Electrophoresis on Polyacrylamide Gels of Protein and Polysaccharide Fractions from Mycobacterium Tuberculosis^1,2,3

Mycobacterial antigens: a review of their isolation, chemistry, and immunological properties.

“…By this method, extremely h resolution is possible. Affronti et al first applied acrylamide gel electrophoresis to the fractionation of mycobacterial antigens (4). Unheated culture filtrate of M. tuberculosis and Seibert fractions were studied, and Rf values for major components were determined.…”

“…Other side chains contain principally mannose shown. If the D-galactose residues are in the furanose form, the linkage should be (1)(2)(3)(4)(5). Reproduced with permission of the Journal ofBiochemistry.…”

Electrophoresis

“…Microorganisms. Substances isolated from microbes include toxins and toxoids from clostridia (871,2336), staphylococci (1040), corynebacteria (1766,2034,2035), and pertussis vaccine (1134); Vi antigens (577,1148,1261,2211) and antigens from streptococci (1647,1748,2418), mycobacteria (16,1086,1623,1811), Pasteurella (538,2239), Rickettsia (1806), and Erysipelothrix (2261); allergens from Brucella (254); hemolysins from streptococci (757), staphylococci (1108,1133), and Listeria (758); proteins (559,651,1823,2319,2363) and colicins (Í4O8) from coliform organisms; nucleotide-bound peptides (118), cell wall components (1006), and enzymes (350) from mycobacteria; and antibodies from Serratia (1791). Methods have been suggested for differentiating the pleuropneumonialike organisms by electrophoresis (688); the effects on cell mobility of ionizing radiation and pH (721), of antibody binding (908), and of drugs ( 713) have been studied.…”

Separation of Mycobacterial Soluble Antigens by Isoelectric Focusing

“…The techniques utilized in attempting to isolate and purify mycobacterial antigens have varied and include ammonium sulfate precipitation (13,16), acid and alcohol precipitation (15), organic solvent extraction (2,6), disc electrophoresis (1,9), column chromatography (8,11), ultrafiltration (3,5), and ultracentrifugation (4). Chemical methods of extraction and fractionation usually result in an incomplete separation of antigens; in addition, some proteins may be denatured during the fractionation process.…”