Electron‐Transfer Kinetics of Microperoxidase‐11 Covalently Immobilised onto the Surface of Multi‐Walled Carbon Nanotubes by Reactive Landing of Mass‐Selected Ions

Abstract

Controlled deposition of biological molecules on nanostructured materials is a basic step towards the realisation of biochip components. In this study we report the investigation of the first covalent immobilisation of mass-selected redox protein on a carboxyl-functionalised multi-walled carbon nanotube (MWCNT) electrode surface by means of ion soft landing. The immobilised protein maintains its biochemical properties, displaying an excellent electrochemical behaviour on the electrode surface. The deposition of mass-selected ions is influenced by several factors, including the charge state and the collision energy of the projectile ions. To elucidate the mechanism involved in the protein reactive landing onto the MWCNT surface, the data obtained from cyclic voltammetry experiments were modelled according to the Marcus theory. The proposed method opens up the way to the development of a new generation of biocomponents with potential use in biosensors, diagnostics, biofuel cells and bioactive films.

Field-controlled electron transfer and reaction kinetics of the biological catalytic system of microperoxidase-11 and hydrogen peroxide

“…The green CV, compared to the black CV, shows a large increase in the cathodic current, which is indicative of the reduction of H 2 O 2 to water and has been reported previously. 35,36 When V G was turned on, the reduction current further increased as reflected in the blue and red CVs. This effect is consistent with the situation described in Figure 1(c) and the discussion on the enhanced electron transfer due to the reduced effective tunnel barrier height given above.…”

Bioelectrocatalysis by Microperoxidase‐11 in a Multilayer Architecture of Chitosan Embedded Gold Nanoparticles

“…MP-11, obtained by the proteolytic digestion of horse heart cytochrome c, contains a covalently bound heme c as the redox active group that is able to exchange electrons with the electrode [9][10][11][12][13][14][15]. It presents several advantages, such as aqueous solubility, weaker tendency of aggregation, availability of a number of chemical functionalities for covalent coupling and modification [16][17][18][19].…”

Electrochemical Biosensing Based on Carbon Nanotubes

“…The strategy was essentially based on the creation of positively charged moieties onto CNTs by first grafting CNTs with polyether via an in situ cationic ring-opening through an electrostatic interaction and consequently confine such kinds of redox mediators onto CNTs. The prepared nanocomposite with surface-confined Fe(CN) 6 3− redox mediator could be used as efficient electronic transducers for the general development of redox enzyme-based electrochemical biosensors.…”

“…The direct p-p interaction between porphyrins and SWCNTs played an important role in achieving the ordered assembly of protonated porphyrin in the form of J-and H-type aggregates on the SWCNT's surface [12]. A functional composite of SWCNTs with hematin, a water-insoluble porphyrin, was prepared in 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6 ) ionic liquid, which provided an easy way for accelerating the electron transfer, constructing highly sensitive biosensors, and extending the application of water-insoluble porphyrin [13].…”