Electronic properties and surface potential evaluations at the protein nano-biofilm/oxide interface: Impact on corrosion and biodegradation

Rahimi, Ehsan; Offoiach, Ruben; Lekka, Maria; Fedrizzi, L.

doi:10.1016/j.colsurfb.2022.112346

Cited by 5 publications

(21 citation statements)

References 75 publications

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“…Electrochemical measurements in Figure and XPS results show that the high adsorption of the protein on Mg in the NaCl solution is accompanied by improvement in the corrosion resistance (lower corrosion current density). This event can be explained by the formation of a thick or multilayer of the BSA protein (a strong metal–protein complex), with lower surface potential or electronic conductivity than the substrate, that strongly controls the whole charge transfer for the electrochemical interaction at the solid/protein/electrolyte interfaces (as fully described in Section and is discussed in the next section). , In PBS and Hanks’ environments containing the BSA protein, the corrosion resistance of the Mg alloy slightly decreased owing to the decline in the P and Ca/P intensity signals and particularly enhancing metal–protein complex formation. The self-protecting action of phosphate and calcium phosphate species against the corrosion and biodegradation processes diminished in BSA protein media due to the imperfect, thin protective film and nonhomogeneous distribution of phosphate and calcium phosphate products …”

Section: Resultsmentioning

confidence: 99%

“…They are considered to be electrically conductive soft materials, depending on their molecular structure . The specific electrical conductivity (EC) of adsorbed protein nanobiofilms on biomaterial surfaces can remarkably influence other biological events, particularly electrochemical interactions and metal ion release at the protein/oxide interface. , An extensive range of experimental approaches has been utilized to analyze the conductivity of biological species with a nanometer-scale structure, such as DNA and proteins. These techniques demonstrate valuable physical and chemical information regarding the EC of adsorbed DNA or protein molecules under both ex situ (in vacuum or air) and in situ (electrolyte media) conditions.…”

Section: Introductionmentioning

confidence: 99%

“…The high spatial resolution and substantial surface sensitivity of SKPFM to any chemical and physical alterations provide essential information about the protein nanobiofilm/metal or oxide interface, including adsorption morphology, electrical surface potential, and predictive insights into electrochemical interactions. , This specific information supported by the SKPFM approach is vital in the case of Mg and Mg alloy bioactive surfaces because they have a high rate of degradation and form complex corrosion products. Furthermore, particularly at the early stages of immersion in human body media, the morphology of the adsorbed protein and its electrical surface potential distribution can directly influence cell adhesion/deformation, the osseointegration process, and the long-term durability of implanted biomaterials…”

Section: Introductionmentioning

confidence: 99%

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Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

et al. 2022

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The formation of a protein nanobiofilm on the surface of degradable biomaterials such as magnesium (Mg) and its alloys influences metal ion release, cell adhesion/spreading, and biocompatibility. During the early stage of human body implantation, competition and interaction between inorganic species and protein molecules result in a complex film containing Mg oxide and a protein layer. This film affects the electrochemical properties of the metal surface, the protein conformational arrangement, and the electronic properties of the protein/Mg oxide interface. In this study, we discuss the impact of various simulated body fluids, including sodium chloride (NaCl), phosphate-buffered saline (PBS), and Hanks’ solutions on protein adsorption, electrochemical interactions, and electrical surface potential (ESP) distribution at the adsorbed protein/Mg oxide interface. After 10 min of immersion in NaCl, atomic force microscopy (AFM) and scanning Kelvin probe force microscopy (SKPFM) showed a higher surface roughness related to enhanced degradation and lower ESP distribution on a Mg-based alloy than those in other solutions. Furthermore, adding bovine serum albumin (BSA) to all solutions caused a decline in the total surface roughness and ESP magnitude on the Mg alloy surface, particularly in the NaCl electrolyte. Using SKPFM surface analysis, we detected a protein nanobiofilm (∼10–20 nm) with an aggregated and/or fibrillary morphology only on the Mg surface exposed in Hanks’ and PBS solutions; these surfaces had a lower ESP value than the oxide layer.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

et al. 2022

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“…This phenomenon can be explained by the formation of a thick or multilayer of the BSA protein, also known as a strong metal–protein complex . This layer has a lower electronic conductivity (less surface potential and/or surface charge) than the substrate, and it strongly controls the whole charge transfer for the electrochemical interaction that takes place at the interfaces . The presence of BSA in Hanks caused a modest decrease in the corrosion resistance of WE43 by diminishing the Ca/P and P intensity signals and, in particular, fostering metal-protein complex formation.…”

Section: Resultsmentioning

confidence: 99%

“…This sets it apart from other surface techniques that have limited applicability . The total WFE, the multipoles of the surface components, and the static charges are all strongly related to the surface potential or electrostatic interactions in any system of semiconductor or dielectric materials . In WE43, for instance, the electrical surface potential signal on the oxide layer is determined by the combined WFE of the oxide components, which is in turn determined by their weighted concentrations .…”

Section: Resultsmentioning

confidence: 99%

Albumin Protein Impact on Early-Stage In Vitro Biodegradation of Magnesium Alloy (WE43)

Imani,

Rahimi,

Lekka

et al. 2023

ACS Appl. Mater. Interfaces

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Mg and its alloys are promising biodegradable materials for orthopedic implants and cardiovascular stents. The first interactions of protein molecules with Mg alloy surfaces have a substantial impact on their biocompatibility and biodegradation. We investigate the early-stage electrochemical, chemical, morphological, and electrical surface potential changes of alloy WE43 in either 154 mM NaCl or Hanks' simulated physiological solutions in the absence or presence of bovine serum albumin (BSA) protein. WE43 had the lowest electrochemical current noise (ECN) fluctuations, the highest noise resistance (Z n = 1774 Ω•cm 2 ), and the highest total impedance (|Z| = 332 Ω•cm 2 ) when immersed for 30 min in Hanks' solution. The highest ECN, lowest Z n (1430 Ω•cm 2 ), and |Z| (49 Ω•cm 2 ) were observed in the NaCl solution. In the solutions containing BSA, a unique dual-mode biodegradation was observed. Adding BSA to a NaCl solution increased |Z| from 49 to 97 Ω•cm 2 and decreased the ECN signal of the alloy, i.e., the BSA inhibited corrosion. On the other hand, the presence of BSA in Hanks' solution increased the rate of biodegradation by decreasing both Z n and |Z| while increasing ECN. Finally, using scanning Kelvin probe force microscopy (SKPFM), we observed an adsorbed nanolayer of BSA with aggregated and fibrillar morphology only in Hanks' solution, where the electrical surface potential was 52 mV lower than that of the Mg oxide layer.

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Biodegradation of Oxide Nanoparticles in Apoferritin Protein Media: A Systematic Electrochemical Approach

Rahimi,

Kim,

Offoiach

et al. 2023

Adv Materials Inter

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Functional oxide nanoparticles are extensively utilized in the last decades for biomedical purposes due to their unique functional properties. Nevertheless, their biodegradation mechanism by biological species, particularly by proteins at oxide/protein interfaces, still remains limited. Here, a systematic approaches is provided to investigate electrochemical behavior, electronic properties, and biodegradation mechanism of cobalt ferrite (CFO) and cobalt ferrite‐bismuth ferrite (CFO‐BFO) core‐shell nanoparticles in apoferritin‐containing media. Scanning Kelvin probe force microscopy results indicate that the presence of a thin shell (≈5 nm) of BFO on CFO causes a significant increase in surface potential. The potentiodynamic polarization measurements in different solutions showed higher anodic current densities for both samples when decreasing pH and increasing apoferritin concentration. Notably, CFO‐BFO exhibits lower anodic current densities than CFO due to a slightly higher flat band potential and lower donor density distribution on CFO‐BFO than on CFO, which results in lower electrochemical activity. Long‐term monitoring reveals that biodegradation of both nanoparticles is accelerated by high apoferritin concentrations and acidic media, resulting in the decrease of electrochemical potentials and impedance values, and enhancement of metal ion release. Thus, this systematic biodegradation study can help to predict the lifespan and toxicity of these functional nanoparticles in biological environments.

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Electronic properties and surface potential evaluations at the protein nano-biofilm/oxide interface: Impact on corrosion and biodegradation

Cited by 5 publications

References 75 publications

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

Morphological and Surface Potential Characterization of Protein Nanobiofilm Formation on Magnesium Alloy Oxide: Their Role in Biodegradation

Albumin Protein Impact on Early-Stage In Vitro Biodegradation of Magnesium Alloy (WE43)

Biodegradation of Oxide Nanoparticles in Apoferritin Protein Media: A Systematic Electrochemical Approach

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