Electron-Probe and Electron Microscope Studies of Carious Dentinal Lesions with a Remineralized Surface Layer

Takuma, S.; Ogiwara, Hideaki; Suzuki, H.

doi:10.1159/000260163

Cited by 35 publications

(30 citation statements)

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“…The F-rich outer zone of the root lesion is frequently hypermineralised [Furseth and Johansen, 1968;Takuma et al, 1975;Tohda et al, 1996] but we were not able to detect any increase in Ca concentration in this zone. The reason for this is not clear, but it is also not always apparent with the electron probe in sections that clearly show a hypermineralised zone by microradiography [Tohda et al, 1996].…”

Section: Discussioncontrasting

confidence: 64%

“…Subsequent electron probe studies showed that natural lesions in the root of the tooth have increased F concentrations in the outer surface layer [Takuma et al, 1975;Tohda et al, 1996], but our study provides the first quantitative F data from carious root tissue. In contrast to Levine's [1972] report we found a small but significant increase in F in the body of the lesion over that in the adjacent inner dentine ( fig.…”

Section: Discussionmentioning

confidence: 75%

“…Remineralisation processes that have been reported to occur in root tissues affected by natural caries [Takuma et al, 1975;Schüpbach et al, 1990b] and artificial caries lesions [Wefel et al, 1986;Hoppenbrouwers et al, 1987b] can be a cause for true uptake of F, rather than the consolidation of endogenous F as mentioned above.The outer layer of carious root tissue contains crystals that are larger than normal [Takuma et al, 1975] and may be equivalent to the 'caries crystals' seen in enamel lesions. Such crystals are likely to scavenge F from the lesion fluid during their formation, particularly if there is an external source of F [Dunipace et al, 1994].…”

Section: Discussionmentioning

confidence: 99%

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Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Shu

Pearce²,

Sissons³

et al. 1998

Caries Res

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Proton probe analysis has been used to provide for the first time quantitative F concentration data in carious root tissues from subjects consuming water containing 1 ppm F. In small lesions at the neck of the tooth with minimal tissue loss the F concentration was significantly higher at the outer lesion edge than at the outer edge of adjacent sound root tissue. In one sample with high F values the lesion edge had 19,000 ppm F and the adjacent sound root surface 5,400 ppm F (μg F/g apatite). In large lesions with extensive cavitation F was again concentrated in the outer edge of the lesion and was significantly higher (1,800–4,100 ppm) than in adjacent sound inner dentine (190–290 ppm). Fluoride concentrations varied markedly along the outer edge of both normal and carious root tissues. Fluoride increase at the lesion edge is not an effect of tissue shrinkage but probably a result of remineralisation events during caries. This additional F may be expected to increase tissue resistance to further acid attacks.

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Section: Discussioncontrasting

confidence: 64%

Section: Discussionmentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

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Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Shu

Pearce²,

Sissons³

et al. 1998

Caries Res

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“…Caution is needed in interpretation of the findings of Zn in dentin underneath amalgam fillings because Zn in dentin does not necessarily originate from the amalgam as indicated by Takuma et al 63 In primary carious lesions in dentine they demonstrated an increase of Zn, especially in remineralised surface layers. Except amalgam components external pigments can also cause discoloration of dentin.…”

Section: Discussionmentioning

confidence: 99%

Penetration of amalgam constituents into dentine

Scholtanus

Özcan

Huysmans

2009

Journal of Dentistry

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OBJECTIVES: Amalgam restorations are replaced by adhesively placed composite resin restorations at an increasing rate. After the removal of amalgam dentine often shows marked dark discoloration that is attributed to the penetration of corrosion products from overlying amalgams. It is questioned whether penetration of metals into dentine affects the dentine as a substrate for adhesive procedures. This study has been performed to clarify the origin of dark discoloration of dentine by metals from amalgam with special regards to corrosion products. METHODS: A review of the literature has been performed using Medline database. As keywords dentine and amalgam, subsequently combined with penetration, interface, crevice, interaction, corrosion, were used. This was followed up by extensive hand search using reference lists of relevant articles. RESULTS: Data in the literature have been gathered from extracted amalgam filled teeth and from artificially aged amalgam filled teeth. Corrosion studies have been performed in vivo aged teeth as well as in vitro. Sn is the main element, followed by Zn and Cu, that is consistently found in dentine underneath amalgam, as well as in amalgam corrosion products and in marginal seal deposits. Penetration of elements from amalgam has only been observed in discolored and in demineralised dentine. CONCLUSIONS: Darkly discolored dentine as found underneath amalgam restorations contains amalgam corrosion products and is demineralised. Therefore it must be considered a different substrate for clinical procedures than sound dentine. Penetration of Amalgam Constituents into Dentin ABSTRACTObjective: Amalgam restorations are replaced by adhesively placed composite resin restorations at an increasing rate. After removal of amalgam, dentin often shows marked dark discoloration that is attributed to penetration of corrosion products from overlying amalgams. It is questioned if penetration of metals into dentin effects the dentin as a substrate for adhesive procedures. This study has been performed to clarify the origin of dark discoloration of dentin by metals from amalgam with special regards to corrosion products. Methods:A review of the literature has been performed using Medline database. As keywords dentin and amalgam, subsequently combined with penetration, interface, crevice, interaction, 2 corrosion, were used. This was followed up by extensive hand search using reference lists of relevant articles.Results: Data in the literature have been gathered from extracted amalgam filled teeth and from artificially aged amalgam filled teeth. Corrosion studies have been performed in vivo aged teeth as well as in vitro. Sn is the main element, followed by Zn and Cu, that is consistently found in dentin underneath amalgam, as well as in amalgam corrosion products and in marginal seal deposits. Penetration of elements from amalgam has only been observed in discoloured and in demineralised dentin. Conclusions:Darkly discoloured dentin as found underneath amalgam restorations contains amalgam cor...

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“…The structural changes involved have been stud ied at the light microscope level [Kronfeld, 1939;Gottlieb, 1947;M idler, 1948;Spouge, 1973]. Contact microradiography has shown a radio-dense layer at the surface of arrested lesions [Levine, 1974], Fewer studies, however, have been car ried out with transmission electron micro scopy (TEM) [Holding, 1961;Holding el a!., 1965;Sarnat and Massier, 1965;Takurna et al, 1967;Her ting, 1969;Er min and Neu bauer, 1972;Takuma et al, 1975], crystal lography [Vahl et al, 1964: Newesely, 1965Herting, 1969], electron probe [Tak uma et al, 1975], physico-chemical analysis [Miller and Massier, 1962;Young and Mass ier, 1963;Malone et al, 1966;Levine, 1972Levine, , 1973Bowles and Levine, 1973].…”

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confidence: 99%

Qualitative and Quantitative Data on Arrested Caries in Dentine

et al. 1979

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Identification and indexing of lattice planes through high-resolution transmission electron microscopy provided precise measurements of intertubular crystals of arrested caries in dentine compared to sound dentine. In arrested caries, mean crystal width was 310 Å (SD 44) with a range from 200 to 400 Å. Mean thickness was 86 A (SD 19.3) with a range from 57 to 130 Å. In sound dentine, mean width was not very different at 296 Å (SD 39) with a similar range from 200 to 400Å. Mean thickness was lower: 31.6 Å (SD 5.4) with a range from 24 to 41 Å. A growing process due to thickening of the crystallites was pointed out. The coexistence of whitlockite and apatite within the large crystals of intratubular dentine in arrested caries was shown through high-resolution electron microscopy and micro-electron diffraction. Two stages of arrested caries could be found at the crystal level: firstly, a precipitate of the material dissolved at the very beginning of the carious process, and secondly, remineralization of decalcified dentine by growth of residual crystals (intertubular dentine) and by a maturating process of whitlockite towards apatite (intratubular crystals).

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Electron-Probe and Electron Microscope Studies of Carious Dentinal Lesions with a Remineralized Surface Layer

Cited by 35 publications

References 1 publication

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Fluoride Distribution in Sound and Carious Root Tissues of Human Teeth

Penetration of amalgam constituents into dentine

Qualitative and Quantitative Data on Arrested Caries in Dentine

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