Electron spin resonance study of changes during the development of a mouse myeloid leukaemia. I. Paramagnetic metal ions

Abstract

Summary.-The blood, spleen and liver of mice were examined by means of electron spin resonance (e.s.r.), throughout the course of myeloid leukaemia induced by intravenous injection of leukaemic spleen cells. In blood, marked increases in the concentrations of iron transferrin and ceruloplasmin occurred within the first 3 days after injection. In the spleen, changes in the concentrations of paramagnetic copper and iron complexes were detectable by about the 5th day, before any measurable splenic enlargement, whilst in the liver changes were detectable by about the 8th day. The changes occurring in blood, spleen and liver during the development of leukaemia appear to be related and they are discussed in terms of iron transport.

ESR study of development of RFM/Un murine myeloid leukaemia

“…Changes in the concentration of paramagnetic species in the blood of RFM/ Un mice developing myeloid leukaemia are similar to those observed in the blood of RF/J mice developing their myeloid leukaemia (Dodd, 1975). The time scale of these changes is consistent with the time scale of the disease.…”

“…About 12 animals were examined for each experimental point. The methods used to prepare samples and record their spectra have been described previously (Dodd, 1975 …”

“…The ESR signals in frozen samples of spleen have been tentatively assigned to various complexes of paramagnetic metal ions (Dodd, 1975). Relative changes in the concentrations of these species during development of the disease are shown in Fig.…”

“…More recently, experiments have examined the changes in the concentration of paramagnetic metal ions (Swartz et al, 1973;Dodd, 1975;Dodd & Silcock, 1976) and a particular free radical, the ascorbyl radical (Dodd, 1973;Dodd & GironConland, 1975;Giron-Conland, 1975;Silcock & Dodd, 1976) during the development of experimental malignancies in mice and rats. This paper reports ESR studies on a murine myeloid leukaemia carried in RFM/Un Inice, and compares the results with those obtained in RF/J mice.…”

Electron spin resonance study of changes during the development of a mouse myeloid leukaemia. II. The ascorbyl radical

“…Consequently a systematic study has been made of the changes in the ascorbyl radical concentration occurring in the blood, spleen and liver during a murine myeloid leukaemia. A parallel study of the changes in the paramagnetic metal ion concentrations, in frozen samples of these tissues, has been reported previously (Dodd, 1975).…”