Electrooxidation of pyrrole-terminated self-assembled lipoic acid derivatives

Cabrita, Joana F.; Viana, Ana S.; Eberle, Christoph; Montforts, Franz‐Peter; Mourato, Ana; Abrantes, L.M.

doi:10.1016/j.susc.2009.05.024

Cited by 11 publications

(7 citation statements)

References 34 publications

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“…After drying under pure nitrogen flow, they are flame-annealed under butane−oxygen to produce a flat surface with predominant (Au-111) crystallographic orientation. 37 For SPR observations, gold (50 nm) coated glass slides are purchased from Analytical μ-Systems and used as substrates. After washing with water and ethanol several times, they are placed in a UV cleaner for 25 min in order to remove any contamination.…”

Section: Methodsmentioning

confidence: 99%

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Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

et al. 2012

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Carbon disulfide (CS 2 ) can spontaneously react with amine groups to form dithiocarbamates on gold surface, providing the possibility to immobilize some compounds with primary or secondary amine groups in one step. Using this principle, an immunosensor interface prepared for immunoglobulin G (IgG) sensing surface toward anti-IgG has been fabricated for the first time by simply immersing gold slides into a mixed aqueous solution of CS 2 and protein A, followed by incubation in immunoglobulin G solution. The reaction between CS 2 and protein A has been followed by UV−vis spectroscopy, whereas cyclic voltammetry has been employed in the characterization of the modified gold surface with CS 2 and protein A, both methods indicating that protein A immobilization is implemented by CS 2 . Conventional ellipsometry, atomic force microscopy (AFM), as well as surface plasmon resonance (SPR) have been used to evaluate the specific binding of protein A with IgG and IgG with anti-IgG, revealing that IgG is specifically captured to form the biosensing interface, maintaining its bioactivity. Compared to direct adsorption of IgG on the gold surface, the IgG sensing surface constructed of CS 2 and protein A is far more sensitive to capture anti-IgG as its target molecule. In addition, the modified surface is proven to have good capability to inhibit nonspecific adsorption, as supported by control experiments using lysozyme and BSA. To conclude, antibody immobilization using this one-step method has potential as a simple and convenient surface modification approach for immunosensor development.

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Section: Methodsmentioning

confidence: 99%

“…The surfaces are cleaned in piranha solution (H 2 SO 4 /H 2 O 2 = 3:1, v/v) for a few minutes, and then washed with water and ethanol several times. After drying under pure nitrogen flow, they are flame-annealed under butane–oxygen to produce a flat surface with predominant (Au-111) crystallographic orientation …”

Section: Methodsmentioning

confidence: 99%

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

et al. 2012

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“…The substrates were cleaned in piranha solution (H 2 SO 4 :H 2 O 2 = 3:1, v/v) for few minutes, washed with water and ethanol, dried under N 2 (purity > 99.99997%) flow and flameannealed in a butane-oxygen flame [39] to produce a flat surface with predominant (1 1 1) crystallographic orientation, confirmed by STM. The average roughness factor of the substrates, 1.2, was estimated by the iodine chemisorption method [40] and is in agreement with reported values for similar thin gold electrodes [41,42].…”

Section: Substratesmentioning

confidence: 99%

“…As it is well known from the literature, SAMs bearing sulphur species as anchoring groups (e.g. thiols [11,12], disulphides [47] and thiotic acids [39]), desorb quantita- tively from gold surface in a strong alkaline solution, giving rise in general, to a sharp peak at negative potentials. The shape of this peak as well as the potential at which the desorption process occurs, reflecting monolayer stability, are strongly dependent on several factors, namely surface crystallinity, monolayer packing density and type of sulphur species [11,12].…”

Section: Covalent Attachment Of Electroactive Compounds Containing Ammentioning

confidence: 99%

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One step gold (bio)functionalisation based on CS2-amine reaction

Almeida

Cascalheira

Viana

2010

Electrochimica Acta

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Ultrathin polypyrrole films on self‐assembled monolayers as an efficient ultramicroelectrode assay

Bolat

Yaman

Kuralay

et al. 2020

J of Applied Polymer Sci

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In this work, we investigated the effect of electrodeposition of polypyrrole (PPy) films on two different self‐assembled monolayers (SAMs) modified gold electrode for the electrochemical construction of ultramicroelectrode (UME). In order to obtain SAM modified surfaces, 4‐mercapto‐1‐butanol and 11‐mercaptoundecanoic acid were used. The effect of these two chains on pyrrole electropolymerization was compared. Electropolymerization of pyrrole on SAM modified Au electrode was carried out by using cyclic voltammetry (CV) and constant potential electrolysis. To investigate the UME formation, the obtained surfaces were tested in Fe(CN)63−/4− redox system. UMEs were characterized using scanning electron microscopy energy‐dispersive X‐ray spectroscopy, attenuated total reflectance fourier transform infrared spectroscopy and electrochemical impedance spectroscopy. The designed UME was applied as an immobilization matrix to entrap a redox protein, Hemoglobin (Hb), as a model. Direct electron transfer between this protein and the fabricated thiol modified PPy based UME was achieved, which is very challenging on bare electrode surfaces. With this study, a simple, low‐cost and reproducible UME production way has been successfully accomplished and Hb modified UME is promising for different bioanalytical applications, for instance; cellular hydrogen peroxide or nitrite sensing.

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Electrooxidation of pyrrole-terminated self-assembled lipoic acid derivatives

Cited by 11 publications

References 34 publications

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

Antibody Oriented Immobilization on Gold using the Reaction between Carbon Disulfide and Amine Groups and Its Application in Immunosensing

One step gold (bio)functionalisation based on CS2-amine reaction

Ultrathin polypyrrole films on self‐assembled monolayers as an efficient ultramicroelectrode assay

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