Electron probe microanalysis of calcium and phosphorus in dense bodies isolated from human platelets

Costa, Jonathan L.; Fay, Denise D.; McGill, Manley

doi:10.1016/0049-3848(81)90101-8

Cited by 7 publications

(5 citation statements)

References 11 publications

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“…The diameter (xfSD) of these granules, measured in a total of 79 structures in three normal subjects, was 181 f 74 nm, with a range of 64-397 nm. This value is in general agreement with previously reported figures ( x f S E ) of 2 2 1 f l l nm (Costa et al, 1981) and…”

Section: Platelet Whole Mountssupporting

confidence: 94%

“…1987). This value is in general agreement with previously reported figures ( x f S E ) of 2 2 1 f l l nm (Costa et al, 1981) and The diameter (xfSD) of these granules, measured in a total of 79 structures in three normal subjects, was 181 f 74 nm, with a range of 64-397 nm.…”

Section: Platelet Whole Mountssupporting

confidence: 92%

“…The typical X-ray spectra obtained from circular dense bodies in normal platelets, ranging from approximately 80 nm to 400 nm in maximum diameter, displayed prominent phosphorus and calcium peaks (Fig 4A), with a mean P/Ca concentration ratio of 1.21f0.05 (Table 111). which is comparable to the previously reported values for platelet whole mounts of 1.43f0.03 (Costa et al, 1979), 1.25f0.02 (Costa et al, 1981) and 1.30f0.04 (Skaer et al, 1976). We found no variation in this ratio with dense granule size (data not shown).…”

Section: Electron Probe Microanalysissupporting

confidence: 92%

“…Weiss (White, 1985: Holmsen. unstained whole mount preparations (Witkop et at, 198 7), probably due to their high calcium content (Skaer et at, 1976: Costa et al 1981. In addition to their intense osmophilia, dense granules are inherently electron opaque when viewed on unfixed.…”

mentioning

confidence: 99%

“…In addition to their intense osmophilia, dense granules are inherently electron opaque when viewed on unfixed. unstained whole mount preparations (Witkop et at, 198 7), probably due to their high calcium content (Skaer et at, 1976: Costa et al 1981. Dense granules are also identified by their high affinity for uranyl ions (uranaffin reaction) (Richards & DaPrada, 1977;Payne, 1984) and by their ability to accumulate the fluorescent dye mepacrine (Lorez et al 1977).…”

mentioning

confidence: 99%

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Heterogeneous abnormalities of platelet dense granule ultrastructure in 20 patients with congenital storage pool deficiency

et al. 1993

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Studies on platelet dense granule structure were carried out in 20 patients with various types of congenital storage pool deficiency (SPD), including 15 with specific deficiencies of dense granules and dense granule substances (delta-SPD), and five with combined deficiencies of dense and alpha-granules (alpha delta-SPD). Dense granules were identified by their high affinity for uranyl ions (uranaffin reaction), by their ability to accumulate the fluorescent dye mepacrine, and by their inherent electron opacity on unfixed, unstained whole mount preparations. By all these methods, dense granules were markedly decreased in seven albino patients with the Hermansky-Pudlak syndrome (HPS) variant of delta-SPD. These findings suggest that the basic defect in these patients is a specific abnormality in organelle development which prevents the formation of an intact granule structure, a quantitative abnormality which may differ from that in animals with related pigment disorders. In contrast, eight non-albino patients with delta-SPD had, on average, only a slightly reduced number of uranaffin-positive and mepacrine-positive granules, but a shift in uranaffin-granule distribution towards those lacking a dense core ('empty granules'), suggesting a more qualitative type of dense granule defect. These results are consistent with previous evidence suggesting a decreased uptake of ATP across the granule membrane in delta-SPD. In addition, on whole mounts, these patients' platelets contained substantial numbers of electron dense chains and clusters which contained P and Ca, but with a P/Ca ratio less than that of typical dense granules, and which were retained, along with a larger amount of ATP, after thrombin treatment of the platelets. The various findings in these patients raise the possibility that these structures may represent microvesicles, derived from the Golgi apparatus, which provide a transport mechanism for concentrating adenine nucleotides and calcium in dense granules and which is impaired in some patients with SPD. Additional defects may account for the more extensive granule abnormalities observed in alpha delta-SPD.

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Section: Platelet Whole Mountssupporting

confidence: 94%

Section: Platelet Whole Mountssupporting

confidence: 92%