Electron Microscopic Studies on Bactericidal Effects of Electrolyzed Acidic Water on Bacteria Derived from Kendo Protective Equipment

Osafune, Tetsuaki; Ehara, Tomoko; Ito, Takashi

doi:10.1265/ehpm.11.206

Cited by 3 publications

(1 citation statement)

References 8 publications

(8 reference statements)

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“…The bactericidal effects of EO water on Staphylococcus saprophyticus, Micrococcus luteus and Bacillus sphaericus can be seen by using a scanning electron microscope. The cells treated with electrolyzed acidic water had wrinkled cell wall with round pores in which the cytoplasmic structures were flushed out (Osafune, Ehara, & Ito, 2006). Little reports on the effects of chlorine, pH and ORP values of the EO water in inactivation of pathogens are available.…”

Section: Inactivation Of Microbes Using Eo Watermentioning

confidence: 99%

Application of electrolyzed water in the food industry

Huang¹,

Hung²,

Hsu³

et al. 2008

Food Control

531

360

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Section: Inactivation Of Microbes Using Eo Watermentioning

confidence: 99%

Application of electrolyzed water in the food industry

Huang¹,

Hung²,

Hsu³

et al. 2008

Food Control

531

360

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Analysis of Bacterial Flora in Dohyo Soil

Osafune

Mitsuboshi

Ito

et al. 2007

Environ. Health Prev. Med.

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Objectives: Sumo wrestling is one of the most popular sports in Japan. Injuries are not uncommon as this is a vigorous contact sport. Sumo wrestlers have little in the way of protective clothing; their main garb is the mawashi, making them prone to exposure to any microorganisms in the dohyo. The bacterial flora of the dohyo has received little attention. If the constituent flora is identified, then appropriate treatment or prevention of any bacterial lesions or infections incurred by the wrestlers is possible. Methods: The Vitek AMS system used in this study was developed by McDonnell Douglas Corporation. In this system, the physiological and biochemical properties of Gram-positive and-negative bacilli, Gram-positive and-negative cocci, and fungi isolated from clinical materials and environments are examined using test cards specifically for each microorganism group, and the results are automatically read by a computer and encoded. Obtained codes are compared with a built-in database, and bacterial species of test strains are identified. Results: In this study, using the automatic identification kit VITEK or ATB, we describe the aerobic bacterial flora found in the dohyo over the four seasons of the year. We also investigated the effect of salt on the bacterial flora as sumo wrestlers toss salt on the dohyo before each match. We show the relationship between salinity changes and variations in the flora observed upon the addition of salt. Without salt, at the beginning of a match, Gram-negative bacteria predominate. When salt is added, there is a transient decrease in the incidence of flora followed by an increase in the incidence Grampositive cocci. Conclusions: Sixteen bacterial genera were identified using the bacterial identification systems in dohyo soil samples during the year. The number of identified bacterial species was 32. Even in the presence of salt, there is a measurable amount of bacterial flora in dohyo soil; salt does not act as an antibacterial agent.

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A Scanning Electron Microscopic Method for the Study of Bacterial Growth Inhibition by the Paper Disc Method

et al. 2011

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SummaryWe previously found an inverse correlation between the number of bacteria and the number of fungi collected throughout the year from tatami mats in judo halls. Antibacterial substances produced by the fungi are presumably responsible for the decline in bacterial populations. Culture extracts from fungi isolated from the tatami mats were prepared and their antibacterial activity against Micrococcus luteus, Staphylococcus warneri, and Bacillus subtilis isolated from the mats was determined by the paper disc method. For all 3 bacteria, clear inhibitory zones were obtained indicative of the presence of a growth inhibitory compound in the fungal culture extract. A sampling method was developed for the scanning electron microscopic observation of bacteria around the inhibitory zones produced by the fungal culture extract. Using this method, changes in the surface microstructures of bacteria existing inside the inhibitory zone and growth region were successfully compared by scanning electron microscopy.Key words Bacteria, Fungi, Inhibitory zones in antibacterial assays, Scanning electron microscope.The microbial flora, the number of bacteria and the number of fungi on the tatami mats of the judo hall in Nippon Sport Science University change during the course of a year. The number of bacteria on the tatami mats decreases when the number of fungi increases, and the number of bacteria increases when the number of fungi decreases (Nakamura 2006). This inverse correlation between the number of bacteria and fungi is presumably caused not only by mutual competition for nutrients but also by antibacterial compounds produced during fungal growth. The paper disc method, a simple inexpensive method for determining the antibacterial activity of culture extracts, was used to show that bacteria frequently isolated from the tatami mats of the judo hall are sensitive to compounds in extracts prepared from fungi isolated from the tatami mats. A newly developed sampling method for the scanning electron microscopic analysis of bacteria within the fungal extract inhibitory zone has allowed a comparison of the surface microstructure of bacteria inside the inhibitory zone and those within the growth region. Materials and methods Freeze-drying of fungal cultureThe fungi isolated from the vinyl chloride tatami mats in the judo hall of Nippon Sport Science University were pure-cultured, followed by shake culture in Sabouraud's liquid medium at 27°C for 12 weeks (Nara et al. 2007). Fungi were separated from the culture fluid by centrifugation at 3,000 rpm for 15 min. The culture fluid was filtered through a 0.22-mm membrane (Millipore,

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Electron Microscopic Studies on Bactericidal Effects of Electrolyzed Acidic Water on Bacteria Derived from Kendo Protective Equipment

Cited by 3 publications

References 8 publications

Application of electrolyzed water in the food industry

Application of electrolyzed water in the food industry

Analysis of Bacterial Flora in Dohyo Soil

A Scanning Electron Microscopic Method for the Study of Bacterial Growth Inhibition by the Paper Disc Method

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