Electronic spectroscopic investigations of the stationary phase in reversed-phase liquid chromatography

Rutan, Sarah C.; Harris, Joel M.

doi:10.1016/0021-9673(93)80802-f

Cited by 74 publications

(65 citation statements)

References 47 publications

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“…16 Carr and co-workers found that typical C 18 stationary phases are more similar to 2-propanol than bulk liquid hexadecane. 17 On the basis of these studies, the stationary phase must be seen as a place where physical structure and chemical properties are intimately associated with solvent composition.…”

Section: Introductionmentioning

confidence: 98%

In Situ Examination of the Structure of Model Reversed-Phase Chromatographic Interfaces by Sum-Frequency Generation Spectroscopy

2003

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Model reversed-phase chromatographic interfacial systems were examined using sum-frequency generation (SFG) spectroscopy to study the effect of solvent on the structure and conformation of the stationary phase. Monolayers formed from mixed C 18 and C 1 alkysilanes (both polymeric octadecyltrichlorosilane-methyl trichlorosilane mixed monolayers and monomeric octadecyldimethylchlorosilane-trimethylchlorosilane mixed monolayers) on fused silica were examined in contact with air, deuterated acetonitrile (CD 3 CN), D 2 O, and mixtures of CD 3 CN and D 2 O. When mixed composition (C 18 and C 1 ) monolayers were examined, significant disorder was observed in the alkyl chains for all solvents examined at intermediate alkyl chain densities for the polymeric materials, and at all chain densities for the monomeric materials. Maximum order for intermediate polymeric chain densities was found to occur in contact with 40-50 vol % D 2 O solutions. These results indicate that solvent microheterogeneity has a large effect on alkyl chain order.

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Section: Introductionmentioning

confidence: 98%

In Situ Examination of the Structure of Model Reversed-Phase Chromatographic Interfaces by Sum-Frequency Generation Spectroscopy

2003

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“…Figure 6b showed the hierarchical tree diagram that characterized practically only column properties, i.e., it includes those parameters that are dependent on sorbent properties only (hydrophobicity, hydrophobic and methylene selectivities and steric selectivity) and partially micellar chromatographic property -hydrophilicity. In this figure, we also can clearly define two groups of columns that show similar properties: Hypersil and Nucleosil columns (one group) and Kromasil, Symmetry, LiChrospher, and Ultraspher columns (second group), but at the same time 8 closely related chromatographic properties. Similar to these columns properties Separon SGX C18 is showed.…”

Section: Cluster Analysismentioning

confidence: 81%

Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

Kulikov

Galat

2009

J of Separation Science

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Original PaperComparison of C18 silica bonded phases selectivity in micellar liquid chromatographyThe paper describes a new test designed in micellar LC (MLC) to compare the commercial C18 stationary phase properties. This test provides the total hydrophobicity, hydrophilicity, steric selectivity, hydrogen bonding, and ion-exchange capacity properties calculation of the ODS stationary phases. Both the test compounds and chromatographic separation conditions choice for column characterization in MLC are detailed. The chromatographic performance of several stationary phases that are used in MLC was evaluated with specific chromatographic test comprising nine test compounds, possessing different physico-chemical properties, which were injected on different supports with two micellar mobile phases: one at pH 7.0 (0.075 mol/L SDS and 1.5% v/v 1-pentanol), and other at pH 2.7 (0.075 mol/L SDS and 1.5% v/v 1-pentanol adjusted to pH by TFA). Fundamental column chromatographic properties were obtained under these conditions and were treated by hierarchical cluster analysis. From the results of cluster analysis, two closely related groups of columns are distinguished, and it was shown that the chosen column characteristic parameters allow characterizing both sorbent and micellar chromatographic system properties. Eleven columns were analyzed by this test, which allows a comparison of columns with the aim of the selection of suitable and analogous column for the analysis with MLC.

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“…Alternatively, it is a vehicle for the covalent or non-covalent binding of additional substrates. One of the most frequently utilized monolayer bonded interfaces in LC is the C 18 -on-silica interphase (Figure 1d) which has been the subject of intense scrutiny, having been characterized by NMR (44)(45)(46), Raman (47) and solvatochromic probe-based spectroscopies (48)(49)(50)(51)(52). Chapter 5 addresses the hydrolytic stability of conventional bonded silica interfaces that have a siloxane linkage ( Figure 6a).…”

Section: Bonded Monolayers (Chs 5 and 6)mentioning

confidence: 99%

The “Interface” in Analytical Chemistry: Overview and Historical Perspective

Helburn

2011

ACS Symposium Series

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Electronic spectroscopic investigations of the stationary phase in reversed-phase liquid chromatography

Cited by 74 publications

References 47 publications

In Situ Examination of the Structure of Model Reversed-Phase Chromatographic Interfaces by Sum-Frequency Generation Spectroscopy

In Situ Examination of the Structure of Model Reversed-Phase Chromatographic Interfaces by Sum-Frequency Generation Spectroscopy

Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography

The “Interface” in Analytical Chemistry: Overview and Historical Perspective

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