Electron microscopy of biomaterials based on hydroxyapatite

Suvorova, Elena I.; Klechkovskaya, V. V.; Комаров, В. Ф.; Северин, А. В.; Мелихов, И. В.; Buffat, PA

doi:10.1134/s1063774506050191

Cited by 14 publications

(8 citation statements)

References 8 publications

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“…As follows from [36,38,39], at the initial stages of crystallization, the c bases of GA NC slices grow slowly, while the side surfaces accrue rapidly. The water molecules form a hydrate carpet at the flat c faces of the GA slices, which is held by the chemisorp tion forces.…”

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 98%

“…The globular polymers are able to control the GA crystallization, affect the crystal morphology and architecture of the calcium phos phate crystal agglomerates [17,18,22,23,28,29]. Based on the obtained experimental data and by anal ogy with the results of the GA NC formation and agglomeration during the precipitation from the water solutions in the presence of globular proteins and polysaccharides [30][31][32][33][34][35][36][37][38][39], the model of the GA NC nucleation and agglomeration in GA/MC OMC can be presented as follows (Fig. 9).…”

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

“…9, 1) is accompanied with strain and expansion of the MC globules (Fig. 9, 2), interaction of Ca 2+ with the ОНgroups of the MC molecules and formation of primary spheroid calcium phosphate clusters (accord ing to [24,28,33,36,38,39], the [Ca 3 (PO 4 ) 3 ] 3 compo sition at the crystallization centers (Fig. 9, 3).…”

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

“…9, 3). Dehy dration of spheroid clusters respective to the relay crystallization mechanism favors the formation of bi dimensional Ca 8 H 2 (PO 4 ) 6 octacalcium phosphate (OCP) precursors with a thickness of 0.7-2 nm [38,39]. The interaction of OCP with Ca(OH) 2 in the solution leads to the formation of the flat GA NCs with similar sizes and habits (Fig.…”

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

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The influence of methylcellulose (MC) on solubility of calcium hydroxyapatite (HA) crystals in HA/MC nanocomposites

Zakharov

Sentsov

Киселев

et al. 2016

Prot Met Phys Chem Surf

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The influence of methylcellulose ([C 6 H 7 O 2 (OH) 3 -x (OCH 3 ) x ] n , MC) on the morphology and solubility of calcium hydroxyapatite (Ca 10 (PO 4 ) 6 (OH) 2 , GA) nanocrystals (NCs) in GA/MC organomineral nanocomposites (OMCs) is studied. GA/MC OMCs with the MC content of 0.5, 1, 2, 5, 10 and 20 wt % are synthesized in the Ca(OH) 2 -H 3 PO 4 -[C 6 H 7 O 2 (OH) 3 -x (OCH 3 ) x ] n -H 2 O system under biomimetic condi tions (37°C). The composition and structural features of OMCs, as well as crystallographic characteristics, size, and morphology of GA NC in OMCs, are determined via chemical analysis, X ray diffraction (XRD), infrared spectroscopy (IRS), thermal analysis (DTA and DTG), scanning (SEM) and transmission (TEM) electron microscopy, and electron diffraction (ED). It is shown that the growth in the MC concentration in OMCs leads to the change in the GA NC morphology and the increase in their solubility (for Ca 2+ and ions).

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Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 98%

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

Section: The Influence Of MC On the Formation And Growth Of Ga Ncs Inmentioning

confidence: 99%

See 2 more Smart Citations

The influence of methylcellulose (MC) on solubility of calcium hydroxyapatite (HA) crystals in HA/MC nanocomposites

Zakharov

Sentsov

Киселев

et al. 2016

Prot Met Phys Chem Surf

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“…Therefore, their study, analysis and improvements are very important in the medical and biomaterials fields. On the other hand, transmission electron microscopy (TEM) characterization, both in the mode of high resolution electron microscope (HRTEM) and scanning transmission electron microscopy (STEM), is necessary for studying apatite crystals at the nanoscale [6,7]. These techniques include: space group determination, defect characterization and surface characterization [8,9].…”

Section: Introductionmentioning

confidence: 99%

Probe size study of apatite irradiation in stem

Brès

Reyes‐Gasga

Rey

et al. 2014

Eur. Phys. J. Appl. Phys.

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Abstract. The effect of electron beam irradiation of stoichiometric hydroxyapatite (Ca10(PO4)6(OH)2, HAP) is investigated using electron energy loss spectroscopy (EELS) in the scanning transmission electron microscope (STEM) mode equipped with a LaB6 gun. Initial irradiation at 160 A cm −2 dose rate shows no modification of the low loss spectra. No difference of mass loss for Ca, O and P is observed for two different probe diameters: 4 nm (dose rate = 160 A cm −2 ) and 24 nm (dose rate = 70 A cm −2 ). Furthermore no formation of CaO is observed for both experimental conditions. It is proposed that the low values of both electron dose rates and doses (from 350 to 2400 C cm −2 ) avoid mass loss. At the higher dose rate obtained with a field emission gun (FEG), transformations are observed for the lowloss signal as well as for the Ca, P and O signals. These results might be very useful for the future studies of apatite particles at the nanoscale.

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