Electrophoresis Using Pretreatment of Serum with Neuraminidase and Affinity Electrophoresis in the Evaluation of ALP Isoenzymes

Veterinary Science

“…Several methods have been proposed to differentiate the isoforms of canine ALP; however, there is no gold standard. Samples may or may not be pretreated with Vibrio cholerae neuraminidase, and affinity techniques may be used, incorporating wheat-germ lectin (WGL) into the migration gel (Chamberlain et al 1992;Bottoni et al 2003). Electrophoretic methods are also used, employing various support media, including cellulose acetate, agarose, and polyacrylamide.…”