Electron microscopy of the differentiating rat incisor ameloblast

Dynamic behavior of ciliated centrioles in rat incisor ameloblasts during cell differentiation.

“…Previous studies with light microscopes considered that, in undifferentiated ameloblasts, their centriolar pairs were located proximal to the nuclei, and during differentiation, moved past the nuclei into a distal position with Golgi apparatus (RENYI,1933;KALLENBACH, 1971). The present study with the electron microscope, however, revealed that most centrioles in the ameloblasts in the undifferentiated phase, which included many mitotic figures, were located lateral in relation to the nuclei.…”

“…This phase shows many mitotic figures and has been also called the proliferation zone (KALLENBACH, 1971), where ameloblasts are organized into a stratified low-columnar epithelium as in Figure 3. In this phase, 11 ameloblasts were examined with serial sections with regard to their centrioles.…”

“…An ameloblast is one of the cell systems in which movement of the centrioles has been described by light microscopic observation during the course of its differentiation, albeit insufficiently (RENYI, 1933;KALLENBACH, 1971). The present study aims at a more precise observation of the behavior and morphology of the centrioles in the differentiating ameloblasts of the rat.…”

Fine structure of the outer enamel epithelium in the cervical loop of the rat incisor.

“…In the present study, lipid droplets were observed in regions OE-2 and OE-3, being most numerous in OE-2. KALLENBACHE [16] has stated that the basal cell membrane is smooth when observed by electron microscopy. In fact, however, the cell membrane was not smooth.…”

“…Only a few investigations have been made on the outer enamel epithelium using electron microscopy; PANNESE [12], DECKER [13], REITH [14], MOE [15] and KALLENBACH [16] all briefly reported the fine structure of the outer enamel epithelium. These studies showed that the outer enamel epithelium is lined by an outer basal lamina, and that the cells in the outer enamel epithelium have microvilli on their lateral membranes and are connected with adjacent cells by desmosomes.…”