Electrophoresis-tutor: an image-based personal computer program that teaches clinical interpretation of protein electrophoresis patterns of serum, urine, and cerebrospinal fluid

Astion, Michael L.; Rank, Joseph; Wener, Mark H.; Torvik, P; Schneider, J B; Killingsworth, L M

doi:10.1093/clinchem/41.9.1328

Cited by 7 publications

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“…Pemeriksaan elektroforesis protein berguna untuk mendiagnosis penyakit hati, kekurangan protein, kelainan hematologis, kegagalan ginjal dan penyakit gastrointestinal. [1][2][3][4] Keseluruhan (total) protein dalam darah terdiri dari albumin dan globulin. Proses elektroforesis mampu memisahkan bagian fraksi molekul protein dengan menggunakan medan listrik, sehingga terpisah beberapa fraksi molekul protein secara lebih rinci (detail) dan dapat pula mengukur kadar masing-masing bagian protein tersebut.…”

Section: Pendahuluanunclassified

“…1,7 Pemeriksaan elektroforesis protein serum menggunakan metode elektroforesis gel agarose (Sebia) dapat memisahkan bagian protein menjadi enam (6) bagian yaitu Albumin, alfa-1 globulin, alfa-2 globulin, beta-1 globulin, beta-2 globulin dan gamma globulin. [1][2][3][4][5][6] Albumin memiliki persentase 50% dari jumlah protein keseluruhan. Albumin membantu menjaga tekanan onkotik koloid dan berfungsi sebagai protein pengangkut berbagai jenis hormon dan obat-obatan.…”

Section: Pendahuluanunclassified

“…Masing-masing bagian protein plasma tersebut memiliki fungsi faali sebagai antibodi yang berfungsi menetralkan antigen yang khas (spesifik)-nya. 2,4,6 Nilai normal periksaan elektroforesis protein serum sesuai dengan metode elektroforesis gel agarose (SEBIA) adalah sebagai berikut: 4 Albumin 60,0-71,0% atau 4,3-5,1 g/dL Alfa-1 globulin 1,4-2,7% atau 0,10-0,20 g/dL Alfa-2 globulin 7,0-11,0% atau 0,5-0,8 g/dL Beta-1 globulin 6,0-9,0% atau 0,4-0,6 g/dL Beta-2 globulin 2,0-5,0% atau 0,1-0,4 g/dL Gamma globulin 8,0-16,0 % atau 0,6-1,20 g/dL Penafsiran (interpretasi) hasil memeriksa elektroforesis memerlukan ketelitian dan keterangan (informasi) keadaan klinis pasien, karena terdapat faktor penimbrungan yang dapat memengaruhi hasil memeriksa elektroforesis. Di tahap praperalatan (instrumentasi), menentukan kadar dalam (intra assay) dan pascaperalatan (post-instrumentasi), maupun keadaan (konstitusi) pasien, pengobatan yang diberikan kepada pasien.…”

Section: Sub Bagian Kimia Klinik Bagian Patologi Klinik Rumah Sakitunclassified

“…Hasil meneliti ini didapatkan pola elektroforesis protein serum tak wajar (abnormal) yang dapat terjadi pada peradangan (inflamasi), sirosis, sindroma nefrotik dan gamopati monoklonal, walaupun kadar protein keseluruhan (total) masih dalam batas wajar. [1][2][3][4]9 Di beberapa data ditemukan ketidaksesuaian antara pola elektroforesis dengan diagnosis klinis pasien, hal ini disebabkan karena pemeriksaan elektroforesis memiliki faktor penimbrungan (interferensi). Di tahap penentuan kadar dalam (intra assay), bahan periksa yang hemolisis akan menyebabkan peningkatan gelombang beta-1 globulin atau alfa-2 globulin.…”

Section: Pembahasanunclassified

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Elektroforesis Protein Serum Pasien Dengan Kadar Protein Normal

Rostini¹,

Rita²

2018

Indonesian J. Clin. Pathol. Med. Lab.

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Serum protein electrophoresis pattern can assist in diagnosis of liver disease, hematological disorders, renal disorders andgastrointestinal disease. Measurement of total protein level in the serum cannot detect any disorders in patient with normal limit ofserum total protein level. The aim of this study; was to evaluate the serum protein electrophoresis pattern in patient with normal limitsof serum protein level. This research was carried out by descriptive retrospective study using the electrophoresis data from patients’medical record at the Clinical Pathology Department, Dr. Hasan Sadikin General Hospital Bandung. The data of serum electrophoresis (bySebia gel electrophoresis) were grouped based on disease or disorders, and confirmed with the diagnosis derived from patient’s medicalrecord. Inclusion criteria of samples if ; the electrophoresis data were available, serum total protein level within normal limits (6.4–8.3mg/dL), and the data of electrophoresis taken from medical record were taken from August 2006 until August 2008. The result foundso far was, there were 240 data of electrophoresis from patients with serum protein level within normal limits (6.4–8.3 mg/dL). theinterpretation of electrophoresis consist of: 1) inflammation (149 patients; 62.2% ; sensitivity 83.7%, specificity 86,5%) 2) Cirrhosis(46 patients ; 19.2% ; sensitivity 87.5% ; specificity 88.4%) 3) Nephritic syndrome (15 patients ; 6.2%; sensitivity 53%; specificity96.9% 4) Monoclonal gammophaty (15 patients(6.2% ; sensitivity 80% ; specificity 98.7%) 5) Normal pattern in 15 patient (6.2%).This study found abnormal serum protein electrophoresis pattern in the condition of inflammation, Cirrhosis, Nephritic Syndrome, andMonoclonal gammophaty. It can be concluded that many disorders could be detected in patient with serum protein level within normallimits such as: inflammation, cirrhosis, nephritis syndrome and monoclonal gammophaty by abnormal electrophoresis pattern

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Section: Pendahuluanunclassified

Section: Sub Bagian Kimia Klinik Bagian Patologi Klinik Rumah Sakitunclassified

Section: Pembahasanunclassified

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Elektroforesis Protein Serum Pasien Dengan Kadar Protein Normal

Rostini¹,

Rita²

2018

Indonesian J. Clin. Pathol. Med. Lab.

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Teaching the Clinical Interpretation of Peripheral Blood Smears to a Second-Year Medical School Class Using the PeripheralBlood-Tutor Computer Program

et al. 1998

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The interpretation of peripheral blood smears has an important role in the diagnosis of hematologic diseases and is, therefore, part of the education of physicians and technologists. We describe a computer program, PeripheralBlood-Tutor (Lippincott-Raven, Philadelphia, Pa), that teaches the morphologic features of normal and abnormal peripheral blood smears; we also describe the evaluation of the effectiveness of the program in 133 second-year medical students who were required to use the program in their hematology course. The version of the PeripheralBlood-Tutor used in the study had 2 distinct but equivalent 20-question examinations; one examination, the pretest, was taken before the students viewed the contents of the program, and the other examination, the posttest, was taken after completing the program. The mean score on the pretest was 61% (SD, 14%), the mean on the posttest was 91% (SD, 10%), and the improvement was significant. In addition, 4 questions about peripheral blood smears, which were based on printed images, were administered at the end of the hematology course. The students scored an average of 2.75 (SD, 0.86), and a positive correlation was found between these scores and the scores on the Tutor posttest. The results of the study suggest that PeripheralBlood-Tutor is feasible to implement, and it helps students learn to interpret peripheral blood smears. The use of PeripheralBlood-Tutor is now a requirement in the medical school curriculum, the medical technology program, and the pathology residency at the University of Washington, Seattle.

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Teaching the microscopic examination of urine sediment to second year medical students using the Urinalysis-Tutor computer program

Phillips¹,

Henderson²,

Mandel

et al. 1998

Clinical Chemistry

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The microscopic examination of urine sediment is a common diagnostic tool taught to medical students, medical technologists, and others. The urine microscopic exam is difficult to teach because supervised instruction and textbook-based teaching suffer from numerous drawbacks. Here, we describe Urinalysis-Tutor, a computer program that uses digitized microscope images and computer-based teaching techniques to systematically teach the urine microscopic exam. In addition, we report the results of a 2-year study that evaluated the effectiveness of the program in 314 second year medical students who were required to use the program. The program contained two, 20-question exams. In the first year of the study (1996), one of the exams was chosen as the pretest and the other as the posttest; the pretest had to be completed before the students viewed the contents of the program, and the posttest was taken after finishing the tutorial. In 1997, the order of the two exams was reversed. In 1996, 159 students completed the study. The mean pretest score was 34% (SD, 14%), the mean posttest score was 71% (SD, 13%), and the improvement was significant (P <0.001, paired t-test). In 1997, 155 students participated. The mean pretest score was 41% (SD, 11%), the mean posttest score was 71% (SD, 13%), and the improvement was significant (P <0.001, paired t-test). The study shows that Urinalysis-Tutor helps medical students learn to interpret the microscopic appearance of urine sediment and that it is feasible to implement this tutorial in a medical school class.

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Electrophoresis-tutor: an image-based personal computer program that teaches clinical interpretation of protein electrophoresis patterns of serum, urine, and cerebrospinal fluid

Cited by 7 publications

References 4 publications

Elektroforesis Protein Serum Pasien Dengan Kadar Protein Normal

Elektroforesis Protein Serum Pasien Dengan Kadar Protein Normal

Teaching the Clinical Interpretation of Peripheral Blood Smears to a Second-Year Medical School Class Using the PeripheralBlood-Tutor Computer Program

Teaching the microscopic examination of urine sediment to second year medical students using the Urinalysis-Tutor computer program

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