Electron microscopy of two types of gonadotrophs in the anterior pituitary glands of persistent estrous and diestrous rats

Kurosumi, Kazumasa; Oota, Yoshihiko

doi:10.1007/bf00330584

Cited by 84 publications

(31 citation statements)

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Section: Discussionmentioning

confidence: 99%

“…Morphologically the former was characterized by possessing two types of secretory granules, i.e., large and small secretory granules, while the latter by containing only the small type of granule. At first we called the former the FSH cell and the latter, the LH cell (KuROSUMI and OOTA, 1968), but later renamed them Type I and Type II gonadotrophs (KuROSUlvli, 1974), because light microscopic immunocytochemistry indicated that LH and FSH were contained within the same cell (NAKANE, 1970;PHIFER et al, 1973). ToUGARD et al (1973) reported two types of rat gonadotrophs by immunoelectron microscopy using antibodies to LH and its subunits, calling these Type A and Type B, with the former containing two classes of round secretory granules, large and small, which correspond to our FSH cell OOTA, 1968) or Type I (KUROSUMI, 1974), and the latter containing only small secretory granules and corresponding to our LH cells or Type II.…”

Section: Discussionmentioning

confidence: 99%

“…BARNES (1963) demonstrated FSH cells and LH cells in the mouse anterior pituitary, and showed that the secretory granules of FSH cells were less electron dense than those of LH cells. KUROSUMI and OOTA (1968) found two groups of secretory granules, large and small, in rat presumptive FSH cells, but only small secretory granules in the putative LH cells. In mouse gonadotrophs, large secretory granules were not observed by BARNES (1963), though other morphological characteristics of rat and mouse FSH cells were identical.…”

mentioning

confidence: 84%

“…the follicle-stimulating hormone (FSH) and luteinizing hormone (LH). In early morphological studies using either the light or electron microscope, gonadotropinproducing cells also divided into two types, suggesting that one might produce FSH and the other might produce LH (PURVES and GRIESBACH, 1954;FARQUHAR andRINEHART, 1954, 1955;BARNES, 1963;KUROSUMI and OOTA, 1968).BARNES (1963) demonstrated FSH cells and LH cells in the mouse anterior pituitary, and showed that the secretory granules of FSH cells were less electron dense than those of LH cells. KUROSUMI and OOTA (1968) found two groups of secretory granules, large and small, in rat presumptive FSH cells, but only small secretory granules in the putative LH cells.…”

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confidence: 99%

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Immunoelectron Microscopic Studies of Gonadotrophs in the Male and Female Rat Anterior Pituitaries, with Special Reference to their Changes with Aging.

Kurosumi

Ozawa

Akiyama

et al. 1991

Arch. Histol. Cytol.

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Summary.Gonadotrophs immunocytochemically identified with an antibody to LH/3 were studied in both male and female rats of various ages, viz., 2,14 and 23 months after birth. The rat gonadotrophs are classified into two cell types, i.e., Type I containing large (300-700 nm) and small Gonadotropins secreted by the anterior pituitaries of mammals are known to consist of two hormones, viz. the follicle-stimulating hormone (FSH) and luteinizing hormone (LH). In early morphological studies using either the light or electron microscope, gonadotropinproducing cells also divided into two types, suggesting that one might produce FSH and the other might produce LH (PURVES and GRIESBACH, 1954;FARQUHAR andRINEHART, 1954, 1955;BARNES, 1963;KUROSUMI and OOTA, 1968).BARNES (1963) demonstrated FSH cells and LH cells in the mouse anterior pituitary, and showed that the secretory granules of FSH cells were less electron dense than those of LH cells. KUROSUMI and OOTA (1968) found two groups of secretory granules, large and small, in rat presumptive FSH cells, but only small secretory granules in the putative LH cells. In mouse gonadotrophs, large secretory granules were not observed by BARNES (1963), though other morphological characteristics of rat and mouse FSH cells were identical. Immunohistochemistry at the light microscopic level revealed that most gonadotrophic cells in rat (NAKANE, 1970) and man (PHIFER et al., 1973) pituitaries contain both FSH and LH, though a few cells contain only one of these gonadotropins. Because of the colocalization of FSH and LH in some gonadotrophs, KUROSUMI (1974) renamed the previous FSH and LH cells Type I and Type II gonadotrophs, respectively. The authors use this classification here; that is, the Type I gonadotroph contains large and small secretory granules, while the Type II cell contains only small granules.As most typical gonadotrophs in the male rat pituitary that correspond to Type I gonadotrophs contain both LH and FSH in the same cell, the subcellular distribution of the two hormones required clarification. INOUE and KUROSUMI (1984) studied the immunoelectron microscopic localization of LB and 559

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 84%

mentioning

confidence: 99%

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Immunoelectron Microscopic Studies of Gonadotrophs in the Male and Female Rat Anterior Pituitaries, with Special Reference to their Changes with Aging.

Kurosumi

Ozawa

Akiyama

et al. 1991

Arch. Histol. Cytol.

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“…The shape of the 7 Golgi apparatus in basophilic gonadotropes of the anterior pituitary has been described principally as round, which spherically enlarges after castration (Addison, 1917;Ellison and Wolfe, 1934;Severinghaus, 1937Severinghaus, , 1933Wolfe and Brown, 1942). Although the circular profile of the globular Golgi apparatus can be observed in electron micrographs of early studies focusing on pituitary gonadotropes (Farquhar and Rinehart, 1954;Kurosumi and Oota, 1968; see also chapter 4 "Gonadotropes" in Costoff, 1973), little attention has been paid to the significance of its globular shape.…”

Section: Structural Characteristics Of the Golgi Apparatus And Microtmentioning

confidence: 99%

Functional implications of the Golgi and microtubular network in gonadotropes

Watanabe

Bochimoto

Koga

et al. 2014

Molecular and Cellular Endocrinology

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In contrast to the widely accepted images of the Golgi apparatus as a cup-like shape, the Golgi in pituitary gonadotropes is organized as a spherical shape in which the outer and inner faces are cis-and trans-Golgi elements, respectively. At the center of the spherical Golgi, a pair of centrioles is situated as a microtubule-organizing center from which radiating microtubules isotropically extend toward the cell periphery. This review focuses on the significance of the characteristic organization of the Golgi and microtubule network in gonadotropes, considering the roles of microtubule-dependent membrane transport in the formation and maintenance of the Golgi structure. Because the highly symmetrical organization of the Golgi is possibly perturbed in response to experimental treatments of gonadotropes, monitoring of the Golgi structure in gonadotropes under various experimental conditions will be a novel in vivo approach to elucidate the biogenesis of the Golgi apparatus.

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The corticotroph of the rat adenohypophysis: A comparative study

Bowie¹,

Williams²,

Shiino³

et al. 1973

Am. J. Anat.

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The immunological and cytological specificity of antiserum to adrenocorticotrophic hormone was established and the corticotroph defined at the light microscopic level by immunocytochemistry. Our observations substantiated recent reports by several investigators. By comparing thin methacrylate sections with adjacent 1 p-methacrylate sections immunochemically stained with anti-ACTH, the ACTH cell was identified at the ultrastructural level and its morphology studied. Using the criteria established at light and electron microscopic levels, the corticotroph was readily found in tissue which had been fixed in paraformaldehyde-osmium mixture and embedded in epoxy resin. Ultrastructural changes in this cell in response to experimentally designed alterations in t h e pituitary ACTH levels confirmed this identification. The ACT11 cell was likewise identified in glutaraldehyde-fixed material. Thyrotrophs were tentatively defined. Several ultrastructural studies responsible for conflicting reports in the literature were investigated. It was concluded that the corticotroph is a unique cell type and that granule size and morphology remain important criteria for adenohypophyseal cell identification regardless of the fixation used. The need for a definitive immunocytochemical study of thyrotrophs at the ultrastructural level was recognized.

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Electron microscopy of two types of gonadotrophs in the anterior pituitary glands of persistent estrous and diestrous rats

Cited by 84 publications

References 7 publications

Immunoelectron Microscopic Studies of Gonadotrophs in the Male and Female Rat Anterior Pituitaries, with Special Reference to their Changes with Aging.

Immunoelectron Microscopic Studies of Gonadotrophs in the Male and Female Rat Anterior Pituitaries, with Special Reference to their Changes with Aging.

Functional implications of the Golgi and microtubular network in gonadotropes

The corticotroph of the rat adenohypophysis: A comparative study

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