“…Fluorescence anisotropy is a technique that can provide useful information about the rotational motion of a fluorophore through measuring the polarization of the emitted fluorescence. The technique is a proven method for studying molecular mobility and have enabled the use of fluorescence anisotropy in the study of binding interactions, microviscosities of micellar systems, biological membranes, nucleic acids, proteins, and living cells. , Steady-state fluorescence anisotropy measurements are carried out by exciting the sample with vertically plane-polarized light. The parallel and perpendicular components of the fluorescence emission are measured using polarizers oriented with vertical and horizontal orientation, respectively.…”