Electrophoresis of Myocardial Cells

Quality testing of human albumin by capillary electrophoresis using thermally cross‐linked poly(vinyl pyrrolidone)‐coated fused‐silica capillary

“…CE is one of the most powerful liquid-phase separation techniques and is widely applied for the separation of biomolecules, such as amino acids [1,2], peptides [3,4], DNA [5,6], carbohydrates [7,8], and proteins [9][10][11]. In addition, it has been recognized that one prominent feature of CE is its low sample consumption; this advantage makes the technique economically efficient in monitoring the quality of expensive and short-supplied biopharmaceuticals, such as human albumin (HA).…”

“…However, there are some problems encountered in proteomics research that are caused by nonspecific protein adsorption to the fused-silica capillary inner surface, which lead to peak tailing, band broadening, and therefore poor separation efficiency, low recovery, and even the impossible detection of proteins. Over the last two decades, several strategies have been employed to reduce pro-tein adsorption (especially basic protein adsorption), including the use of extreme pH [12,13], high ionic strength [14], background electrolyte additives [15][16][17], covalently bonded coatings [18,19], noncovalently (physically adsorbed) bonded coatings [11,[20][21][22][23][24], and hybrid coatings [25][26][27]. Coating the fused-silica capillary inner surface with polymers has been the most common approach to date, and numerous polymers resistant to protein adsorption have been used to prepare coated capillaries [28][29][30][31][32][33][34].…”