“…Identical trends are observed in both cases even though MS detection was used for CE experiments alluding to the fact that the anisotropy experiment could have predicted separation conditions for optimal selectivity as manifested by the chiral resolution in the CE−MS experiment. 10 CE−MS data . (A) anisotropy data (B) as a function of micelle polymer concentration.…”
Section: Resultsmentioning
confidence: 99%
“…Fluorescence anisotropy is a technique that can provide useful information about the rotational motion of a fluorophore through measuring the polarization of the emitted fluorescence. The technique is a proven method for studying molecular mobility and have enabled the use of fluorescence anisotropy in the study of binding interactions, microviscosities of micellar systems, biological membranes, nucleic acids, proteins, and living cells. , Steady-state fluorescence anisotropy measurements are carried out by exciting the sample with vertically plane-polarized light. The parallel and perpendicular components of the fluorescence emission are measured using polarizers oriented with vertical and horizontal orientation, respectively.…”
This work details a study whereby the characterization of chiral selectors and identification of optimal separation conditions is evaluated by steady-state fluorescence anisotropy measurements. Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evaluating chiral recognition, and in this study, the feasibility of characterizing chiral separation systems by the technique is evaluated. Four chiral selectors were examined under various conditions to explore correlation between chiral separation ability and differences in the steady-state fluorescence anisotropy of the enantiomers measured under similar conditions. A good correlation between the fluorescence anisotropy data and separation data was observed with R2 values ranging from 0.9279 to 0.9959. The fluorescence anisotropy measurements were examined under conditions that mimicked chiral separation conditions and the feasibility of a priori optimization of chiral separations is discussed.
“…Identical trends are observed in both cases even though MS detection was used for CE experiments alluding to the fact that the anisotropy experiment could have predicted separation conditions for optimal selectivity as manifested by the chiral resolution in the CE−MS experiment. 10 CE−MS data . (A) anisotropy data (B) as a function of micelle polymer concentration.…”
Section: Resultsmentioning
confidence: 99%
“…Fluorescence anisotropy is a technique that can provide useful information about the rotational motion of a fluorophore through measuring the polarization of the emitted fluorescence. The technique is a proven method for studying molecular mobility and have enabled the use of fluorescence anisotropy in the study of binding interactions, microviscosities of micellar systems, biological membranes, nucleic acids, proteins, and living cells. , Steady-state fluorescence anisotropy measurements are carried out by exciting the sample with vertically plane-polarized light. The parallel and perpendicular components of the fluorescence emission are measured using polarizers oriented with vertical and horizontal orientation, respectively.…”
This work details a study whereby the characterization of chiral selectors and identification of optimal separation conditions is evaluated by steady-state fluorescence anisotropy measurements. Earlier studies in our laboratory have shown fluorescence anisotropy to be an effective tool in evaluating chiral recognition, and in this study, the feasibility of characterizing chiral separation systems by the technique is evaluated. Four chiral selectors were examined under various conditions to explore correlation between chiral separation ability and differences in the steady-state fluorescence anisotropy of the enantiomers measured under similar conditions. A good correlation between the fluorescence anisotropy data and separation data was observed with R2 values ranging from 0.9279 to 0.9959. The fluorescence anisotropy measurements were examined under conditions that mimicked chiral separation conditions and the feasibility of a priori optimization of chiral separations is discussed.
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