Electrophoresis of Soluble Proteins Extracted from Cell Suspensions of Neonatal Rat Epidermis

Stern, Irving B.; Sekeri-Pataryas, Kalliope H.; Rabin, Myron

doi:10.1111/1523-1747.ep12676622

Cited by 4 publications

(1 citation statement)

References 19 publications

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“…Disc electrophoresis patterns have recently been published for neonatal rat epidermal cell fractions by Stern et al (9,3). Our results seem most similar to their keratinized fraction (as might be expected) and, taking differences in technique into account, their peak " I" (9), or the regions "e ,f ,g" (3), ma y be comparable to our peak 6.…”

Section: Discussionsupporting

confidence: 89%

Analysis of soluble proteins in comedones

Js¹,

Dt²,

Pe³

et al. 1977

Acta Derm Venereol

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The detergent-soluble fraction of purified, concentrated comedo samples was analysed for protein by disc-gel electrophoresis and fluorescamine assay. Spectrophotometer scanning revealed a characteristic pattern of 14 peaks which was essentially uniform in comedones from individuals having acne, Favre-Racouchot syndrome, or prepubertal comedones. The pattern was different in material derived from keratinous cysts and the contents of follicles from the nose. The presence of small amounts of serum proteins, notably globulins, was detected by immunodiffusion.

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Section: Discussionsupporting

confidence: 89%

Analysis of soluble proteins in comedones

Js¹,

Dt²,

Pe³

et al. 1977

Acta Derm Venereol

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The Identification Of Fibrous Proteins In Fetal Rat Epidermis By Electrophoretic And Immunologic Techniques

Dale¹,

Stern²,

Rabin³

et al. 1976

Journal of Investigative Dermatology

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Two proteins have been identified in extracts of fetal rat skin which are related to the two major fibrous proteins of newborn rat stratum corneum. The relative amount of these proteins increases daily from the 16th to the 20th day (d) of gestation when judged by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis and immunoelectrophoresis using antibody to the purified fibrous protein. Two-dimensional analysis by SDS-polyacrylamide gel electrophoresis and immunoelectrophoresis demonstrates that these two proteins are the only cross-reactive species in the fetal skin from 16d to 19d development. Some additional lower-molecular-weight components can be detected at 20d and 21d. In double-diffusion analysis, cross-reactive proteins in 19d fetal extracts show partial identity but have fewer antigenic sites than proteins in 20d extracts. The 20d protein shows a reaction of identity with purified newborn fibrous protein. Immunofluorescence studies on fetal skin support the prescence of cross-reacting components at 16d development related to the newborn fibrous protein. Intensity of fluorescence increases at 18d and 20d in the spinous and granular cell cytoplasm and in the keratohyaline granules. The stratum corneum, first seen at 20d, is intensely fluorescent. The cellular localization and time of appearance of the cross-reactive proteins suggest that they may be associated with tonofilaments.

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Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Of Proteins Of Newborn Rat Skin I. Cell Strata and Nuclear Proteins

Dale

Stern

1975

Journal of Investigative Dermatology

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The proteins obtained from separated cells of neonatal rat dermis, four cell populations of epidermis, and an epidermal nuclear preparation were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Comparison of the results of the insoluble proteins of the dermis and epidermis show no similarity of the major protein bands, indicating the effective separation of the dermis and epidermis and absence of cross-contamination. The gels of the soluble proteins of the basal, spinous, and granular layers of the epidermid are very similar. Only the pattern of bands of the cornified cells differs in that some of these bands are absent and at least three new bands are present. The insoluble proteins have specific differences in the protein content related to the cell structure. An example is the nuclear protein bands which correspond with the most prominent bands in the gels of basal and spinous layer proteins, but are absent, with the possible exception of one band, from gels of cornified cell proteins.

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Electrophoresis of Soluble Proteins Extracted from Cell Suspensions of Neonatal Rat Epidermis

Cited by 4 publications

References 19 publications

Analysis of soluble proteins in comedones

Analysis of soluble proteins in comedones

The Identification Of Fibrous Proteins In Fetal Rat Epidermis By Electrophoretic And Immunologic Techniques

Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis Of Proteins Of Newborn Rat Skin I. Cell Strata and Nuclear Proteins

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