Electrophilic thymol isobutyrate from Inula nervosa Wall. (Xiaoheiyao) ameliorates steatosis in HepG2 cells via Nrf2 activation

Cheng, Xiang‐Rong; Tu, Piao-Han; Dong, Wen-le; Yu, Bu-Tao; Xia, Shufang; Muskat, Mitchell N.; Guan, Bin

doi:10.1016/j.jff.2021.104895

Cited by 5 publications

(2 citation statements)

References 47 publications

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“…The membranes were further blocked with 5% non-fat milk, and then incubated with anti-Nrf2, anti-HO-1, anti-NQO1, or anti-β-actin at 4 °C overnight. Next, they were incubated with a secondary antibody for 1 h [ 30 ]. After washing with TBST 3 times, images were visualized via the Imaging System (Tanon, Shanghai, China) and analyzed by ImageJ (NIH, Bethesda, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

The Alleviation of Dextran Sulfate Sodium (DSS)-Induced Colitis Correlate with the logP Values of Food-Derived Electrophilic Compounds

Cheng

Song

et al. 2022

Antioxidants

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Food-derived electrophilic compounds (FECs) are small molecules with electrophilic groups with potential cytoprotective effects. This study investigated the differential effects of six prevalent FECs on colitis in dextran sodium sulfate (DSS)-induced mice and the underlying relationship with molecular characteristics. Fumaric acid (FMA), isoliquiritigenin (ISO), cinnamaldehyde (CA), ferulic acid (FA), sulforaphane (SFN), and chlorogenic acid (CGA) exhibited varying improvements in colitis on clinical signs, colonic histopathology, inflammatory and oxidative indicators, and Nrf2 pathway in a sequence of SFN, ISO > FA, CA > FMA, CGA. Representative molecular characteristics of the “penetration-affinity–covalent binding” procedure, logP value, Keap1 affinity energy, and electrophilic index of FECs were theoretically calculated, among which logP value revealed a strong correlation with colitis improvements, which was related to the expression of Nrf2 and its downstream proteins. Above all, SFN and ISO possessed high logP values and effectively improving DSS-induced colitis by activating the Keap1–Nrf2 pathway to alleviate oxidative stress and inflammatory responses.

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Section: Methodsmentioning

confidence: 99%

The Alleviation of Dextran Sulfate Sodium (DSS)-Induced Colitis Correlate with the logP Values of Food-Derived Electrophilic Compounds

Cheng

Song

et al. 2022

Antioxidants

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“…Previous studies have demonstrated that D. nervosa contains steroids, terpenoids, flavonoids, polysaccharides, and phenolic acids (4), which have a wide range of bioactivities including antiinflammatory, neuroprotective, antioxidant, hepatoprotective, and anticancer properties (5). Some studies have revealed that the root extracts protect HepG2 cells from H 2 O 2 -induced oxidative stress by increasing the expression of Nrf2 and related antioxidant enzymes (6).…”

Section: Introductionmentioning

confidence: 99%

Comparative bioactivity evaluation and metabolic profiling of different parts of Duhaldea nervosa based on GC-MS and LC-MS

Zhao,

Li,

et al. 2023

Front. Nutr.

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Duhaldea nervosa (Wallich ex Candolle) Anderberg has been widely used as medicine and food additive in China for a long history. Its roots, known as Xiaoheiyao, are the mainly used medicinal part, while the other tissues of D. nervosa are ignored as non-medicinal parts despite their high biomass, resulting in a huge waste of resources. To mine and expand the medicinal values of different parts of D. nervosa, metabolic analysis by GC/LC-MS and bioactivity evaluation were performed. Based on the antioxidant activity and correlation analysis, a metabolite-related network was constructed. A total of 45 volatile and 174 non-volatile compounds were identified. Among them, caffeoylquinic acids and derivatives were more abundant in roots and flowers, while coumaroyltartaric acids and derivatives were mainly present in stems and leaves. By multivariate analysis, 13 volatile and 37 non-volatile differential metabolites were found, respectively. In the bioactivity evaluation of different parts, the order of antioxidant capacity was flowers > roots > leaves or stems. The flowers showed the highest FRAP value (354.47 μM TE/g DW) and the lowest IC50 values in the DPPH (0.06 mg/mL) and ABTS (0.19 mg/mL) assay, while higher inhibitory activity against α-glucosidase was exhibited by flowers and leaves. This study first established the similarities and differences of phytochemicals and bioactivities in D. nervosa, providing a scientific basis for developing non-medicinal parts and guiding the clinical application of this medicinal and edible herb.

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Luteolin: Advances on Resources, Biosynthesis Pathway, Bioavailability, Bioactivity, and Pharmacology

Taban,

İlhan,

Süntar

2023

Handbook of Dietary Flavonoids

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Electrophilic thymol isobutyrate from Inula nervosa Wall. (Xiaoheiyao) ameliorates steatosis in HepG2 cells via Nrf2 activation

Cited by 5 publications

References 47 publications

The Alleviation of Dextran Sulfate Sodium (DSS)-Induced Colitis Correlate with the logP Values of Food-Derived Electrophilic Compounds

The Alleviation of Dextran Sulfate Sodium (DSS)-Induced Colitis Correlate with the logP Values of Food-Derived Electrophilic Compounds

Comparative bioactivity evaluation and metabolic profiling of different parts of Duhaldea nervosa based on GC-MS and LC-MS

Luteolin: Advances on Resources, Biosynthesis Pathway, Bioavailability, Bioactivity, and Pharmacology

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