Electroosmotic Thin Layer Chromatography with a Covered Sorption Layer

Berezkin, V. G.; Balushkin, A. O.; Tyaglov, B. V.; Rozhilo, Ya.; Малиновська, І. М.

doi:10.1023/b:dopc.0000048076.27488.8e

Cited by 3 publications

(8 citation statements)

References 2 publications

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“…Finally, the approach described here for generating quasistationary flow is potentially applicable to thin layer chromatography, particularly recent embodiments which require continuous flow. [8][9][10][11][12][26][27][28][29]…”

Section: Discussionmentioning

confidence: 99%

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

et al. 2009

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The sustained liquid flow of a typical lateral flow assay can be mimicked by two-dimensional shaped, thin porous membranes, specifically rectangular membranes appended to circular sectors. In designing these fan-shaped devices, we have been aided by analytical equations and finite-element simulations. We show both mathematically and experimentally how a continuous increase in unwetted pore volume causes a deviation from traditional imbibition, and leads to quasi-stationary flow in the rectangular element. These results are both theoretically and practically important because they indicate how medical diagnostic test strips may be fabricated without incorporating an absorbent pad.

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Section: Discussionmentioning

confidence: 99%

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

et al. 2009

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“…The reason for applying the pressure is to minimize mobile phase evaporation, resulting from Joule heating caused by the passage of electrical current through the mobile phase. [81,129,130] The applied potential that drives separations is usually supplied by a high voltage DC power supply. The power supply may be controlled by a computer, a programmable controller, a microprocessor, a timer, or the like, in order to precisely control the separation conditions for more reproducible results.…”

Section: Overview Of Instrument Requirementsmentioning

confidence: 99%

“…[130] Finally, Berezkin and coworkers present an alternative setup for PEC that does not require applied pressure, but simply a covered sorption layer and standard horizontal electrophoretic cell. [129] They argue that it is simply necessary to prevent mobile phase evaporation during PEC. Thus, according to their procedure, a thin polyethylene film is softened and applied to the absorption layer, resulting in strong adhesion between the film and the surface layer of absorbent particles on the plate.…”

Section: Peptides Proteins and Proteomics 1199mentioning

confidence: 99%

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Taking a Walk on the Wild Side with Planar Electrochromatography and Thin‐Layer Electrophoresis: Of Peptides, Proteins, and Proteomics

Patton

Panchagnula

Rockney

et al. 2006

Journal of Liquid Chromatography & Related Technologies

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Planar electrochromatography (PEC) and thin-layer electrophoresis (TLE) are examined for their potential application to peptide and protein analysis, employing one-dimensional (1D) and two-dimensional (2D) separations, which could potentially be useful for proteomics applications. The PEC and TLE literature are reviewed because the two methods are fundamentally similar in mechanical operations and performance. The application of TLE to peptide mapping is discussed and approaches to extending the technique to proteins and proteomics are offered. Much of the discussion is prognostic or hopefully predictive, attempting to ascertain how PEC might evolve in the coming years for peptide, protein, and ultimately proteomics applications.

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“…8 These effects can be partially eliminated by covering the layer but can be fully eliminated by pressurizing the layer, i.e., pressurized PEC (PPEC). 5,9,[12][13][14]4,7,9,10,[15][16][17][18] however, these devices have yet to be commercialized, perhaps due to their complexity. 19 Another challenge is the production of stationary phases designed for PPEC.…”

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confidence: 99%

Planar Electrochromatography Using an Electrospun Polymer Nanofiber Layer

Newsome

Olesik

2014

Anal. Chem.

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Electrospun polymer nanofiber stationary phases were examined for their application to planar electrochromatography (PEC). Separations were performed on polyacrylonitrile nanofiber ultra-thin-layer chromatography (UTLC) plates in 1-2 min using a ternary mobile phase. The influences of buffer concentration and pH, ratio of organic modifier, and development time on analyte migration distances were studied. Band broadening in this system was studied as a function of distance. The plate height initially decreased and then plateaued with a minimum plate height value as low as 11 μm. Nanofiber alignment considerably increased analyte migration rate, but larger spot sizes were noted when nearly complete fiber alignment was used. The easily tunable stationary phase thickness can be tailored to a given separation, where thinner layers promote faster separations and thicker layers are ideal for more complex mixtures. Compared to UTLC, PEC offers unique selectivity and decreased analysis time (>4 times faster over 15 mm). Results for a two-dimensional separation using UTLC and PEC are also reported. These rapid separations required 11 min using a 40 × 40 mm plate and exhibited a significant increase in separation number (70-77).

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Electroosmotic Thin Layer Chromatography with a Covered Sorption Layer

Cited by 3 publications

References 2 publications

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

Imbibition in Porous Membranes of Complex Shape: Quasi-stationary Flow in Thin Rectangular Segments

Taking a Walk on the Wild Side with Planar Electrochromatography and Thin‐Layer Electrophoresis: Of Peptides, Proteins, and Proteomics

Planar Electrochromatography Using an Electrospun Polymer Nanofiber Layer

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