Electron microscopical studies of frozen-dried yeast

Mundkur, Balaji D.

doi:10.1016/0014-4827(60)90219-6

Cited by 71 publications

(17 citation statements)

References 20 publications

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“…In Saccharomyces cerevisiae, α-D-mannan is a surface component (149) and serves as a surface antigen in many fungi (750). In phosphonomannans some sugar residues are attached through phosphorodiester linkages.…”

Section: Ratio Of Units In Mainmentioning

confidence: 99%

The Chemistry of Polysaccharides of Fungi and Lichens

Gorin¹,

Barreto‐Bergter²

1983

The Polysaccharides

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Section: Ratio Of Units In Mainmentioning

confidence: 99%

The Chemistry of Polysaccharides of Fungi and Lichens

Gorin¹,

Barreto‐Bergter²

1983

The Polysaccharides

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“…However, cytochemical investigations with such material have been published. For example, Nelson has demonstrated adenosine triphosphatase (21) and succinic dehydrogenase (22) in frozendried spermatozoa, and Mundkur has localized polysaccharides (18), nucleic acids (19), and sulphydryl groups (20) in frozen-dried yeast. Both investigators carried out their reactions on the frozen-dried cells before final plastic-embedding and sectioning.…”

Section: Introductionmentioning

confidence: 99%

Ultrathin Frozen Sections

Waldenfels

Leduc

1967

The Journal of Cell Biology

100

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A relatively simple method for obtaining ultrathin, frozen sections for electron microscopy has been developed. Tissues, cultured cells, and bacteria may be employed. They are fixed in 1.25-4% glutaraldehyde for 1-4 hr, are washed overnight in buffer at 3°C, and are embedded in 20% thiolated gelatin or pure gelatin. Before sectioning they are partially dehydrated in 50% glycerol, frozen in liquid nitrogen on a modified tissue holder, and subsequently maintained at -70°C with dry ice. Finally, they are sectioned very rapidly with glass knives on a slightly modified Porter-Blum MT-I microtome in a commercial deep-freeze maintained at -35°C and are floated in the trough of the knife on a 40% solution of dimethylsulfoxide (DMSO). The sections are picked up in plastic loops and transferred to distilled water at room temperature for thawing and removal of the DMSO, placed on grids coated with Formvar and carbon, air-dried, and stained with phosphotungstic acid, sodium silicotungstate, or a triple stain of osmium tetroxide, uranyl acetate, and lead. Large flat sections are obtained in which ultrastructural preservation is good. They are particularly useful for cytochemical studies.

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“…This enzyme is isolated and proves to be capable of breaking down substances in yeast cell walls (BROCK 1964). When seen in the electron microscope the cell walls of yeasts consist of two layers (XORTHCOTE 1954, MUNDKUR 1960. The outer layer is supposed to contain chiefly mannan and chitin, the inner layer containing chiefly glucan.…”

Section: Introductionmentioning

confidence: 99%

Breakdown of the Plant Cell Wall During the Cell-Fusion Process

Sassen¹

1965

Acta Botanica Neerlandica

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The disappearance of cell walls during the cell fusion process in the plant was studied electron microscopically on the following objects.Hordeum vulgare. Differentiation in the central vessel of the root. Achras sapota Origin of laticifers in the shoot apex. Phycomyces blakesleeanus and Hansemda wingei: Zygote formation.In the central vessel of Hordeum the end wall first swells, it is then detached in its entirety from the lateral wall, after which the breakdown process is completed.In Achras, Phycomyces and Hansenula cell wall breakdown during the cell fusion process takes place gradually, beginning in the centre. Enzymes that are in close contact with the plasma membrane probably play a part m this process.Both the skeleton which consists of microfibrils and the matrix of the cell walls are dissolved enzymatically. A cellulolytic enzyme is demonstrated in the root extract of Hordeum, a chitmolytic enzyme m the zygote extract of Phycomyces. CONTENTS

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Electron microscopical studies of frozen-dried yeast

Cited by 71 publications

References 20 publications

The Chemistry of Polysaccharides of Fungi and Lichens

The Chemistry of Polysaccharides of Fungi and Lichens

Ultrathin Frozen Sections

Breakdown of the Plant Cell Wall During the Cell-Fusion Process

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