Electronic spectral detection in liquid chromatography

Biggs, Wilton R.; Fetzer, John C.

doi:10.1007/s00216-002-1346-3

Cited by 8 publications

(4 citation statements)

References 34 publications

(35 reference statements)

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“…DAD spectra are solvent-sensitive; comparing spectra from reversed phase runs in acetonitrile to reference spectra collected in methanol may not match due to a 1-2 nm shift for some compounds [11]. Spectra collected during solvent gradients may also suffer from this problem, and an even worse problem oc-curs due to different solvents in fluorescence detection [12]. The selectivity of FL detection is also very high, owing to the very low probability of coeluting compounds that coincide with the target analyte in excitation and emission wavelengths [12].…”

Section: Complementary Techniquesmentioning

confidence: 95%

“…Spectra collected during solvent gradients may also suffer from this problem, and an even worse problem oc-curs due to different solvents in fluorescence detection [12]. The selectivity of FL detection is also very high, owing to the very low probability of coeluting compounds that coincide with the target analyte in excitation and emission wavelengths [12]. Radiation with frequencies between 4000 and 400 cm -1 can be utilized in organic structure determination by making use of the fact that it is absorbed by bonds in organic compounds.…”

Section: Complementary Techniquesmentioning

confidence: 98%

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Analyzing transformation products of synthetic chemicals

Pérez

Petrović

Barceló

2008

The Handbook of Environmental Chemistry

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Section: Complementary Techniquesmentioning

confidence: 95%

Section: Complementary Techniquesmentioning

confidence: 98%

Analyzing transformation products of synthetic chemicals

Pérez

Petrović

Barceló

2008

The Handbook of Environmental Chemistry

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“…[17][18][19] The UV absorption detector, however, is an ideal detector with relatively high sensitivity, superior stability, wide detection range and good selectivity . [20,21] Combination of UV with GPC has been used for quantifying substances and for locating the location of certain groups in polymers . [22][23][24][25][26][27] In 2005, Lai et al [28 ] used GPC-UV to determine the degree of incorporation of surfactant in polystyrene latex particles, and developed a new method to quantitatively determine the degree of adhesion.…”

Section: Introductionmentioning

confidence: 99%

Accurate Quantitation of the Phenyl Group in Methylphenylsilicone Oils by GPC-UV

Kang

Zhang

et al. 2023

Chromatographia

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The phenyl content is an important quality parameter for phenyl silicone oils, yet its current determination method by 1 H NMR is still unsatisfactory due to the potential interference of methylphenylcyclosiloxane or solvents such as toluene and xylene. Herein, a novel method has been developed for the determination of the phenyl content in phenyl silicone oils by gel permeation chromatography-ultraviolet detection (GPC-UV) analysis. Under optimized parameters, the standard curve has been established in the linear range of 5-1000 µg/mL with a squared correlation coe cient of more than 0.999, and a quanti cation limit of 0.972 µg/mL. The GPC-UV method can easily exclude potential interfering substances, such as methylphenyl cyclosiloxane, toluene or xylene, and various silicone oils. The phenyl content in 9 available phenyl silicone oil samples was determined with a recovery in the range of 84.63%-106.74%. The determined phenyl content of sample H1 by GPC-UV was in good agreement with that (0.603 mg/g) by the 1 H-NMR analysis.

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“…[12] Furthermore, the damaging effects of B(e)p can be partially reversed by pre-treatment with memantine, resveratrol and genistein. [13] B(e)p with its structure of five fused aromatic rings (PAH) with a high molecular weight[14] can be found in substances besides cigarette smoke including automobile exhaust, charcoal-broiled foods, and incomplete combustion of organic materials. [15]…”

Section: Introductionmentioning

confidence: 99%

Effects of Benzo(e)pyrene on reactive oxygen/nitrogen species and inflammatory cytokines induction in human RPE cells and attenuation by mitochondrial-involved mechanism

Estrago-Franco¹,

Moustafa²,

Riazi‐Esfahani³

et al. 2016

J Ophthalmic Vis Res

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Purpose:To identify inhibitors that could effectively lower reactive oxygen/nitrogen species (ROS/RNS), complement and inflammatory cytokine levels induced by Benzo(e)pyrene [B(e)p], an element of cigarette smoke, in human retinal pigment epithelial cells (ARPE-19) in vitro.Methods:ARPE-19 cells were treated for 24 hours with 200 μM, 100 μM, and 50 μM B(e)p or DMSO (dimethyl sulfoxide)-equivalent concentrations. Some cultures were pre-treated with ROS/RNS inhibitors (NG nitro-L-arginine, inhibits nitric oxide synthase; Apocynin, inhibits NADPH oxidase; Rotenone, inhibits mitochondrial complex I; Antimycin A, inhibits mitochondria complex III) and ROS/RNS levels were measured with a fluorescent H2 DCFDA assay. Multiplex bead arrays were used to measure levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF), Transforming Growth Factor alpha (TGF-α) and Vascular Endothelial Growth Factor (VEGF). IL-6 levels were also measured by an enzyme-linked immunosorbent assay. Real-time qPCR analyses were performed with primers for C3 (component 3), CFH (inhibits complement activation), CD59 (inhibitor of the complement membrane attack complex (MAC)) and CD55/DAF (accelerates decay of target complement target proteins).Results:The ARPE-19 cultures treated with B(e)p showed significantly increased ROS/RNS levels (P < 0.001), which were then partially reversed by 6 μM Antimycin A (19%, P = 0.03), but not affected by the other ROS/RNS inhibitors. The B(e)p treated cultures demonstrated increased levels of IL-6 (33%; P = 0.016) and GM-CSF (29%; P = 0.0001) compared to DMSO-equivalent controls, while the expression levels for components of the complement pathway (C3, CFH, CD59 and CD55/DAF) were not changed.Conclusion:The cytotoxic effects of B(e)p include elevated ROS/RNS levels along with pro-inflammatory IL-6 and GM-CSF proteins. Blocking the Qi site of cytochrome c reductase (complex III) with Antimycin A led to partial reduction in B(e)p induced ROS production. Our findings suggest that inhibitors for multiple pathways would be necessary to protect the retinal cells from B(e)p induced toxicity.

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Electronic spectral detection in liquid chromatography

Cited by 8 publications

References 34 publications

Analyzing transformation products of synthetic chemicals

Analyzing transformation products of synthetic chemicals

Accurate Quantitation of the Phenyl Group in Methylphenylsilicone Oils by GPC-UV

Effects of Benzo(e)pyrene on reactive oxygen/nitrogen species and inflammatory cytokines induction in human RPE cells and attenuation by mitochondrial-involved mechanism

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