Electrophoresis of Soft Colloids: Basic Principles and Applications

Duval, Jérôme F. L.

doi:10.1002/9780470024539.ch7

Cited by 13 publications

(26 citation statements)

References 73 publications

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“…fibrils) [43] may also provide structural information that is more easily related to environmental function. Similar arguments are valid for other colloidal characterisation techniques such as electrophoresis [34], which provides an estimate of the charge/size ratio of the colloids [34,44], although conversions of electrophoretic mobilities often require significant and complex interpretation [45].…”

Section: Non-size-based Measurements Of Colloids and Particlesmentioning

confidence: 64%

Environmental Colloids and Particles: Current Knowledge and Future Developments

Lead

Wilkinson

2006

Environmental Colloids and Particles

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Section: Non-size-based Measurements Of Colloids and Particlesmentioning

confidence: 64%

Environmental Colloids and Particles: Current Knowledge and Future Developments

Lead

Wilkinson

2006

Environmental Colloids and Particles

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“…3 However, the basic concept of a zeta-potential is not applicable to soft NPs due to the absence of a discrete slip plane at the interphase they form with the electrolytic medium (see ref 4 and references cited therein). The formalisms for electrokinetics of soft particles differ according to their treatment of electrostatics, in particular their ability to apply to thin double layer cases ( p 1 r   ), [5][6][7][8] or to the degree of sophistication in integrating particle backbone material distribution which impacts on both the electrostatic and hydrodynamic flow field distributions and, in turn, the particle electrophoretic mobility, µ. [7][8][9] The model by Ohshima 5 has been successfully employed to determine the electrostatic surface properties of various particles, including microgels, erythrocytes, and bacterial surfaces.…”

Section: Ohshima Formalism For the Electrophoretic Mobility Of Soft Cmentioning

confidence: 99%

“…[7][8][9] The model by Ohshima 5 has been successfully employed to determine the electrostatic surface properties of various particles, including microgels, erythrocytes, and bacterial surfaces. 4,6 Specifically, the electrophoretic mobility µ of soft particles that meet the conditions for establishment of a Donnan phase, i.e. p 1 r   , reads as: 1C in main text), asymptotically reaches a non-zero plateau value at sufficiently large electrolyte concentrations, which is a direct consequence of their defining ion permeability features.…”

Section: Ohshima Formalism For the Electrophoretic Mobility Of Soft Cmentioning

confidence: 99%

Rigorous Physicochemical Framework for Metal Ion Binding by Aqueous Nanoparticulate Humic Substances: Implications for Speciation Modeling by the NICA-Donnan and WHAM Codes

Town

Leeuwen

Duval

2019

Environ. Sci. Technol.

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Latest knowledge on the reactivity of charged nanoparticulate complexants toward aqueous metal ions is discussed in mechanistic detail. We present a rigorous generic description of electrostatic and chemical contributions to metal ion binding by nanoparticulate complexants, and their dependence on particle size, particle type (i.e., reactive sites distributed within the particle body or confined to the surface), ionic strength of the aqueous medium, and the nature of the metal ion. For the example case of soft environmental particles such as fulvic and humic acids, practical strategies are delineated for determining intraparticulate metal ion speciation, and for evaluating intrinsic chemical binding affinities and heterogeneity. The results are compared with those obtained by popular codes for equilibrium speciation modeling (namely NICA-Donnan and WHAM). Physicochemical analysis of the discrepancies generated by these codes reveals the a priori hypotheses adopted therein and the inappropriateness of some of their key parameters. The significance of the characteristic time scales governing the formation and dissociation rates of metal–nanoparticle complexes in defining the relaxation properties and the complete equilibration of the metal–nanoparticulate complex dispersion is described. The dynamic features of nanoparticulate complexes are also discussed in the context of predictions of the labilities and bioavailabilities of the metal species.

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“…The analysis disregards the flow permeation properties of the bacteriophage, which is a priori inconsistent with the subsurface viral structure that exhibits pores (41,44), the size of which exceeds tens of angstroms. For such soft particles, the very notion of z-potential has no physical meaning, since the gradual decay of the flow field within the particle makes the location of any slip plane irrelevant (45). Only for particles with moderate hydrodynamic permeability, one may use the z-potential concept for interpreting electrokinetic data, providing, however, that tangential surface conduction processes are taken into account in the electrokinetic model.…”

Section: Introductionmentioning

confidence: 99%

Impact of Chemical and Structural Anisotropy on the Electrophoretic Mobility of Spherical Soft Multilayer Particles: The Case of Bacteriophage MS2

Langlet

Gaboriaud

Gantzer

et al. 2008

Biophysical Journal

132

223

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We report a theoretical investigation of the electrohydrodynamic properties of spherical soft particles composed of permeable concentric layers that differ in thickness, soft material density, chemical composition, and flow penetration degree. Starting from a recent numerical scheme developed for the computation of the direct-current electrophoretic mobility (mu) of diffuse soft bioparticles, the dependence of mu on the electrolyte concentration and solution pH is evaluated taking the known three-layered structure of bacteriophage MS2 as a supporting model system (bulk RNA, RNA-protein bound layer, and coat protein). The electrokinetic results are discussed for various layer thicknesses, hydrodynamic flow penetration degrees, and chemical compositions, and are discussed on the basis of the equilibrium electrostatic potential and hydrodynamic flow field profiles that develop within and around the structured particle. This study allows for identifying the cases where the electrophoretic mobility is a function of the inner structural and chemical specificity of the particle and not only of its outer surface properties. Along these lines, we demonstrate the general inapplicability of the notions of zeta potential (zeta) and surface charge for quantitatively interpreting electrokinetic data collected for such systems. We further shed some light on the physical meaning of the isoelectric point. In particular, numerical and analytical simulations performed on structured soft layers in indifferent electrolytic solution demonstrate that the isoelectric point is a complex ionic strength-dependent signature of the flow permeation properties and of the chemical and structural details of the particle. Finally, the electrophoretic mobilities of the MS2 virus measured at various ionic strength levels and pH values are interpreted on the basis of the theoretical formalism aforementioned. It is shown that the electrokinetic features of MS2 are to a large extent determined not only by the external proteic capsid but also by the chemical composition and hydrodynamic flow permeation of/within the inner RNA-protein bound layer and bulk RNA part of the bacteriophage. The impact of virus aggregation, as revealed by decreasing diffusion coefficients for decreasing pH values, is also discussed.

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Electrophoresis of Soft Colloids: Basic Principles and Applications

Cited by 13 publications

References 73 publications

Environmental Colloids and Particles: Current Knowledge and Future Developments

Environmental Colloids and Particles: Current Knowledge and Future Developments

Rigorous Physicochemical Framework for Metal Ion Binding by Aqueous Nanoparticulate Humic Substances: Implications for Speciation Modeling by the NICA-Donnan and WHAM Codes

Impact of Chemical and Structural Anisotropy on the Electrophoretic Mobility of Spherical Soft Multilayer Particles: The Case of Bacteriophage MS2

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