Electrophoresis of tissue glycosaminoglycans as an aid in the diagnosis of mesotheliomas

Waxler, Beverly; Eisenstein, Reuben; Battifora, Hector

doi:10.1002/1097-0142(197907)44:1<221::aid-cncr2820440136>3.0.co;2-o

Cited by 37 publications

(4 citation statements)

References 33 publications

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“…It is thus possible that autocrine growth stimulation involving PDGF-BB is an important step in the formation of malignant mesothelioma (Versnel et al, 1991). In addition, large amounts of hyaluronan often accumulate in mesothelioma patients (Waxler et al, 1979;Arai et al, 1979). It is thus possible that PDGF, or other factors that are produced by mesothelioma cells, stimulate the production of hyaluronan by the surrounding mesothelial cells or fibroblasts, or by the mesothelioma cells themselves.…”

Section: Discussionmentioning

confidence: 99%

Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells

Heldin¹,

Asplund²,

Ytterberg³

et al. 1992

Biochemical Journal

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The molecular mechanism involved in the stimulation of hyaluronan synthetase in normal human mesothelial cells was investigated. Exposure of mesothelial cells to platelet-derived growth factor (PDGF)-BB stimulated hyaluronan synthetase activity, measured in isolated membrane preparations, as well as hyaluronan secretion into the medium. The effect on hyaluronan synthetase was maximal after 6 h of treatment. In contrast, the stimulatory effect of transforming growth factor-beta 1 reached a maximum after 24 h. The stimulatory effect of PDGF-BB was inhibited by cycloheximide. The phosphotyrosine phosphatase inhibitor vanadate was found to stimulate hyaluronan synthetase activity, and to potentiate the effect of PDGF-BB. The protein kinase C (PKC) stimulator phorbol 12-myristate 13-acetate (PMA) also stimulated hyaluronan synthetase; furthermore, depletion of PKC by preincubation of the cells with PMA led to an inhibition of the PDGF-BB-induced stimulation of hyaluronan synthetase activity. Thus the PDGF-BB-induced stimulation of hyaluronan synthetase activity is dependent on protein synthesis and involves tyrosine phosphorylation and activation of PKC.

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Section: Discussionmentioning

confidence: 99%

Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells

Heldin¹,

Asplund²,

Ytterberg³

et al. 1992

Biochemical Journal

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“…In fact, tumor-secreted HA in the pleural fluid has been suggested as a means of identifying MPMs [31, 33, 81, 86, 90–93]. In this context, Waxler and coworkers [94] developed a method for isolating HA and other GAGs from tumor tissues and observed that MPMs contained only or almost entirely HA, whereas carcinomas and sarcomas consisted of a mixture of HA and other GAGs. Based on this, these authors concluded HA as the sole or major GAG to confirm a diagnosis of MPM.…”

Section: Diagnostic Value Of Ha In Mpmmentioning

confidence: 99%

CD44 and its ligand hyaluronan as potential biomarkers in malignant pleural mesothelioma: evidence and perspectives

Cortes-Dericks

Schmid

2017

Respir Res

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Malignant pleural mesothelioma (MPM) is a rare and highly drug resistant tumor arising from the mesothelial surfaces of the lung pleura. The standard method to confirm MPM is the tedious, time-consuming cytological examination of cancer biopsy. Biomarkers that are detectable in pleural effusion or patient serum are reasonable options to provide a faster and noninvasive diagnostic approach. As yet, the current biomarkers for MPM lack specificity and sensitivity to discriminate this neoplasm from other lung tumors. CD44, a multifunctional surface receptor has been implicated in tumor progression in different cancers including MPM. The interaction of CD44 with its ligand, hyaluronan (HA) has demonstrated an important role in modulating cell proliferation and invasiveness in MPM. In particular, the high expression levels of these molecules have shown diagnostic relevance in MPM. This review will summarize the biology and diagnostic implication of CD44 and HA as well as the interaction of both molecules in MPM that will demonstrate their potential as biomarkers. Augmentation of the current markers in MPM may lead to an earlier diagnosis and management of this disease.

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“…An analysis of hyaluronan in these fluids has been tried as a diagnostic tool for mesothelioma, but it has not proven reliable in differentiating mesotheliomas from other malignant condition^.^^-^^"^-'^ I n order to increase the reliability of hyaluronan analyses, they have also been performed on tissue specimens from tumors, and it has been reported that high hyaluronan concentrations then show a better correlation with the diagnosis of mesothelioma. '0~'5~'7- 30 The development of new, specific assays for hyalurhas made it feasible to analyze the polysaccharide in serum. There so far has been only one short report of hyaluronan in the serum of a small number of cancer patients under treatment.32 As far as we know, no one has described hyaluronan in serum from patients with mesothelioma compared to patients with other malignant diseases.…”

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confidence: 99%

Concentration of hyaluronan in the serum of untreated cancer patients with special reference to patients with mesothelioma

Dahl

Laurent

1988

Cancer

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The concentration of hyaluronan was measured in the serum from patients with tumors. The patients were divided into nine groups: two control groups, i.e., those with benign tumors and those having undergone radical surgery; and seven groups of patients with untreated malignant conditions, i.e., mesotheliomas, sarcomas, lymphomas, breast carcinomas, brain tumors, bronchial carcinomas, and a group of various malignancies. As an additional control group, subjects with benign pulmonary diseases were investigated. The control groups and all the groups with malignant tumors except the mesotheliomas had serum hyaluronan values equal to or only slightly higher than those of healthy volunteers of the same age. The patients with mesotheliomas had significantly elevated hyaluronan levels (287 +/- 282 [Standard deviation] micrograms/l; n = 35; P less than 0.001) compared with healthy volunteers (54 +/- 28 micrograms/l in the age group of 51 to 60 years). Patients with asbestosis do not exhibit increased serum hyaluronan. The analysis of serum hyaluronan should therefore be of value in the diagnosis of mesothelioma.

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Electrophoresis of tissue glycosaminoglycans as an aid in the diagnosis of mesotheliomas

Cited by 37 publications

References 33 publications

Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells

Characterization of the molecular mechanism involved in the activation of hyaluronan synthetase by platelet-derived growth factor in human mesothelial cells

CD44 and its ligand hyaluronan as potential biomarkers in malignant pleural mesothelioma: evidence and perspectives

Concentration of hyaluronan in the serum of untreated cancer patients with special reference to patients with mesothelioma

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