Electronic study of fresh enzyme complexes of antifungal drugs-P450 and Aspergillus kojic acid biosynthesis. W: w saccharose flavus: fructose as a substratum

Abstract

Aspergillus(A) flavus cell contain the natural product cytochrome p450 enzyme, which is made up of from 400-500 amino acids and containing single heme group with single ferric ion, this enzyme destroyed by and reacted potentially with the used antifungal structures to yield new complexes with different electronic stability which related to the quality of the drug. The new complexes were studied by spectrophotometric technique. The theory on application it on the complexes indicated the following: low spin (LS) complexes with high crystal field stabilization energy (CFSE), octahedral shape and paramagnetic. Calculation and discussion pointed that the p450-triazole complex is the most stable and have higher stabilization energy. A flavus was grown on a carbon source formed from a combination of 1w:1w sucrose: fructose substrate to produce kojic acid by shake flask fermentation.The acid is detected and then identified by its physical properties as yellow crystalline material, odorless, melting point (m.p.) = 155-158(°C), lambda λmax=272nanometer (nm) in distilled water as pure solvent.