Electrophoresis of a charge-regulated soft sphere: Importance of effective membrane charge

Tseng, Shiojenn; Hsieh, Tsung-Hsien; Yeh, Li‐Hsien; Wang, Nan; Hsu, Jyh‐Ping

doi:10.1016/j.colsurfb.2012.09.014

Cited by 6 publications

(2 citation statements)

References 65 publications

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“…In order to further understand the effect of digoxin on the surface potential of cardiomyocytes, we used a method of decomposing cell surface potential under the premise of ensuring cell survival. Due to the distance between the plasma membrane layer and the surface glycoconjugates is 50 nm, which is much larger than the usual thickness of the electric double layer 17,18 . Theoretically, when the concentration of extracellular K + is 140 mM, the theoretical value of the cell transmembrane potential is zero.…”

Section: Autorhythmicitymentioning

confidence: 92%

Relationship among surface electric double layer of cardiomyocyte membrane and toxicology of digoxin and opening of ion channels

Zhou

Hao

Sun

et al. 2022

Sci Rep

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We applied a new idea that the potential effect can change the ion adsorption structure on the cell surface to explore the mechanism of digoxin poisoning and the regulation of ion channels. The effects of digoxin on the electrophoretic mobility and behaviors (non-contraction or contraction or autorhythmicity) of cardiomyocytes were observed by single-cell electrophoresis technique (imitate the opening method of in vivo channel) and the method of decomposing surface potential components on the cells. As well as affect the association with electrical activity. The results suggested that the increase of cardiomyocytes transmembrane potential and the Na+–K+ exchange on the cell surface of the action potential phase 4 caused by the poisoning dose of digoxin, leading to the oscillation of adsorbed ions on the cell surface and the incomplete channel structure, which were the mechanism of cardiac ectopic beats. The results revealed that the opening of ion channels is regulated by the surface electric double layer of the cell membrane.

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Section: Autorhythmicitymentioning

confidence: 92%

Relationship among surface electric double layer of cardiomyocyte membrane and toxicology of digoxin and opening of ion channels

Zhou

Hao

Sun

et al. 2022

Sci Rep

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“…The current theory describing this effect is based on the chargeregulated nature of the two surfaces, which results in both charge and electrostatic potential variation as a function of separation distance [1][2][3][4][5][6][7][8][9][10]. When a surface with ionizable groups approaches another surface, electroneutrality requires the counterion concentration to increase in the solution between the surfaces to offset the decrease in inter-spatial volume.…”

Section: Introductionmentioning

confidence: 99%

Variation in bacterial ATP concentration during rapid changes in extracellular pH and implications for the activity of attached bacteria

Albert

Brown

2015

Colloids and Surfaces B: Biointerfaces

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In this study we investigated the relationship between a rapid change in extracellular pH and the alteration of bacterial ATP concentration. This relationship is a key component of a hypothesis indicating that bacterial bioenergetics - the creation of ATP from ADP via a proton gradient across the cytoplasmic membrane - can be altered by the physiochemical charge-regulation effect, which results in a pH shift at the bacteria's surface upon adhesion to another surface. The bacterial ATP concentration was measured during a rapid change in extracellular pH from a baseline pH of 7.2 to pH values between 3.5 and 10.5. Experiments were conducted with four neutrophilic bacterial strains, including the Gram-negative Escherichia coli and Pseudomonas putida and the Gram-positive Bacillus subtilis and Staphylococcus epidermidis. A change in bulk pH produced an immediate response in bacterial ATP, demonstrating a direct link between changes in extracellular pH and cellular bioenergetics. In general, the shifts in ATP were similar across the four bacterial strains, with results following an exponential relationship between the extracellular pH and cellular ATP concentration. One exception occurred with S. epidermidis, where there was no variation in cellular ATP at acidic pH values, and this finding is consistent with this species' ability to thrive under acidic conditions. These results provide insight into obtaining a desired bioenergetic response in bacteria through (i) the application of chemical treatments to vary the local pH and (ii) the selection and design of surfaces resulting in local pH modification of attached bacteria via the charge-regulation effect.

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Electrophoretic Behavior ofpH‐Regulated Soft Biocolloids

Yeh

Hsu

2016

Encyclopedia of Biocolloid and Biointerface Science 2V Set

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Electrophoresis of a charge-regulated soft sphere: Importance of effective membrane charge

Cited by 6 publications

References 65 publications

Relationship among surface electric double layer of cardiomyocyte membrane and toxicology of digoxin and opening of ion channels

Relationship among surface electric double layer of cardiomyocyte membrane and toxicology of digoxin and opening of ion channels

Variation in bacterial ATP concentration during rapid changes in extracellular pH and implications for the activity of attached bacteria

Electrophoretic Behavior ofpH‐Regulated Soft Biocolloids

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