Electron Microscopic Studies on Intracellular Multiplication of <i>Rickettsia tsutsugamushi</i> in L Cells

Urakami, Hiroshi; Tsuruhara, Takashi; Tamura, Akira

doi:10.1111/j.1348-0421.1984.tb00777.x

Cited by 37 publications

(27 citation statements)

References 11 publications

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“…The rickettsial morphology (Fig. 8) is the same as those seen in L cell cultures reported previously (6,7). The outer and inner layers of the rickettsial cell wall are recognized as electron-dense and electron-lessdense layers, respectively, corresponding with the proposal by Silverman and Wisseman (3) as the characteristics for R. tsutsugamushi.…”

supporting

confidence: 66%

Electron Microscopic Observations of the Embryo Leptotrombidium (Leptotrombidium) pallidum Naturally Infected with Rickettsia tsutsugamushi

Urakami¹,

Takahashi²,

Tamura³

et al. 1988

Microbiology and Immunology

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Embryos of Leptotrombidium (Leptotrombidium) pallidum mites naturally infected with Rickettsia tsutsugamushi were examined by electron microscopy. Rickettsiae were not found in eggs just after oviposition, but were easily detected in cells at the various parts of the embryos just before hatching, indicating that the rickettsiae are surely vertically transmitted from infected adult mites to the larvae through embryos, and the rickettsiae may multiply in situ during the developing process of the embryo.

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supporting

confidence: 66%

Electron Microscopic Observations of the Embryo Leptotrombidium (Leptotrombidium) pallidum Naturally Infected with Rickettsia tsutsugamushi

Urakami¹,

Takahashi²,

Tamura³

et al. 1988

Microbiology and Immunology

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“…Since most Orientia seen in the lumen were enveloped, it is unlikely that gland cells rupture and release a number of the pathogen at once. Escape by budding was observed in cultured mouse L cells (21,26,29) and mouse peritoneal mesothelial cells (3) infected with O. tsutsugamushi. This study clarified that budding is the process for O. tsutsugamushi escape from the salivary gland cells of vector mites.…”

Section: Discussionmentioning

confidence: 99%

Electron Microscopic Observations of Orientia tsutsugamushi in Salivary Gland Cells of Naturally Infected Leptotrombidium pallidum Larvae during Feeding

Kadosaka

Kimura

2003

Microbiology and Immunology

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We performed a detailed electron microscopic observation on the escaping process of Orientia tsutsugamushi from the salivary gland cells of naturally infected trombiculid larvae into the acinar lumen of the gland during feeding on mice. In unfed larvae, many O. tsutsugamushi were intermingled with secretory granules in the cytoplasm of the salivary gland cell. O. tsutsugamushi was neither found in the acinar lumen nor observed escaping from the apical surface of the gland cell. In contrast, in the larvae fed on mice, many O. tsutsugamushi were observable in the acinar lumen. They were enveloped with the host glandular cell membrane. In salivary gland cells, secretory granules changed the distribution and accumulated in the apical region. In such cells, the majority of O. tsutsugamushi were found at the base of the cell. Some O. tsutsugamushi were pushing the glandular cell membrane outward in various degrees, showing different stages of escape. These findings suggest that larval feeding induced O. tsutsugamushi escape from salivary gland cells, that the escape was by budding, during which O. tsutsugamushi were enveloped in the host cell membrane, and that O. tsutsugamushi would be injected into the mouse skin as a mixture with mite saliva. The study also revealed the presence of many small vesicles that had the same cell wall structure as O. tsutsugamushi in the cytoplasm of the salivary gland cell. Most of them seemed to be products from degenerated Orientia.

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“…Table 1 shows the size of various rickettsiae reported by electron microscopic observations of ultrathin sections. tsutsugamushi (86,89). However, R. tsutsugamushi seems to be a little larger than the others, and the size is in the range of 0.5 to 0.8 gm in width and 1.2 to 3.0 gm in length.…”

Section: ) Morphologymentioning

confidence: 99%

A comparative view of Rickettsia tsutsugamushi and the other groups of rickettsiae

1991

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Recent researches on the rickettsial group microorganisms are summarized in their comparative aspects of morphology, cultivation and multiplication, susceptibility to chemotherapeutics, chemical structure of envelopes, nucleic acid, protein constitution, and gene structures. From this overview, Rickettsia tsutsugamushi seems to have different properties from the others and should be reclassified into a new genus, and a new species name as Orientia tsutsugamushi is proposed.

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Electron Microscopic Studies on Intracellular Multiplication of Rickettsia tsutsugamushi in L Cells

Cited by 37 publications

References 11 publications

Electron Microscopic Observations of the Embryo Leptotrombidium (Leptotrombidium) pallidum Naturally Infected with Rickettsia tsutsugamushi

Electron Microscopic Observations of the Embryo Leptotrombidium (Leptotrombidium) pallidum Naturally Infected with Rickettsia tsutsugamushi

Electron Microscopic Observations of Orientia tsutsugamushi in Salivary Gland Cells of Naturally Infected Leptotrombidium pallidum Larvae during Feeding

A comparative view of Rickettsia tsutsugamushi and the other groups of rickettsiae

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