Electrophoretic and Biochemical Characterization of Tetragonisca weyrauchi (Hymenoptera, Apidae) Stingless Bees Esterases

Ronqui, Ludimilla; Galhardo, Douglas; Lisboa, Francisco Tarcísio; Rúvolo-Takasusuki, Maria Cláudia Colla; Toledo, Vagner de Alencar Arnaut de

doi:10.18188/1983-1471/sap.v15n1p70-75

Cited by 2 publications

(2 citation statements)

References 7 publications

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“…(Vanhaecke et al, 2012). Ronqui, Galhardo, Lisboa, Ruvolo-Takasusuki, and Toledo (2016) states that isoenzymes can provide important information of species differentiation. Nei's genetic identity values (1978) found in this study for the populations from Ivaí and Pirapó Rivers are particular to co-specific populations.…”

Section: Genetic Identitymentioning

confidence: 99%

Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil

Vianna,

Azevedo,

Moreira

et al. 2023

Acta Sci. Biol. Sci.

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Species of the genus Pimelodella (Eigenmann & Eigenmann, 1988) present many similar morphological characteristics, which makes taxonomic identification difficult. Some species present morphological distinction and reproduce normally, while others present reproductive isolation without morphological distinction. Taking that into consideration, we used starch-gel electrophoresis of enzymes to compare two species of the genus Pimelodella collected in Paraná, Ivaí and Pirapó rivers of the Upper Paraná River basin. We analyzed the liver and muscle tissue of 82 Pimelodella avanhandavae and 29 Pimelodella gracilis individuals, which revealed few genetic differences between species. In addition, we carried out an experimental design based on the separation of four populations: P. avanhandavae, Parana River (n = 37); P. avanhandavae, Ivaí River (n = 6); P. gracilis, Ivaí River (n = 29); P. avanhandavae, Pirapó River (n = 39). The high proportions of polymorphic loci as well as the expected heterozygosity suggest that both species have high genetic variability. Nei’s genetic identity values and Wright's fixation indices revealed a large genetic proximity between the samples collected in Ivaí and Pirapó rivers, regardless of the species analyzed. The presence of fixed alleles at one hundred percent for two loci showed that P. avanhandavae individuals collected in the Parana River are genetically distant from all remaining populations. Data indicated that P. avanhandavae, collected in this river, constitutes a distinguished species from those collected in Ivaí and Pirapó rivers. Therefore, we suggest, that more studies with molecular markers be carried out with the genus, as well as a review of the diagnostic morphological characteristics used to separate P. avanhandavae from P. gracilis.

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Section: Genetic Identitymentioning

confidence: 99%

Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil

Vianna,

Azevedo,

Moreira

et al. 2023

Acta Sci. Biol. Sci.

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“…T. angustula is the most well-known species and has great diversity of nesting, which provides the species with high resilience in urban areas of many Brazilian states (Moure et al 2007;Camargo et al 2013). Furthermore, this tiny bee, known commonly as 'Jata ı', is broadly used in meliponiculture due to its easy management and large volume of studies related to its bionomy (Pirani and Cortopassi-Laurino 1993;Nogueira-Neto 1997;Oliveira et al 2004;Ronqui et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Botanical origin and seasonal variation in pollen collected by Tetragonisca weyrauchi (Apidae: Meliponini) in an urban area of Rio Branco, State of Acre, Brazil

et al. 2023

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The botanical origin and seasonal variation of pollen collected by Tetragonisca weyrauchi were studied by means of palynology (pollen identification). The study was carried out between August 2016 and July 2017 with a hive introduced in a house garden in an urban area (09 56 0 38.7 00 S; 67 52 0 42.9 00 W) near the campus of the Federal University of Acre (UFAC). In total, we identified 87 pollen types from 31 botanical families, with the most representative ones being: Alismataceae, Amaranthaceae, Arecaceae, Boraginaceae, Cyperaceae, Euphorbiaceae, Fabaceae and Urticaceae, all displaying percentages above 10%. The pollen types Alternanthera (Amaranthaceae), Cecropia (Urticaceae) and Cordia sellowiana (Boraginaceae) obtained relative abundances above 90% and are therefore considered events of temporary specialization. The lowest diversity and evenness values were found for July (H 0 ¼ 0.127; J 0 ¼ 0.071) and August (H 0 ¼ 0.070; J 0 ¼ 0.043), and the highest values for October (H 0 ¼ 2.025; J 0 ¼ 0.715) and April (H 0 ¼ 1.941; J 0 ¼ 0.611), thus not suggesting relationships with the rainy (November-April) or dry (May-October) periods. Furthermore, there was no preference by the bees for morphological characteristics of the pollen such as size, exine texture or aperture number. However, the most representative grains are among the small and medium size, reinforcing the hypothesis that this bee species prefers small flowers available in dense inflorescences.

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Electrophoretic and Biochemical Characterization of Tetragonisca weyrauchi (Hymenoptera, Apidae) Stingless Bees Esterases

Cited by 2 publications

References 7 publications

Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil

Enzymatic distance in two species of the genus Pimelodella (Siluriformes, Heptateridae) from the Upper Paraná River basin, Paraná State, Brazil

Botanical origin and seasonal variation in pollen collected by Tetragonisca weyrauchi (Apidae: Meliponini) in an urban area of Rio Branco, State of Acre, Brazil

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