk to those of other milk samples. Unfermented dromedary milk protein bands ranged from 15 to 100 kDa, according to Yelubaeva et al (2017), whereas most protein bands in fermented dromedary milk were between 10 and 75 kDa, which is consistent with our findings.…”

Section: Resultsmentioning

confidence: 99%

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Exploring the potential of Lacticaseibacillus paracasei M11 on antidiabetic, anti‐inflammatory, and ACE inhibitory effects of fermented dromedary camel milk ( Camelus dromedaries ) and the release of antidiabetic and anti‐hypertensive peptides

Shukla¹,

Sakure

Pipaliya³

et al. 2022

Journal of Food Biochemistry

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The goal of this investigation was to find antidiabetic peptides and inhibit angiotensin converting enzyme (ACE) in Lacticaseibacillus paracasei (M11) fermented dromedary camel milk (Camelus dromedaries). According to the findings, the rate of antidiabetic activity increased along with the incubation periods and reached its peak after 48 hr of fermentation. The inhibitions of α‐amylase, α‐glucosidase, and lipase were 80.75, 59.62, and 65.46%, respectively. The inhibitory activity of ACE was 78.33%, and the proteolytic activity was 8.90 mg/mL. M11 at 0.25 mg/mL effectively suppressed LPS‐induced pro‐inflammatory cytokines and their mediators such as NO, TNF‐α, IL‐6, and IL‐1β in RAW 264.7 cells. The rate of inoculum in the optimization phase was 1.5–2.5%, and the greatest proteolytic activity was observed after 48 hr of fermentation. The investigation of the above property in the ultrafiltered fermented milk exhibited the highest antidiabetic and ACE inhibition activities in the 3 kDa than 10 kDa fractions. The molecular weight was determined employing SDS‐PAGE, and the six‐peptide sequences were identified using 2D gel electrophoresis. Due to its high proteolytic activity, the L. paracasei strain has been reported to be useful in the production of ACE‐inhibitory and antidiabetic peptides. Amino acid sequences such from ɑ1, ɑ2, and β‐caseins have been identified within fermented camel milk by searching on online databases, including BIOPEP (for antidiabetic peptides) and AHTPDB (for hypertension peptides) to validate the antidiabetic and ACE‐inhibitory actions of several peptides. Practical applications The study aims to identify antidiabetic peptides and inhibit ACE in dromedary camel milk fermented with Lacticaseibacillus paracasei M11. Maximum antidiabetic and ACE‐inhibitory actions of the fermented camel milk were observed in 3 kDa permeate fractions. Fermented camel milk significantly reduced the excessive TNF‐α, IL‐6, and IL‐1β production in LPS‐activated RAW 264.7 cells. RP‐LC/MS was used to identify 6 bioactive peptides from dromedary fermented camel milk. This fermented camel milk could be used for the management of hypertension and diabetic related problems.

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“…In previous decades, sending mares’ milk to the city was problematic as it could be kept fresh for only 1 hr before it would become sour. In contrast, milk powder, after dilution with water, returns to its original state (Yelubaeva et al., 2017).…”

Section: Findings and Discussionmentioning

confidence: 99%

Understanding the traditional mares’ milk industry’s transformation into a creative industry: Empirical evidence from Kazakhstan

et al. 2021

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The purpose of this paper is to explore the core forces and drivers of the creative transformation of a traditional industry through the assessment of the mares’ milk industry (MMI). Using an exploratory single case study, this research reveals the main driver mechanisms and challenges of the creative transformation of a traditional industry using the case of the MMI in Kazakhstan. Grounded theory, along with in‐depth interviews of 36 MMI business staff in Kazakhstan, has been used in this study. The research identifies the following four key driving factors for transforming a commodity‐based traditional industry into a cultural creative emerging industry: National demand, ingenuity and creativity, affordance (support), and actors (attitudes, skills, and characteristics). This study also explores challenges, such as administrative negligence, the threat of losing authenticity, poor industry cooperation, and cultural branding. A sustainable transformation into a creative MMI requires the government to give its full attention to implementing existing‐related policies, emphasizing the role of authenticity, stressing the importance of industry cooperation, and promoting iconic branding. The study findings will help policy makers, administrators, and related stakeholders to understand the transformation of the MMI when developing related policy, strategy, and sustainable development. The findings can also serve as guidelines for other traditional agricultural sectors that are undergoing transformation into creative industries.

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Electrophoretic Identification of Casein in Various Types of Milk

Cited by 6 publications

References 7 publications

Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells

Calcium chloride linked camel milk derived casein nanoparticles for the delivery of sorafenib in hepatocarcinoma cells

Exploring the potential of Lacticaseibacillus paracasei M11 on antidiabetic, anti‐inflammatory, and ACE inhibitory effects of fermented dromedary camel milk ( Camelus dromedaries ) and the release of antidiabetic and anti‐hypertensive peptides

Understanding the traditional mares’ milk industry’s transformation into a creative industry: Empirical evidence from Kazakhstan

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