Electronic profiling of algae-derived DNA using DNA-specific Schottky diode

Azmi, Siti Zulfikriyah; Vello, Vejeysri; Rizan, Nastaran; Krishnasamy, Jegenathan; Talebi, Sara; Gunaselvam, Priya; Iqbal, Suhaina Nashath Mohamed; Yew, Chan Yen; Phang, Siew‐Moi; Iwamoto, Mitsumasa; Periasamy, Vengadesh

doi:10.1007/s00339-018-1972-z

Cited by 11 publications

(11 citation statements)

References 27 publications

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“…From the I-V graphs, semiconducting (rectifying) behavior can be observed where an insulating region at lower voltage is followed by a rapid increase of current beyond a threshold or turn-on voltage, [31][32][33][34] referring to the existence of a band gap between the valence and conduction bands. 8,36,37 As can be seen from the I-V profiles, each DNA sequence shows a specific turn-on voltage (poly A; 0.9, poly T; 1.2, poly C; 1.1, poly G; 0.85 V).…”

Section: Resultsmentioning

confidence: 99%

“…29,30 However, previous experimental and theoretical approaches involved difficult and complicated methodologies in addition to requiring sophisticated instruments. In this work, we propose an electronically derived technique using a DNA-specific Schottky junction [31][32][33][34] to quantitatively characterize and differentiate the electronic behaviour of DNA bases in the form of short ss poly A, poly T, poly C and poly G.…”

Section: Introductionmentioning

confidence: 99%

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Electronic Properties of Short Polynucleotides Studied Using Schottky Junctions

Daraghma

Talebi

Periasamy

2021

Journal of Elec Materi

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Deoxyribonucleic acid (DNA), the blueprint of life, has attracted recent attention concerning its potential applications in electronics. In order to realize these applications, charge transfer through the molecule has been subjected to numerous experimental and theoretical studies in the last few decades. As a result of varying experimental conditions, different electrical behaviors have been observed. The sensitive structure of DNA is influenced by extreme environmental conditions as shown in common characterization techniques. Finding a simple yet quantitative accurate method is more efficient for understanding the electronic properties of DNA. In this work, we have employed DNA-specific Schottky junctions integrated within a printed circuit board (PCB) to investigate the properties of the four nitrogenous bases of guanine (G), thymine (T), cytosine (C) and adenine (A) in short polynucleotide form. Acquisition and analysis of the current-voltage (I-V) profiles allowed measurement of selected solid-state parameters corresponding to each of the DNA polynucleotide base. While observing characteristic I-V profiles and parameters, significantly closer and higher conductive profiles were demonstrated for the purines (A and G) as compared to the highly similar profiles of the pyrimidines (T and C) which is in agreement with previous observations. The observations obtained from this work may, therefore, provide a clear conceptualization of the role of each nitrogenous base in charge transfer process through the DNA molecule and allow better understanding of the fingerprinting electronic properties of each base.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electronic Properties of Short Polynucleotides Studied Using Schottky Junctions

Daraghma

Talebi

Periasamy

2021

Journal of Elec Materi

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“…The glass slides were soaked in dishwashing soap and immersed in an ultrasonic bath (Thermo-6D Thermo-Line, rinse in deionised water [13]. The glass slides were blow-dried with nitrogen gas gun.…”

Section: Raman Spectroscopymentioning

confidence: 99%

Simplified Bioanalytical Approaches for the Characterization of Microalgae and Cyanobacteria

Iqbal¹,

Din²,

Bhassu³

2022

Act Scie Medic

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Harmful microalgae and cyanobacterial bloom cause deteriorated water quality and raises severe health concerns. This study discussed the demonstrations of simplified bioanalytical tools to enhance the natural resources of microalgae and cyanobacteria, to be given its importance, and how this knowledge can be used for wealth creation. Three bioanalytical approaches; (1) gas chromatography-mass spectrometry (GC-MS), (2) Raman spectroscopy, and (3) universal plastid biomarker, were successfully incorporated in selected microalgae and cyanobacteria. GC-MS analysis via solvent extraction characterized the organic compounds such as hydrocarbons and silicones derivatives. Air-dried thin layer of microalgae and cyanobacteria analyzed in Raman spectroscopy corresponded to three major spectra peaks, which fingerprints saffron and carotene photosynthetic pigments. Lastly, the taxonomic classification was simplified via the plastid 23S ribosomal ribonucleic acid (rRNA) biomarker, whereby, the Neighbor-Joining tree showed a clear separation between the phylogenetic clade of brown and green microalgae (eukaryotic), and cyanobacteria (prokaryotic). In short, this simplified multi-approaches; using molecular biomarkers provided an accurate identification on the species of microalgae and cyanobacteria, chemical biomarkers GC-MS characterized the organic compounds for sustainable green technology in industrial application, and finally, Raman spectroscopy can be future developed as a miniaturized handheld device for field sample detection for its fingerprinting characterization.

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“…The increasing number of research works on this new solid‐state material has collectively given rise to a new and exciting field of research aptly termed DNA electronics . DNA electronics is especially exciting as it opens up the possibility of developing rapid, sensitive, and effective genomic sequencers . The challenge, however, is related to the sensitivity of DNA molecules towards its various cellular environmental surroundings such as pH, temperature, ionic strength variations, pressure, salt, etc.…”

Section: Introductionmentioning

confidence: 99%

“…The challenge, however, is related to the sensitivity of DNA molecules towards its various cellular environmental surroundings such as pH, temperature, ionic strength variations, pressure, salt, etc. . Varying pH conditions, for example, have been found to impart significantly higher biochemical fluctuations in terms of DNA functionality in cellular environments …”

Section: Introductionmentioning

confidence: 99%

Effect of pH on the Conductivity of Basidiomycetes DNAs Integrated Within Schottky‐Like Junctions

et al. 2020

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Deoxyribonucleic acid (DNA) has been studied extensively for its unique self‐assembling behavior for applications in the field of molecular electronics. The study of sequence‐specific electronic properties of DNA is one such study which could potentially be utilized as a platform to understand its charge transfer mechanism useful for integration into DNA‐based electronics. However, DNA charge transfer are significantly regulated by various environmental factors, such as pH fluctuations which influences the stability of the double helix structure and strongly attenuates its’ electronic profile. Extreme pH conditions lead to aggravated structural and chemical changes leading to DNA damage, which could have profound biological consequences. In this work, pH influence on basidiomycetes DNAs integrated within an aluminium (Al)‐indium tin oxide (ITO) Schottky junction was investigated. In general, the junction structure exhibited Ohmic behaviour at high acidic (pH 1 to 2) and basic (pH 13 to 14) environments, while behaving as a semiconductive Schottky‐like junction at intermediate pH values. Furthermore, various solid‐state parameters calculated for the DNA enable the gathering of information towards understanding the charge transfer mechanism in DNA molecules as a function of pH conditions, which could act as a potential indicator for various stages of DNA functionality.

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Electronic profiling of algae-derived DNA using DNA-specific Schottky diode

Cited by 11 publications

References 27 publications

Electronic Properties of Short Polynucleotides Studied Using Schottky Junctions

Electronic Properties of Short Polynucleotides Studied Using Schottky Junctions

Simplified Bioanalytical Approaches for the Characterization of Microalgae and Cyanobacteria

Effect of pH on the Conductivity of Basidiomycetes DNAs Integrated Within Schottky‐Like Junctions

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