Electron Microscopic Study of Epidermal Sweat Duct

Visualization of a system of filaments 7-10 nm thick in cultured cells of an epithelioid line (Pt K2) by immunofluorescence microscopy.

“…4a) and show a variety of bizarre branchings and interconnections. They resemble the arrays described by Brecher in Pt K1 cells (8) and also the tonofilaments and prekeratin fibrils present in various epithelia, especially epidermis (11,12,18,21). The filaments in Pt K2 cells differ from microfilaments not only by their greater diameter, but also in other properties.…”

“…The filaments in Pt K2 cells differ from microfilaments not only by their greater diameter, but also in other properties. In cross sections they often show an unstained core which represents an internal hollow channel, as demonstrable by the penetration of phosphotungstic acid into DO.wt) HMouse3T3cells isolated fibers (data not shown), similar to the hollow core de-)-and then stained with scribed in tonofilaments (21). Frequently, single 7-to 10-nm boundary.…”

“…They further exhibit a conspicuous tendency to aggregate laterally and form ;tudies in indirect imheavily stained spicule-or rod-like bundles in which the indi-16), H17 serum gives vidual fibrous constituents are no longer distinguishable ( Fig. n the cytoplasm and 4), a situation which closely resembles the fibril-keratohyalin serum is used on 3T3 granule associations during early steps of keratinization procemid, colchicine, or cesses (11,12,18,21). Like the fibrous bundles of keratinizing )und the nucleus are epithelia, the 7-to 10-nm filaments of Pt K2 cells also show ese bundles increases frequent associations with clusters of ribosomes, probably higher concentrations polyribosomes (Fig.…”

Fine Structure of the Human Sweat Ducts of Eccrine and Apocrine Types

“…Several papers have been published on the electron microscope observations of the human eccrine sweat ducts (HIBBS, 1958;CHARLES, 1960;MUNGER, 1961;ZELICKSON, 1961;HASHIMOTO et al, 1965HASHIMOTO et al, , 1966bHASHIMOTO et al, , 1966cSHIBASAKI and ITO, 1967;ELLIS, 1967ELLIS, , 1968BREATHNACH, 1971). The most reliable and extensive report has, however, been that by HASHIMOTO et al (1966a) on the apocrine sweat duct; the latter has also been dealt with only briefly in studies of whole gland structures (CHARLES, 1959;MUNGER, 1965).…”

“…1). The luminal cell (ZELICKSON, 1961) is also called surface cell (MUNGER, 1961), superficial cell (ELLIS, 1967) or inner cell (HASHIMOTO et al, 1965), but the first terminology is adopted here.…”