Electrophoresis andimmunoelectrophoresis of neonatal tears

Allerhand, Jona; Karelitz, Samuel; Penbharkkul, Saree; Ramos, Angela; Isenberg, Henry D.

doi:10.1016/s0022-3476(63)80076-1

Cited by 15 publications

(5 citation statements)

References 18 publications

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“…Serum albumin has been demonstrated in infants, 18 but this is the first report of identification in unstimulated tears. Serum albumin is not locally produced but derived from conjunctival blood vessels.…”

Section: Discussionmentioning

confidence: 74%

“…Lysozyme in infant tears was first identified in 1963. 18 Its concentration cannot diminish by stimulation, 14 which may be the reason why it attracted those researchers’ attention who used the invasive collection technique of Schirmer paper strips. A wide range of lysozyme concentrations has been reported in the literature (1.1, 1.6, 12 and 1.118 μg/μL 19 ).…”

Section: Discussionmentioning

confidence: 99%

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Tear Film Volume and Protein Analysis in Full-term Newborn Infants

Esmaeelpour¹,

Watts²,

Boulton³

et al. 2011

Cornea

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Purpose To investigate infant tear film secretion and protein profile, and to compare major tear proteins, such as immunoglobulin A, lactoferrin, and lysozyme, with those of adult controls. Methods Tears were collected, with a cellulose rod, from 40 healthy infants (19 female infants and 21 male infants, gestational duration: 39.71 ± 1.27 weeks) within 48 hours of birth and 22 adults (10 female infants and 12 male infants, mean age: 24.95 ± 3.63 years). A second collection was obtained from 14 of the infants (8 female infants and 6 male infants, postnatal age: 7.76 ± 6.14 weeks). The tear volume was measured, and protein in the samples was analyzed by Bradford assay and gel electrophoresis. Results Median tear volume (interquartile range) was 0.5μL (0.6– 2 μL) for newborn infants, 2.5μL (1.4–7.75 μL) for these infants at an older age, and 6μL (2.73–12.75 μL) in adults (P < 0.001, Kruskall–Wallis test). Immunoglobulin A concentration was significantly lower in newborns (P < 0.001, analysis of variance). Lipocalin was present in 36% of the newborn tear samples, whereas serum albumin was found in 86%. Mean protein concentration (μg/μL 6 SD) was 10.95 ± 5.51 in the newborns, 12.93 ± 3.99 in the older infants, and 13.04 ± 3.46 in the adults (P > 0.5, analysis of variance). Conclusions This is the first study reporting an investigation of unstimulated infant tears, using a noninvasive collection method. Tear protein content demonstrated that the infant tear film is different to that in adults.

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Section: Discussionmentioning

confidence: 74%

Section: Discussionmentioning

confidence: 99%

Tear Film Volume and Protein Analysis in Full-term Newborn Infants

Esmaeelpour¹,

Watts²,

Boulton³

et al. 2011

Cornea

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“…Fluorescein conjugation of antisera was performed as described by Peters and diffusioni. (1) Two moniospecific antisera mixed together: anti-secretory compollent and anti-a chain.…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulins, Secretory Component, and Transferrin in Eye Secretions of Infants in Regions With and Without Endemic Trachoma

1970

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Eye secretions were collected from Boston and Saudi Arab infants between 1 day and 6 months of age. These secretions were then tested with monospecific antisera in double diffusion and immunoelectrophoresis for the presence of immunoglobulin (Ig)A, IgM, IgG, secretory component, and transferrin. (The term "secretory component" has been chosen by the World Health Organization to replace the terms "secretory piece" and "transport piece.") Eye secretions of all newborns contained secretory component. IgA was found attached to the secretory component in samples from some infants as young as 8 days of age and in samples from most infants over 3 weeks of age. IgG and transferrin were found in most samples regardless of the age of the infant. The prevalence of the respective proteins in the eye secretions of Boston infants was found to be similar to their prevalence in the eye secretions of Saudi Arab infants, including four who developed trachoma during the study. Specific antibody to trachoma was demonstrated in the eye secretions of those infants with active trachoma, and the immunoglobulin class was determined.

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“…Total carbohydrate was measured with the anthrone reaction. The presence of proteins or polypeptides was demonstrated by the ability of electrophoretically separated moieties to react with Poinceau-S (Allerhand et al, 1963). The ethanol-ethyl ether layer of that particular mode of extraction was examined for the presence of lipid material by gas chromatography through the courtesy of Robert Rosenfeld, Sloan-Kettering Institute, New York, N.Y. Immunization.…”

Section: Methodsmentioning

confidence: 99%

IMMUNOLOGICAL AND TOXIC DIFFERENCES BETWEEN MOUSE-VIRULENT AND MOUSE-AVIRULENTCANDIDA ALBICANS

et al. 1963

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Immunological and toxic differences between mouse-virulent and mouse-avirulent Candida albicans. J. Bacteriol. 86:1010-1018. 1963.-The differences between mouse-avirulent and-virulent strains of Candida albicans reside at least in part in cell surface materials which can be extracted with solvents such as ethanolethyl ether and phenol. These extracts are complex haptens which behave like endotoxins in mice and rabbits. Antibodies produced against 1010

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Electrophoresis andimmunoelectrophoresis of neonatal tears

Cited by 15 publications

References 18 publications

Tear Film Volume and Protein Analysis in Full-term Newborn Infants

Tear Film Volume and Protein Analysis in Full-term Newborn Infants

Immunoglobulins, Secretory Component, and Transferrin in Eye Secretions of Infants in Regions With and Without Endemic Trachoma

IMMUNOLOGICAL AND TOXIC DIFFERENCES BETWEEN MOUSE-VIRULENT AND MOUSE-AVIRULENTCANDIDA ALBICANS

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