Electrophoresis karyotype and chromosome-length polymorphism ofHistoplasma capsulatumclinical isolates from Latin America

Abstract

Intact chromosomes of 19 clinical isolates of Histoplasma capsulatum recently obtained in Argentina, Mexico and Guatemala and the laboratory reference strain G186B from Panama were analyzed using pulsed-field gel electrophoresis. Chromosomal banding patterns of the human isolates revealed 5-7 bands, ranging from 1.3 to 10 Mbp in size. Strain G186B showed five bands of approximately 1.1, 2.8, 3.3, 5.4 and 9.7 Mbp. Thirteen different electrokaryotypes were identified, indicating that the genome of H. capsulatum varies widely in nature, as observed previously in laboratory strains. No definite association was found between electrokaryotype and geographical or clinical source.

Chromosomal Polymorphism in the Sporothrix schenckii Complex

“…They found no correlation between karyotype profiles and clinical/epidemiological characteristics. Canteros et al [45] also found no correlation between the electrophoretic karyotype and geographic region in Histoplasma capsulatum isolated from Latin American patients. Thus, our results were consistent with those on other thermodimorphic fungi.…”

Genome Sequences Reveal Cryptic Speciation in the Human Pathogen Histoplasma capsulatum

“…duboisii H88 ( https://www.ncbi.nlm.nih.gov/bioproject/29163 ; also deposited on Dryad [ https://doi.org/10.5061/dryad.006bf ]), currently assembled into 16 supercontigs using bwa version 0.7.12 ( 79 ). Histoplasma strains vary in their karyotype and are thought to have between five and seven chromosomes, depending on the strain ( 80 , 81 ). The resulting bam files were merged using Samtools version 0.1.19.…”

Genetic diversity of Histoplasma capsulatum isolated from infected bats randomly captured in Mexico, Brazil, and Argentina, using the polymorphism of (GA)n microsatellite and its flanking regions

“…Histoplasma capsulatum have been classified by their DNA polymorphisms using molecular assays, which revealed genetic diversity depending on the source and geographic origin of the fungal isolates (Vincent et al 1986;Spitzer et al 1989Spitzer et al , 1990Keath et al 1992;Poonwan et al 1998;Salas-Rios et al 1998;Kasuga et al 1999;2003;Reyes-Montes et al 1999;Jiang et al 2000;Muniz et al 2001Muniz et al , 2010Canteros et al 2005;Komori et al 2005;Taylor et al 2005;Zancope-Oliveira et al 2005).…”