Electrophoresis and the Taxonomy of Saprophytic Fungi

Franke, Robert G.

doi:10.2307/2484505

Cited by 10 publications

(2 citation statements)

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“…It is known that isozyme variation in some fungi is influenced by growth substrate (Shannon et al 1973), by age of the fungus, and by the enzyme systems studied (Franke 1973). In our study, some of these effects were avoided or reduced by culturing all the isolates in the same growth medium and conditions, by comparing differing ages of the cultures, and by the random elements involved in selection of the enzymes.…”

Section: Phosphoglucose Isomerasementioning

confidence: 88%

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

Zhu¹,

Higginbotham²,

Dancik³

et al. 1988

Can. J. Bot.

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Mycelial extracts of 43 isolates of Suillus tomentosus (Kauffm.) Singer, Snell & Dick collected from four boreal forest regions in Alberta were subjected to starch gel electrophoresis. A total of 21 bands was resolved from eight different enzyme systems presumably representing 13 loci. Six loci were polymorphic among these isolates. Cluster and principal components analyses demonstrated that intraspecific genetic variability of this fungus existed among and within forest regions. Polymorphic loci of acid phosphatase and alkaline phosphatase exhibited the greatest genetic similarity among the isolates within forest regions. Habitat isolation and host selection could be the major sources of genetic variation among forest regions.

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Section: Phosphoglucose Isomerasementioning

confidence: 88%

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

Zhu¹,

Higginbotham²,

Dancik³

et al. 1988

Can. J. Bot.

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“…In fungi, the technique has mainly been used in taxonomic investigations where the emphasis is on differences between taxa (usually species) rather than on the variation within such groups (e.g. Franke, 1973;Sn^ider, 1973). Shipton & Fleischmann (1969) detected differences in total soluble protein profiles between species of Puccinia and, to a lesser extent, between formae speciales of the same species.…”

Section: Introductionmentioning

confidence: 99%

Variation for isozymes and double‐stranded RNA among isolates of Puccinia striiformis and two other cereal rusts

1985

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Gel electrophoresis was used to examine the variation in isozymes and dsRNA within and between the rust species Puccinia striiformis, P. recondita and P. hordei. No differences in isozyme phenotype were found among 29 diverse isolates of the wheat‐attacking form of P. striiformis (WYR). Smaller numbers of isolates of the barley‐attacking form (BYR) of P. recondita and of P. hordei showed similar intra‐group uniformity. There were major differences in isozyme phenotypes between the three species, while WYR and BYR differed for two of 12 enzymes. Double‐stranded RNA was detected in each species and in all 26 isolates examined. For WYR and BYR, all isolates within each group had the same or a similar phenotype. In contrast, each isolate of P. recondita and P. hordei had a unique phenotype. There were differences in dsRNA phenotypes both between the three species and between WYR and BYR. The uniformity of these rust populations and species for isozyme phenotype is contrasted with their variability in pathogenicity and with the variability in isozymes encountered in higher organisms. Uniformity may result from a feature of the biology of the rust species and populations examined, or from the relative homogeneity of the environment of biotrophic pathogens. Consistent differences in isozyme and dsRNA phenotype between the WYR and BYR isolates of P. striiformis indicate that these are two discrete populations, supporting the view that they should be recognized as f.sp. tritici and f.sp. hordei, respectively.

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From morphological to molecular: studies of myxomycetes since the publication of the Martin and Alexopoulos (1969) monograph

Stephenson

2011

Fungal Diversity

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Myxomycetes (plasmodial slime molds or myxogastrids) are a group of eukaryotic microorganisms usually present and sometimes abundant in terrestrial ecosystems. Because they produce aerial spore-bearing structures that resemble those of certain fungi and also typically occur in some of the same types of ecological situations as fungi, myxomycetes have been traditionally studied by mycologists, and this continued to be the case for the greater part of the twentieth century. However, there is now abundant molecular data to confirm that they are amoebozoans and not fungi. Efforts are currently underway to develop the first molecular phylogeny for myxomycetes, and several recent studies have used molecular techniques to detect the presence of these organisms in nature by means of direct environmental sampling.

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Electrophoresis and the Taxonomy of Saprophytic Fungi

Cited by 10 publications

References 0 publications

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

Intraspecific genetic variability of isozymes in the ectomycorrhizal fungus Suillus tomentosus

Variation for isozymes and double‐stranded RNA among isolates of Puccinia striiformis and two other cereal rusts

From morphological to molecular: studies of myxomycetes since the publication of the Martin and Alexopoulos (1969) monograph

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