Electronic recording of lifetime locomotory activity patterns of adult medflies

Rodovitis, Vasilis G.; Papanastasiou, Stella A.; Bataka, Evmorfia P.; Nakas, Christos T.; Koulousis, Nikos A.; Carey, James R.; Papadopoulos, Nikos T.

doi:10.1371/journal.pone.0269940

Cited by 5 publications

(8 citation statements)

References 63 publications

(108 reference statements)

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“…In this system, flies were individually kept in 32 glass tubes with 25 mm diameter and 125 mm length. A vinyl plastic stopper (CAP25-BLK- Vinyl Tube Cap-Trikinetics) was adjusted inside on the one end of the tube, which was an agar-based gel diet with sugar, yeast hydrolysate, agar, nipagin and water (4:1:0.2:0.1:20), for food and water provision [ 28 ]. The other end of the tube was covered with a piece of organdie to allow ventilation.…”

Section: Methodsmentioning

confidence: 99%

Artificial diet alters activity and rest patterns in the olive fruit fly

et al. 2023

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Olive fruit flies, Bactrocera oleae (Diptera: Tephritidae) reared in the laboratory on an artificial diet are essential for the genetic control techniques against this pest. However, the colony’s laboratory adaptation can affect the quality of the reared flies. We used the Locomotor Activity Monitor to track the activity and rest patterns of adult olive fruit flies reared as immatures in olives (F2-F3 generation) and in artificial diet (>300 generations). Counts of beam breaks caused by the adult fly activity were used as an estimation of its locomotor activity levels during the light and dark period. Bouts of inactivity with duration longer than five minutes were considered a rest episode. Locomotor activity and rest parameters were found to be dependent on sex, mating status and rearing history. In virgin flies reared on olives, males were more active than females and increased their locomotor activity towards the end of the light period. Mating decreased the locomotor activity levels of males, but not of female olive-reared flies. Laboratory flies reared on artificial diet had lower locomotor activity levels during the light period and more rest episodes of shorter duration during the dark period compared to flies reared on olives. We describe the diurnal locomotor activity patterns of B. oleae adults reared on olive fruit and on artificial diet. We discuss how locomotor activity and rest pattern differences may affect the laboratory flies’ ability to compete with wild males in the field.

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Section: Methodsmentioning

confidence: 99%

Artificial diet alters activity and rest patterns in the olive fruit fly

et al. 2023

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“…In this system flies were individually kept in each of 32 glass tubes with 25 mm diameter and 125 mm length. On the one end of the tube, we adjusted a vinyl plastic stopper (CAP25-BLK-Vinyl Tube Cap-Trikinetics) inside which was an agar-based gel diet with sugar, yeast hydrolysate, agar, nipagin and water (4:1:0.2:0.1:20) for food and water provision [31]. The other end of the tube was covered with a piece of organdie to allow ventilation.…”

Section: Methodsmentioning

confidence: 99%

Laboratory rearing alters activity and sleep patterns in the olive fruit fly

Terzidou

Koveos

Papadopoulos

et al. 2022

Preprint

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Olive fruit flies, Bactrocera oleae (Diptera: Tephritidae) that are laboratory reared in artificial diet are essential for the genetic control techniques for this pest. However, the colony’s laboratory adaptation can affect their quality. We used the Locomotor Activity Monitor (LAM25, Trikinetics, MA, USA) to track the activity and sleep patterns of adult olive fruit flies reared as immatures in olives (F2-F3 generation) and in artificial diet (>300 generations). Counts of beam breaks caused by the adult fly activity were used as an estimation of its locomotor activity levels during the light and dark period. A longer than five minutes period of adults’ inactivity during the dark period was considered as one sleep episode. Activity levels and sleep parameters were found to be dependent on sex, mating status and rearing history. In virgin flies reared in olives, males were more active than females and increased their locomotor activity towards the end of the light period. Mating decreased the locomotor activity levels of males, but not of females olive-reared flies. Laboratory flies reared in artificial diet had lower locomotor activity levels during the light period and more sleep episodes of shorter duration compared to flies reared in olives. We describe the diurnal locomotor activity patterns of B. oleae adults reared in olive fruits and in artificial diet. We discuss how locomotor activity and sleep pattern differences may affect the laboratory flies’ ability to compete with wild males in the field.

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“…The protocol of Rodovitis et al (2022) was followed for both measuring detailed locomotor activity and maintaining the flies in the glass tubes. The day following adult emergence, we placed virgin females of the Mediterranean fruit fly laboratory strain "Benakio," which was maintained under laboratory conditions for more than 30 years (Kyritsis et al (2019)), in the tubes of the locomotory activity Monitor-LAM25 system and maintained them until death.…”

Section: Data D E S Cr I P Ti O Nmentioning

confidence: 99%

Daily activity profiles over the lifespan of female medflies as biomarkers of aging and longevity

Chen,

Müller,

Rodovitis

et al. 2024

Aging Cell

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The relationship between the early‐age activity of Mediterranean fruit flies (medflies) or other fruit flies and their lifespan has not been much studied, in contrast to the connections between lifespan and diet, sexual signaling, and reproduction. The objective of this study is to assess intra‐day and day‐to‐day activity profiles of female Mediterranean fruit flies and their role as biomarker of longevity as well as to explore the relationships between these activity profiles, diet, and age‐at‐death throughout the lifespan. We use advanced statistical methods from functional data analysis (FDA). Three distinct patterns of activity variations in early‐age activity profiles can be distinguished. A low‐caloric diet is associated with a delayed activity peak, while a high‐caloric diet is linked with an earlier activity peak. We find that age‐at‐death of individual medflies is connected to their activity profiles in early life. An increased risk of mortality is associated with increased activity in early age, as well as with a higher contrast between daytime and nighttime activity. Conversely, medflies are more likely to have a longer lifespan when they are fed a medium‐caloric diet and when their daily activity is more evenly distributed across the early‐age span and between daytime and nighttime. The before‐death activity profile of medflies displays two characteristic before‐death patterns, where one pattern is characterized by slowly declining daily activity and the other by a sudden decline in activity that is followed by death.

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Electronic recording of lifetime locomotory activity patterns of adult medflies

Cited by 5 publications

References 63 publications

Artificial diet alters activity and rest patterns in the olive fruit fly

Artificial diet alters activity and rest patterns in the olive fruit fly

Laboratory rearing alters activity and sleep patterns in the olive fruit fly

Daily activity profiles over the lifespan of female medflies as biomarkers of aging and longevity

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